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1.发明申请Nucleotide Compositions Comprising Photocleavable Markers And Methods Of Preparation Thereof 审中-公开包含可分光标记的核苷酸组合物及其制备方法公开(公告)号：US20090263868A1
公开(公告)日：2009-10-22
申请号：US12485213
申请日：2009-06-16
CPC分类号： A61K49/0032 , A61K49/0021 , A61K49/0052 , C07H19/00 , C07H19/04 , C07H21/02 , C07H21/04
摘要： Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable marker nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.
摘要翻译： 描述了用于核酸测序的标记核苷酸和多核苷酸。 描述了制备可光切割标记核苷酸和可光切割标记 - 多核苷酸缀合物的方法。 可将这种可光切割的标记核苷酸掺入核酸中，以产生可光切割的标记 - 多核苷酸缀合物。
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2.发明授权Methods for the preparation of chemically misaminoacylated tRNA via protective groups 有权通过保护基制备化学失氨基酰化tRNA的方法公开(公告)号：US07524941B2
公开(公告)日：2009-04-28
申请号：US11900323
申请日：2007-09-11
IPC分类号： C07C245/00 , C07C291/00 , C12Q1/68 , C07K14/00 , G01N33/00 , C07H21/04
CPC分类号： C07H21/02 , C07H21/04 , G01N33/532 , Y02P20/55
摘要： The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.
摘要翻译： 本发明涉及用于制备化学氨基酰化tRNA以便将标记物引入新生蛋白质的方法。 本发明还涉及在使用化学氨酰化tRNA的细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物。 标记可以包含可切割部分，可检测标记，报告基因特性，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区别。
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3.发明授权Nucleotide compositions comprising photocleavable markers and methods of preparation thereof 有权包含可光切割标记的核苷酸组合物及其制备方法公开(公告)号：US07547530B2
公开(公告)日：2009-06-16
申请号：US11351996
申请日：2006-02-09
CPC分类号： A61K49/0032 , A61K49/0021 , A61K49/0052 , C07H19/00 , C07H19/04 , C07H21/02 , C07H21/04
摘要： Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.
摘要翻译： 描述了用于核酸测序的标记核苷酸和多核苷酸。 描述了制备可光切割标记核苷酸和可光切割标记 - 多核苷酸缀合物的方法。 可将这种可光切割的标记核苷酸掺入核酸中，以产生可光切割的标记 - 多核苷酸缀合物。
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4.发明授权公开(公告)号：US07057031B2
公开(公告)日：2006-06-06
申请号：US10193781
申请日：2002-07-12
CPC分类号： A61K49/0032 , A61K49/0021 , A61K49/0052 , C07H19/00 , C07H19/04 , C07H21/02 , C07H21/04
摘要： Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.
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5.发明申请Methods for the preparation of chemically misaminoacylated tRNA via protective groups 有权通过保护基制备化学失氨基酰化tRNA的方法公开(公告)号：US20080287710A1
公开(公告)日：2008-11-20
申请号：US11900323
申请日：2007-09-11
IPC分类号： C07C235/44
CPC分类号： C07H21/02 , C07H21/04 , G01N33/532 , Y02P20/55
摘要： The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.
摘要翻译： 本发明涉及用于制备化学氨基酰化tRNA以便将标记物引入新生蛋白质的方法。 本发明还涉及在使用化学氨酰化tRNA的细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物。 标记可以包含可切割部分，可检测标记，报告基因特性，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区别。
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6.发明授权Methods for the preparation of chemically misaminoacylated tRNA via protective groups 有权通过保护基制备化学失氨基酰化tRNA的方法公开(公告)号：US07288372B2
公开(公告)日：2007-10-30
申请号：US10345664
申请日：2003-01-16
CPC分类号： C07H21/02 , C07H21/04 , G01N33/532 , Y02P20/55
摘要： The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.
摘要翻译： 本发明涉及用于制备化学氨基酰化tRNA以便将标记物引入新生蛋白质的方法。 本发明还涉及在使用化学氨酰化tRNA的细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物。 标记可以包含可切割部分，可检测标记，报告基因特性，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区别。
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公开(公告)号：US08148062B2
公开(公告)日：2012-04-03
申请号：US11639121
申请日：2006-12-14
IPC分类号： C12Q1/68
CPC分类号： A61K41/0042 , A61K38/00 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6883 , G01N33/58
摘要： The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may used for the detection of diseases and disorders.
摘要翻译： 本发明涉及用于在细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物，或由蛋白质合成机理识别的物质。 标记可以包含可切割部分，可检测标记，报告基因特性，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区别。 本发明还包括使用可以用于治疗人类和其他哺乳动物疾病和病症的药物组合物中的使用错氨基酰化tRNA制备的蛋白质，以及可用于检测疾病和病症的试剂盒。
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公开(公告)号：US07485427B1
公开(公告)日：2009-02-03
申请号：US11879077
申请日：2007-07-16
CPC分类号： C12Q1/6876 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/58 , C12Q2523/319
摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.
摘要翻译： 本发明涉及可用于从复杂混合物检测和分离靶组分的试剂和缀合物，例如来自生物样品的核酸，来自体液的细胞和来自翻译反应的新生蛋白。 试剂包含与光反应性部分结合的可检测部分。 缀合物包括通过共价界限偶联到底物的试剂，其可以通过施用电磁辐射被选择性地切割。 目标可以用可检测分子标记的物质可以很容易地识别，并与物质的异源混合物分离。 缀合物暴露于放射线以功能形式释放靶，完全不变。 使用可光致发光的分子前体作为共轭物，标记可以并入大分子中，分离出新生大分子，并将标记完全除去。 本发明还涉及用这些缀合物分离的靶，其可用作可以施用于人和其它哺乳动物的药剂或组合物。 有用的组合物包括生物制剂如核酸，蛋白质，脂质和细胞因子。 缀合物也可用于监测药物组合物在体内和诊断，治疗和预防目的中的途径和半衰期。 本发明还涉及可用于检测疾病，病症和物质复杂背景中的几乎任何单一物质的试剂和缀合物的试剂盒。
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公开(公告)号：US07423122B2
公开(公告)日：2008-09-09
申请号：US10993568
申请日：2004-11-19
IPC分类号： C07K17/00
CPC分类号： G01N33/582 , C07K16/40 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Methods are described for incorporating N-terminal, C-terminal and (optionally) affinity markers into a nascent protein
摘要翻译： 本发明涉及促进在细胞或无细胞翻译系统内翻译的新生蛋白的检测和分析的非放射性标记物。 可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。 描述了将N-末端，C-末端和（任选地）亲和标记掺入到新生蛋白中的方法
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公开(公告)号：US07312038B2
公开(公告)日：2007-12-25
申请号：US11326021
申请日：2006-01-05
CPC分类号： C12Q1/6876 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/58 , C12Q2523/319
摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.
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