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    COMPOSITIONS AND METHODS FOR PROTEIN ISOLATION
    2.
    发明申请
    COMPOSITIONS AND METHODS FOR PROTEIN ISOLATION 审中-公开
    蛋白质分离的组合物和方法

    公开(公告)号：US20080213760A1

    公开(公告)日：2008-09-04

    申请号：US11773353

    申请日：2007-07-03

    申请人： Jeffrey C. Braman ,  Carsten-Peter Carstens ,  Natalia Novoradovskaya ,  Rajesh Bagga ,  Lee Scott Basehore

    发明人： Jeffrey C. Braman ,  Carsten-Peter Carstens ,  Natalia Novoradovskaya ,  Rajesh Bagga ,  Lee Scott Basehore

    IPC分类号： C12Q1/68

    CPC分类号： C07H21/04

    摘要： The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.

    摘要翻译： 本发明提供了包含至少两个标签序列的多核苷酸和载体。 本发明还提供了包含链霉亲和素结合肽序列和钙调蛋白结合肽序列的多核苷酸和载体。 本发明还提供多核苷酸和载体，其中目的基因与至少两个标签序列融合，例如链霉抗生物素蛋白结合肽序列和钙调蛋白结合肽序列。 本发明还提供了由这些多核苷酸编码的嵌合蛋白质。 本发明还提供了使用本发明的多核苷酸检测和/或分离蛋白质复合物或鉴定感兴趣的蛋白质的结合配偶体的方法。

    Compositions and methods for protein isolation
    4.
    发明授权
    Compositions and methods for protein isolation 有权
    蛋白质分离的组成和方法

    公开(公告)号：US07238478B2

    公开(公告)日：2007-07-03

    申请号：US10712137

    申请日：2003-11-13

    申请人： Jeffrey C. Braman ,  Carsten-Peter Carstens ,  Natalia Novoradovskaya ,  Rajesh Bagga ,  Lee Scott Basehore

    发明人： Jeffrey C. Braman ,  Carsten-Peter Carstens ,  Natalia Novoradovskaya ,  Rajesh Bagga ,  Lee Scott Basehore

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12P21/02 ,  C12N15/63 ,  C07K14/00 ,  C07K1/22

    CPC分类号： C07H21/04

    摘要： The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.

    摘要翻译： 本发明提供了包含至少两个标签序列的多核苷酸和载体。 本发明还提供了包含链霉亲和素结合肽序列和钙调蛋白结合肽序列的多核苷酸和载体。 本发明还提供多核苷酸和载体，其中目的基因与至少两个标签序列融合，例如链霉抗生物素蛋白结合肽序列和钙调蛋白结合肽序列。 本发明还提供了由这些多核苷酸编码的嵌合蛋白质。 本发明还提供了使用本发明的多核苷酸检测和/或分离蛋白质复合物或鉴定感兴趣的蛋白质的结合配偶体的方法。

    Compositions and methods for protein isolation
    7.
    发明授权
    Compositions and methods for protein isolation 有权
    蛋白质分离的组成和方法

    公开(公告)号：US07235362B2

    公开(公告)日：2007-06-26

    申请号：US10987388

    申请日：2004-11-12

    申请人： Jeffrey Carl Braman ,  Carsten-Peter Carstens ,  Natalia Novoradovskaya ,  Rajesh Bagga ,  Lee Scott Basehore

    发明人： Jeffrey Carl Braman ,  Carsten-Peter Carstens ,  Natalia Novoradovskaya ,  Rajesh Bagga ,  Lee Scott Basehore

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12P21/02 ,  C12N15/63 ,  C07K1/22

    CPC分类号： C07H21/04

    摘要： The invention provides for polynucleotides and vectors comprising at least two tag sequences. In particular, preferred vectors are viral vectors. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for methods of using the polynucleotides and vectors of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.

    摘要翻译： 本发明提供了包含至少两个标签序列的多核苷酸和载体。 特别地，优选的载体是病毒载体。 本发明还提供了包含链霉亲和素结合肽序列和钙调蛋白结合肽序列的多核苷酸和载体。 本发明还提供多核苷酸和载体，其中目的基因与至少两个标签序列融合，例如链霉抗生物素蛋白结合肽序列和钙调蛋白结合肽序列。 本发明还提供了使用本发明的多核苷酸和载体用于检测和/或分离蛋白质复合物或鉴定感兴趣的蛋白质的结合配偶体的方法。

    Methods for the separation of biological molecules using sulfolane
    8.
    发明授权
    Methods for the separation of biological molecules using sulfolane 有权
    使用环丁砜分离生物分子的方法

    公开(公告)号：US08686129B2

    公开(公告)日：2014-04-01

    申请号：US11688652

    申请日：2007-03-20

    申请人： Jeffrey C. Braman ,  Lee S. Basehore ,  Natalia Novoradovskaya

    发明人： Jeffrey C. Braman ,  Lee S. Basehore ,  Natalia Novoradovskaya

    IPC分类号： C07H21/00

    CPC分类号： C12N15/1003

    摘要： The present invention provides a method for the isolation of biological molecules by the adsorption of the molecules onto a mineral substrate in the presence of an appropriate mixture of salts and sufolane. Preferably, the biological molecules are nucleic acids. Compositions and kits for performing the process according to the invention are also provided.

    摘要翻译： 本发明提供了一种通过在适当的盐和苏木素混合物的存在下将分子吸附到矿物底物上来分离生物分子的方法。 优选地，生物分子是核酸。 还提供了用于执行根据本发明的方法的组合物和试剂盒。

    SYSTEM FOR ISOLATING BIOMOLECULES FROM A SAMPLE
    9.
    发明申请
    SYSTEM FOR ISOLATING BIOMOLECULES FROM A SAMPLE 审中-公开
    从样品中分离生物分子的系统

    公开(公告)号：US20080026451A1

    公开(公告)日：2008-01-31

    申请号：US11764117

    申请日：2007-06-15

    申请人： Jeffrey Braman ,  Lee Basehore ,  Natalia Novoradovskaya

    发明人： Jeffrey Braman ,  Lee Basehore ,  Natalia Novoradovskaya

    IPC分类号： C12N1/08 ,  C12M1/00

    CPC分类号： C12N15/1006

    摘要： The present invention provides an automated system for purification of a substance of interest. The system generally comprises an instrument for moving fluids through the system, a reagent pack for storing fluids, and a purification cartridge. The cartridge comprises two filtration units for binding substances based on different physical properties. The cartridge also comprises rotary valves for control of movement of fluids on the cartridge. In preferred embodiments, the system is useful for purifying RNA from blood samples.

    摘要翻译： 本发明提供了一种用于纯化目的物质的自动化系统。 该系统通常包括用于移动流体通过系统的仪器，用于存储流体的试剂包和净化筒。 该筒包括两个用于基于不同物理性质结合物质的过滤单元。 墨盒还包括用于控制墨盒上的流体运动的旋转阀。 在优选的实施方案中，该系统可用于从血液样品中纯化RNA。

    Normalization of samples for amplification reactions
    10.
    发明申请
    Normalization of samples for amplification reactions 审中-公开
    标准化扩增反应样品

    公开(公告)号：US20060286558A1

    公开(公告)日：2006-12-21

    申请号：US11152775

    申请日：2005-06-15

    申请人： Natalia Novoradovskaya ,  Alexey Novoradovsky ,  Joseph Sorge ,  Lee Basehore ,  Jeffery Braman

    发明人： Natalia Novoradovskaya ,  Alexey Novoradovsky ,  Joseph Sorge ,  Lee Basehore ,  Jeffery Braman

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6851 ,  C12Q1/6888 ,  C12Q2545/101

    摘要： The present invention provides oligonucleotide primers, compositions, methods, and kits for determining the number of cells from which a cell lysate originated. The invention relies on detection and amplification of unique genomic sequences within the genome of an organism of interest to determine the amount of genomic nucleic acid in a sample. The invention can be used to normalize samples from particular cells, cell types, or organism, and provide an accurate basis for comparison of expression of genes across the samples.

    摘要翻译： 本发明提供寡核苷酸引物，组合物，方法和试剂盒，用于确定起源于细胞裂解物的细胞数量。 本发明依赖于感兴趣的生物体的基因组内的独特基因组序列的检测和扩增，以确定样品中基因组核酸的量。 本发明可用于对来自特定细胞，细胞类型或生物体的样品进行标准化，并提供用于比较样品中基因表达的准确基础。