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    Hydroxyapatite-Binding Peptides for Bone Growth and Inhibition
    3.
    发明申请
    Hydroxyapatite-Binding Peptides for Bone Growth and Inhibition 有权
    羟基磷灰石结合肽用于骨生长和抑制

    公开(公告)号：US20080279908A1

    公开(公告)日：2008-11-13

    申请号：US11720427

    申请日：2005-11-29

    申请人： Carolyn R. Bertozzi ,  Jie Song ,  Seung-Wuk Lee

    发明人： Carolyn R. Bertozzi ,  Jie Song ,  Seung-Wuk Lee

    IPC分类号： A61K47/48 ,  C07K7/06 ,  C07K7/08 ,  A61F2/00 ,  A61P19/00

    CPC分类号： C07K7/08 ,  A61K9/0024 ,  A61K38/00 ,  A61L27/227 ,  C07K7/06

    摘要： Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp)x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals ˜40 nm in size.

    摘要翻译： 使用组合噬菌体文库显示选择羟基磷灰石（HA）结合肽。 获得在每两个或三个氨基酸序列中具有周期性羟基侧链的伪重复共有氨基酸序列。 这些序列类似于人类I型胶原蛋白（天然骨细胞外基质的主要成分）的（Gly-Pro-Hyp）重复序列。 还观察到碱性氨基酸残基的一致存在。 肽通过固相合成法合成，然后用于模板驱动的HA矿化。 显微镜显示，肽模板大小的多晶HA晶体的生长〜40nm。

    Hydroxyapatite-binding peptides for bone growth and inhibition
    4.
    发明授权
    Hydroxyapatite-binding peptides for bone growth and inhibition 有权
    用于骨生长和抑制的羟基磷灰石结合肽

    公开(公告)号：US08022040B2

    公开(公告)日：2011-09-20

    申请号：US11720427

    申请日：2005-11-29

    申请人： Carolyn R. Bertozzi ,  Jie Song ,  Seung-Wuk Lee

    发明人： Carolyn R. Bertozzi ,  Jie Song ,  Seung-Wuk Lee

    IPC分类号： A61K38/08

    CPC分类号： C07K7/08 ,  A61K9/0024 ,  A61K38/00 ,  A61L27/227 ,  C07K7/06

    摘要： Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp)x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals ˜40 nm in size.

    摘要翻译： 使用组合噬菌体文库显示选择羟基磷灰石（HA）结合肽。 获得在每两个或三个氨基酸序列中具有周期性羟基侧链的伪重复共有氨基酸序列。 这些序列类似于人类I型胶原（天然骨细胞外基质的主要成分）的（Gly-Pro-Hyp）×重复。 还观察到碱性氨基酸残基的一致存在。 肽通过固相合成法合成，然后用于模板驱动的HA矿化。 显微镜显示，肽模板大小约40nm的多晶HA晶体的生长。

    POLYNUCLEOTIDES ENCODING ANTI-SULFOTYROSINE ANTIBODIES
    5.
    发明申请
    POLYNUCLEOTIDES ENCODING ANTI-SULFOTYROSINE ANTIBODIES 有权
    编码抗硫霉素抗体的多核苷酸

    公开(公告)号：US20100009441A1

    公开(公告)日：2010-01-14

    申请号：US12560294

    申请日：2009-09-15

    申请人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    发明人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    IPC分类号： C12N5/06 ,  C12N15/11 ,  C12N15/00

    CPC分类号： C07K16/44 ,  C07K2317/56 ,  C07K2317/622

    摘要： The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

    摘要翻译： 本发明提供能够检测和分离酪氨酸硫酸化的多肽的抗硫代酪氨酸特异性抗体。 本发明的硫代酪氨酸抗体和抗体片段可用于使用任何数量的免疫学测定法，例如ELISA，免疫印迹，Western印迹，免疫沉淀等来区分这些蛋白质的非硫酸化和硫酸化形式。 使用噬菌体展示系统，产生单链抗体（scFv）并筛选抗酪氨酸硫酸化的合成肽抗原，导致分离特异性识别含磺酰酪氨酸的肽和/或在酪氨酸硫酸化蛋白中显示磺酰酪氨酸特异性结合的scFv 。 使用来自一种这样的磺基酪氨酸特异性scFv的VH和VL基因产生全长磺酰酪氨酸特异性免疫球蛋白。

    Anti-sulfotyrosine antibodies
    6.
    发明授权
    Anti-sulfotyrosine antibodies 有权
    抗硫代酪氨酸抗体

    公开(公告)号：US07589182B1

    公开(公告)日：2009-09-15

    申请号：US11900689

    申请日：2007-09-12

    申请人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    发明人： Carolyn R. Bertozzi ,  John Kehoe ,  Andrew M. Bradbury

    IPC分类号： C07K16/00 ,  C07K16/46 ,  A61K39/395

    CPC分类号： C07K16/44 ,  C07K2317/56 ,  C07K2317/622

    摘要： The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

    摘要翻译： 本发明提供能够检测和分离酪氨酸硫酸化的多肽的抗硫代酪氨酸特异性抗体。 本发明的硫代酪氨酸抗体和抗体片段可用于使用任何数量的免疫学测定法，例如ELISA，免疫印迹，Western印迹，免疫沉淀等来区分这些蛋白质的非硫酸化和硫酸化形式。 使用噬菌体展示系统，产生单链抗体（scFv）并筛选抗酪氨酸硫酸化的合成肽抗原，导致分离特异性识别含磺酰酪氨酸的肽和/或在酪氨酸硫酸化蛋白中显示磺酰酪氨酸特异性结合的scFv 。 使用来自一种这样的磺基酪氨酸特异性scFv的VH和VL基因产生全长磺酰酪氨酸特异性免疫球蛋白。

    Compositions and methods for introducing effectors to pathogens and cells
    7.
    发明授权
    Compositions and methods for introducing effectors to pathogens and cells 失效
    用于将病原体引入病原体和细胞的组合物和方法

    公开(公告)号：US5212075A

    公开(公告)日：1993-05-18

    申请号：US686342

    申请日：1991-04-15

    申请人： Mark D. Bednarski ,  Carolyn R. Bertozzi ,  Jon O. Nagy

    发明人： Mark D. Bednarski ,  Carolyn R. Bertozzi ,  Jon O. Nagy

    IPC分类号： A61K47/48 ,  C07D495/04

    CPC分类号： C07D495/04 ,  A61K47/4823 ,  Y10S514/888

    摘要： Compositions and methods for the inhibition and prevention of pathogenic infection and neoplastic disease are provided. The compositions include hybrid molecules having a binding moiety and an effector moiety joined by a linker region. When administered to a host, the binding moiety, such as a carbohydrate, attaches to a receptor, such as a conserved lectin receptor on the pathogen or neoplastic cell, and the effector moiety provides an invariant antigenic determinant for eliciting or modulating an immune response. The effector moiety may also be a drug or other compound which inhibits growth of a bound pathogen or cell. Compositions comprising the hybrid molecule in a suitable pharmaceutical carrier are also provided.

    摘要翻译： 提供了抑制和预防病原体感染和肿瘤疾病的组合物和方法。 组合物包括具有结合部分和通过连接区连接的效应部分的杂合分子。 当施用于宿主时，结合部分（例如碳水化合物）附着于受体，例如病原体或肿瘤细胞上的保守的凝集素受体，并且效应部分提供用于引发或调节免疫应答的不变抗原决定簇。 效应部分还可以是抑制结合的病原体或细胞生长的药物或其它化合物。 还提供了包含合适的药物载体中的杂合分子的组合物。

    Interfacing nanostructures to biological cells
    8.
    发明授权
    Interfacing nanostructures to biological cells 有权
    将纳米结构与生物细胞接触

    公开(公告)号：US08257932B2

    公开(公告)日：2012-09-04

    申请号：US12034388

    申请日：2008-02-20

    申请人： Xing Chen ,  Carolyn R. Bertozzi ,  Alexander K. Zettl

    发明人： Xing Chen ,  Carolyn R. Bertozzi ,  Alexander K. Zettl

    IPC分类号： G01N33/53

    CPC分类号： C12N5/0006 ,  A61K47/549 ,  A61K47/58 ,  A61K47/61 ,  A61K47/6415 ,  A61K47/6949 ,  B82Y5/00 ,  C12N2501/59

    摘要： Disclosed herein are methods and materials by which nanostructures such as carbon nanotubes, nanorods, etc. are bound to lectins and/or polysaccharides and prepared for administration to cells. Also disclosed are complexes comprising glycosylated nanostructures, which bind selectively to cells expressing glycosylated surface molecules recognized by the lectin. Exemplified is a complex comprising a carbon nanotube functionalized with a lipid-like alkane, linked to a polymer bearing repeated α-N-acetylgalactosamine sugar groups. This complex is shown to selectively adhere to the surface of living cells, without toxicity. In the exemplified embodiment, adherence is mediated by a multivalent lectin, which binds both to the cells and the α-N-acetylgalactosamine groups on the nanostructure.

    摘要翻译： 本文公开了纳米结构如碳纳米管，纳米棒等与凝集素和/或多糖结合并制备用于细胞的方法和材料。 还公开了包含糖基化纳米结构的复合物，其选择性结合表达由凝集素识别的糖基化表面分子的细胞。 示例性的是包含与脂质类烷烃官能化的碳纳米管的复合物，其与具有重复的α-N-乙酰半乳糖胺糖基团的聚合物连接。 该复合物显示出选择性地附着于活细胞表面，无毒性。 在示例性实施方案中，粘附是由多价凝集素介导的，其与纳米结构上的细胞和α-N-乙酰半乳糖胺基团结合。

    Interfacing Nanostructures to Biological Cells
    10.
    发明申请
    Interfacing Nanostructures to Biological Cells 有权
    将纳米结构与生物细胞接触

    公开(公告)号：US20080199399A1

    公开(公告)日：2008-08-21

    申请号：US12034388

    申请日：2008-02-20

    申请人： Xing Chen ,  Carolyn R. Bertozzi ,  Alexander K. Zettl

    发明人： Xing Chen ,  Carolyn R. Bertozzi ,  Alexander K. Zettl

    IPC分类号： A61K49/00 ,  C12N5/06 ,  A61P43/00

    CPC分类号： C12N5/0006 ,  A61K47/549 ,  A61K47/58 ,  A61K47/61 ,  A61K47/6415 ,  A61K47/6949 ,  B82Y5/00 ,  C12N2501/59

    摘要： Disclosed herein are methods and materials by which nanostructures such as carbon nanotubes, nanorods, etc. are bound to lectins and/or polysaccharides and prepared for administration to cells. Also disclosed are complexes comprising glycosylated nanostructures, which bind selectively to cells expressing glycosylated surface molecules recognized by the lectin. Exemplified is a complex comprising a carbon nanotube functionalized with a lipid-like alkane, linked to a polymer bearing repeated α-N-acetylgalactosamine sugar groups. This complex is shown to selectively adhere to the surface of living cells, without toxicity. In the exemplified embodiment, adherence is mediated by a multivalent lectin, which binds both to the cells and the α-N-acetylgalactosamine groups on the nanostructure.

    摘要翻译： 本文公开了纳米结构如碳纳米管，纳米棒等与凝集素和/或多糖结合并制备用于细胞的方法和材料。 还公开了包含糖基化纳米结构的复合物，其选择性结合表达由凝集素识别的糖基化表面分子的细胞。 示例性的是包含与脂质类烷烃官能化的碳纳米管的复合物，其与具有重复的α-N-乙酰半乳糖胺糖基团的聚合物连接。 该复合物显示出选择性地附着于活细胞表面，无毒性。 在示例性实施方案中，粘附是由多价凝集素介导的，其与纳米结构上的细胞和α-N-乙酰半乳糖胺基团结合。