公开(公告)号：US20120003736A1

公开(公告)日：2012-01-05

申请号：US13175667

申请日：2011-07-01

申请人： Jeffrey Stern ,  Sally Temple Stern

发明人： Jeffrey Stern ,  Sally Temple Stern

IPC分类号： C12N5/071 ,  C12N5/095 ,  C12N5/0775 ,  C12N5/0735 ,  C12N5/0797

CPC分类号： C12N5/0696 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0621 ,  C12N5/0623 ,  C12N2500/16 ,  C12N2500/34 ,  C12N2501/115 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1307 ,  C12N2531/00 ,  C12N2533/40

摘要： Methods for culturing undifferentiated mammalian cells, such as stem and progenitor cells, are provided. The methods involve incubating the cell in the presence of a sustained release composition containing at least one growth factor, wherein the sustained release composition continuously releases the growth factor(s), and wherein the presence of the sustained level of growth factor maintains the cell in an undifferentiated state.

摘要翻译： 提供了培养未分化的哺乳动物细胞如茎和祖细胞的方法。 所述方法包括在存在含有至少一种生长因子的持续释放组合物的情况下孵育细胞，其中持续释放组合物连续释放生长因子，并且其中持续水平的生长因子的存在将细胞维持在 未分化状态。

公开(公告)号：US08481308B2

公开(公告)日：2013-07-09

申请号：US13175667

申请日：2011-07-01

申请人： Jeffrey Stern ,  Sally Temple Stern

发明人： Jeffrey Stern ,  Sally Temple Stern

IPC分类号： C12N5/00

CPC分类号： C12N5/0696 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0621 ,  C12N5/0623 ,  C12N2500/16 ,  C12N2500/34 ,  C12N2501/115 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1307 ,  C12N2531/00 ,  C12N2533/40

摘要： Methods for culturing undifferentiated mammalian cells, such as stem and progenitor cells, are provided. The methods involve incubating the cell in the presence of a sustained release composition containing at least one growth factor, wherein the sustained release composition continuously releases the growth factor(s), and wherein the presence of the sustained level of growth factor maintains the cell in an undifferentiated state.

摘要翻译： 提供了培养未分化的哺乳动物细胞如茎和祖细胞的方法。 所述方法包括在存在含有至少一种生长因子的持续释放组合物的情况下孵育细胞，其中持续释放组合物连续释放生长因子，并且其中持续水平的生长因子的存在将细胞维持在 未分化状态。

公开(公告)号：US20120101024A1

公开(公告)日：2012-04-26

申请号：US13282132

申请日：2011-10-26

申请人： Sally Temple Stern ,  Christopher A. Fasano

发明人： Sally Temple Stern ,  Christopher A. Fasano

IPC分类号： A61K38/02 ,  A61P35/00 ,  A61K31/7088 ,  C12N5/079 ,  C12N5/0735

CPC分类号： C12N5/0619 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45

摘要： Methods and compositions for differentiating mammalian stem and progenitor cells are provided. More particularly, methods and compositions for obtaining neural cells from human embryonic stem cells are provided.

摘要翻译： 提供了用于区分哺乳动物干细胞和祖细胞的方法和组合物。 更具体地，提供了用于从人胚胎干细胞获得神经细胞的方法和组合物。