公开(公告)号：US20070243172A1

公开(公告)日：2007-10-18

申请号：US11734262

申请日：2007-04-11

申请人： Jeong Ra ,  Bong Kim ,  Hang Lee ,  Sang Woo ,  Hanna Park ,  Hyoeun Kim

发明人： Jeong Ra ,  Bong Kim ,  Hang Lee ,  Sang Woo ,  Hanna Park ,  Hyoeun Kim

IPC分类号： A61K35/39 ,  A61K35/34 ,  C12N5/08

CPC分类号： C12N5/0605 ,  C12N5/0607 ,  C12N2501/115 ,  C12N2506/02

摘要： The present invention relates to placenta tissue-derived multipotent stem cells and cell therapeutic agents containing the same. More specifically, to a method for producing placenta stem cells having the following characteristics, the method comprising culturing amnion, chorion, decidua or placenta tissue in a medium containing collagenase and bFGF and collecting the cultured cells: (a) showing a positive immunological response to CD29, CD44, CD73, CD90 and CD105, and showing a negative immunological response to CD31, CD34, CD45 and HLA-DR; (b) showing a positive immunological response to Oct4 and SSEA4; (c) growing attached to plastic, showing a round-shaped or spindle-shaped morphology, and forming spheres in an SFM medium so as to be able to be maintained in an undifferentiated state for a long period of time; and (d) having the ability to differentiate into mesoderm-, endoderm- and ectoderm-derived cells. Also the present invention relates to placenta stem cells obtained using the production method. The inventive multipotent stem cells have the ability to differentiate into muscle cells, vascular endothelial cells, osteogenic cells, nerve cells, satellite cells, fat cells, cartilage-forming cells, osteogenic cells, or insuline-secreting pancreatic β-cells, and thus are effective for the treatment of muscular diseases, osteoporosis, osteoarthritis, nervous diseases, diabetes and the like, and are useful for the formation of breast tissue.

摘要翻译： 本发明涉及胎盘组织衍生的多能干细胞和含有其的细胞治疗剂。 更具体地，涉及具有以下特征的胎盘干细胞的制造方法，其特征在于，在含有胶原酶和bFGF的培养基中培养羊膜，绒毛膜，蜕膜或胎盘组织，并收集培养的细胞：（a）显示出阳性免疫应答 CD29，CD44，CD73，CD90和CD105，并显示对CD31，CD34，CD45和HLA-DR的消极免疫反应; （b）对Oct4和SSEA4显示出阳性免疫应答; （c）生长附着在塑料上，显示出圆形或纺锤形的形态，并在SFM培养基中形成球体，以便能够长时间保持在未分化状态; 和（d）具有分化成中胚层，内胚层和外胚层衍生细胞的能力。 本发明还涉及使用该制备方法获得的胎盘干细胞。 本发明的多能干细胞具有分化成肌肉细胞，血管内皮细胞，成骨细胞，神经细胞，卫星细胞，脂肪细胞，软骨形成细胞，成骨细胞或胰岛素分泌胰腺β-细胞的能力，因此是 有效治疗肌肉疾病，骨质疏松症，骨关节炎，神经疾病，糖尿病等，并且可用于形成乳房组织。

公开(公告)号：US08871513B2

公开(公告)日：2014-10-28

申请号：US13810334

申请日：2011-07-18

申请人： Jeong Chan Ra ,  Sung Keun Kang ,  Ju Yeon Seo ,  Hyoeun Kim

发明人： Jeong Chan Ra ,  Sung Keun Kang ,  Ju Yeon Seo ,  Hyoeun Kim

IPC分类号： C12N5/071 ,  C12N5/073 ,  C12N5/0775

CPC分类号： C12N5/0605 ,  C12N5/0668 ,  C12N2500/14 ,  C12N2500/32 ,  C12N2501/115 ,  C12N2501/33 ,  C12N2501/39

摘要： The present invention relates to a medium for culturing mesenchymal stem cells, and more particularly to a medium composition for culturing mesenchymal stem cells, which contains basal medium, L-ascorbic acid 2-phosphate, fetal bovine serum, basic fibroblast growth factor (b-FGF), non-essential amino acids (NEAAs), insulin, N-acetyl-L-cysteine, calcium chloride, and hydrocortisone, and a method of culturing mesenchymal stem cells using the same. According to the present invention, a number of mesenchymal stem cells required for stem cell therapy can be obtained in a short time, and the ability of mesenchymal stem cells to differentiate is improved so that they are useful for stem cell therapy.

摘要翻译： 本发明涉及培养间充质干细胞的培养基，更具体地涉及培养间充质干细胞的培养基组合物，其含有基础培养基，L-抗坏血酸2-磷酸，胎牛血清，碱性成纤维细胞生长因子（b- FGF），非必需氨基酸（NEAAs），胰岛素，N-乙酰基-L-半胱氨酸，氯化钙和氢化可的松，以及使用其的培养间充质干细胞的方法。 根据本发明，可以在短时间内获得干细胞治疗所需的多种间充质干细胞，提高间充质干细胞分化的能力，使其可用于干细胞治疗。

公开(公告)号：US20130164849A1

公开(公告)日：2013-06-27

申请号：US13810334

申请日：2011-07-18

申请人： Jeong Chan Ra ,  Sung Keun Kang ,  Ju Yeon Seo ,  Hyoeun Kim

发明人： Jeong Chan Ra ,  Sung Keun Kang ,  Ju Yeon Seo ,  Hyoeun Kim

IPC分类号： C12N5/071

CPC分类号： C12N5/0605 ,  C12N5/0668 ,  C12N2500/14 ,  C12N2500/32 ,  C12N2501/115 ,  C12N2501/33 ,  C12N2501/39

摘要： The present invention relates to a medium for culturing mesenchymal stem cells, and more particularly to a medium composition for culturing mesenchymal stem cells, which contains basal medium, L-ascorbic acid 2-phosphate, fetal bovine serum, basic fibroblast growth factor (b-FGF), non-essential amino acids (NEAAs), insulin, N-acetyl-L-cysteine, calcium chloride, and hydrocortisone, and a method of culturing mesenchymal stem cells using the same. According to the present invention, a number of mesenchymal stem cells required for stem cell therapy can be obtained in a short time, and the ability of mesenchymal stem cells to differentiate is improved so that they are useful for stem cell therapy.

摘要翻译： 本发明涉及培养间充质干细胞的培养基，更具体地涉及培养间充质干细胞的培养基组合物，其含有基础培养基，L-抗坏血酸2-磷酸，胎牛血清，碱性成纤维细胞生长因子（b- FGF），非必需氨基酸（NEAAs），胰岛素，N-乙酰基-L-半胱氨酸，氯化钙和氢化可的松，以及使用其的培养间充质干细胞的方法。 根据本发明，可以在短时间内获得干细胞治疗所需的多种间充质干细胞，提高间充质干细胞分化的能力，使其可用于干细胞治疗。