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    SAPAP3 knockout mouse and clinical modeling associated with the SAPAP3 gene
    1.
    发明申请
    SAPAP3 knockout mouse and clinical modeling associated with the SAPAP3 gene 审中-公开
    SAPAP3敲除小鼠和与SAPAP3基因相关的临床模型

    公开(公告)号：US20080113915A1

    公开(公告)日：2008-05-15

    申请号：US11973304

    申请日：2007-10-05

    申请人： Jeffrey Welch ,  Guoping Feng

    发明人： Jeffrey Welch ,  Guoping Feng

    IPC分类号： A01K67/027 ,  A61K38/00 ,  A61P25/00 ,  C12Q1/68 ,  G01N33/00

    CPC分类号： C12Q1/6883 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0356 ,  A61K38/17 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/6896 ,  G01N2800/301

    摘要： Provided is a transgenic knockout mouse whose genome includes a disruption in its endogenous SAPAP3 gene, wherein the disruption results in the mouse exhibiting increased levels of anxiety as compared to a wild type mouse. Also provided are cells derived from the disclosed transgenic knockout mouse. Also provided are methods of identifying a therapeutic agent for the treatment of an individual diagnosed with anxiety, methods for diagnosing an individual with a clinical disorder associated with reduced expression of a SAPAP3 gene product, and methods for treating an individual with a clinical anxiety disorder associated with reduced expression of a SAPAP3 gene product.

    摘要翻译： 提供了一种转基因敲除小鼠，其基因组包含其内源性SAPAP3基因的破坏，其中与野生型小鼠相比，破坏导致小鼠表现出增加的焦虑水平。 还提供了衍生自所公开的转基因敲除小鼠的细胞。 还提供了鉴定用于治疗患有焦虑症的个体的治疗剂的方法，用于诊断与SAPAP3基因产物的表达降低相关的临床病症的个体的方法，以及用于治疗患有临床焦虑症的个体的方法 降低SAPAP3基因产物的表达。

    Nucleic acids encoding drosophila tipE cation channel proteins and recombinant expression of the same
    2.
    发明授权
    Nucleic acids encoding drosophila tipE cation channel proteins and recombinant expression of the same 失效
    核酸编码果蝇tipE阳离子通道蛋白和重组表达相同

    公开(公告)号：US5593862A

    公开(公告)日：1997-01-14

    申请号：US317880

    申请日：1994-10-04

    申请人： Linda M. Hall ,  Guoping Feng

    发明人： Linda M. Hall ,  Guoping Feng

    IPC分类号： C07K14/435 ,  C12N15/12 ,  C12N1/21 ,  C12N5/10

    CPC分类号： C07K14/43581

    摘要： The present invention provides for the isolation of genomic fragments from Drosophila melanogaster encoding tipE protein, which protein is required for expression of functional voltage dependent cation channels. A positional cloning coupled with transformation strategy was used to identify and isolate the tipE gene. A cDNA corresponding to the gene encoding tipE is also provided and characterized. In another aspect of the present invention, there is provided a functional voltage dependent cation channel. Methods for making and using the cation channel are also provided.

    摘要翻译： 本发明提供了从编码tipE蛋白的果蝇黑腹果蝇分离基因组片段，该蛋白质是用于表达功能依赖电压的阳离子通道所必需的。 使用与转化策略相结合的定位克隆来鉴定和分离tipE基因。 还提供了与编码tipE的基因相对应的cDNA并进行表征。 在本发明的另一方面，提供了一种功能依赖电压的通道。 还提供了制造和使用阳离子通道的方法。

    Assays for modulators of drosophila cation channel function
    5.
    发明授权
    Assays for modulators of drosophila cation channel function 失效
    测定果蝇阳离子通道功能的调节剂

    公开(公告)号：US5871940A

    公开(公告)日：1999-02-16

    申请号：US782396

    申请日：1997-01-13

    申请人： Linda M. Hall ,  Guoping Feng

    发明人： Linda M. Hall ,  Guoping Feng

    IPC分类号： C07K14/435 ,  C12N15/12 ,  C12Q1/02

    CPC分类号： C07K14/43581

    摘要： Genomic and cDNA clones corresponding to the tipE gen of Drosophila melanogaster are described. The tipE protein functions in concert with the para gene product, a polypeptide which exhibits similarity to mammalian voltage-dependent sodium channel .alpha. subunits but is not by itself functional. Coexpression of the tipE and para genes in a host cell affords functional cation channels. The invention accordingly provides screening assays for modulators of Drosophila cation channels employing cells expressing both tipE and para polypeptides, useful inter alia to evaluate candidate pesticidal agents.

    摘要翻译： 描述了对应于黑腹果蝇的tipE基因的基因组和cDNA克隆。 tipE蛋白与对基因产物（一种与哺乳动物电压依赖性钠通道α亚基显示相似性，但本身不具有功能的多肽）起作用。 在宿主细胞中表达tipE和para基因提供功能性阳离子通道。 因此，本发明提供了使用表达tipE和para多肽的细胞的果蝇阳离子通道调节剂的筛选测定法，特别用于评价候选杀虫剂。