摘要:
It is an object of the present invention to provide novel binding molecules for factor VIII and factor VIII-like proteins. Preferred binding molecules of the present invention exhibit not only distinct characteristics for binding of the target factor VIII polypeptides but also specific and desirable characteristics for release (elution) of the target polypeptides. Especially preferred binding molecules according to the invention are short polypeptide sequences, characterized by a stable loop structure.
摘要:
Methods are provided for purification of Factor VIII polypeptides by affinity chromatography and ion exchange chromatography, in which the eluate from the affinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The affinity matrix may comprise a monoclonal antibody or a peptide ligand. The methods result in improved purification without significant yield loss.
摘要:
This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.
摘要:
This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.
摘要:
An automatic banking machine operates responsive to data read from data bearing records to cause financial transfers. The machine includes a card reader that reads data corresponding to financial accounts from user cards, and carries out transactions related to the accounts. The machine includes a camera, a microphone, and the card reader at a customer interface located at the front of the machine. A servicer display device is at a servicer interface located at the rear of the machine. The customer interface and servicer interface can be placed in audio/visual communication. Although a front servicer may not be directly visible to a rear servicer, they are still able to communicate with each other through the customer interface and servicer interface during machine servicing. The servicer interface can also be used to allow a lone servicer to be audibly/visually alerted to a potential customer approaching the machine.
摘要:
An automatic banking machine includes a card reader for reading user cards, and a cash dispenser. A movable camera is positionable within the ATM housing. The automated banking machine carries out transactions related to user accounts based on information read from cards. Service personnel are enabled to direct the camera toward regions of machine components while conducting service activities that are not directly visible by a servicer. A camera assembly includes a microphone to sense sounds associated with machine components during test operations. The generated data may be used by an on-site servicer, or sent to an entity at a remote location. The visual images may be used to enhance diagnostic and servicing capabilities.
摘要:
An automatic banking machine operates responsive to data read from data bearing records to cause financial transfers. The machine includes a card reader that reads data corresponding to financial accounts from user cards and carries out transactions related to the accounts. The machine includes a camera, a microphone, and the card reader at a customer interface located at the front of the machine. A servicer display device is at a servicer interface located at the rear of the machine. The customer interface and the servicer interface can be placed in audio/visual communication. Although a first servicer located at the customer interface may not be directly visible to a second servicer located at the servicer interface, they are still able to communicate through the customer interface and servicer interface during machine servicing.
摘要:
A cash dispensing automated banking machine, such as an ATM, has leveling legs. The legs are also used to fasten the ATM to a shipping pallet. Each leg has a support foot and an opposite free end. The free end is inserted through an opening in an intermediate shipping plate. With the shipping plate situated between the foot and the ATM underside, the free end is screwed into an underside opening. The leg is rotated until the plate is raised to be secure against the underside. The plate is then set on the pallet and fastened thereto by lag screws. The pallet includes horizontally adjustable stabilizing feet at sides thereof. The pallet feet can be extended outward to decrease the ability of a supported ATM to tip over. When not needed, such as during pallet storage, the feet can be retracted inward to reduce pallet size.
摘要:
An automatic banking machine includes a card reader for reading user cards, and a cash dispenser. A movable camera is positionable within the ATM housing. The automated banking machine carries out transactions related to user accounts based on information read from cards. Service personnel are enabled to direct the camera toward regions of machine components while conducting service activities that are not directly visible by a servicer. A camera assembly includes a microphone to sense sounds associated with machine components during test operations. The generated data may be used by an on-site servicer, or sent to an entity at a remote location. The visual images may be used to enhance diagnostic and servicing capabilities.
摘要:
Electrical interfaces, addressing schemes, and command protocols allow for communications with memory modules in computing devices such as imaging and printing devices. Memory modules may be assigned an address through a set of discrete voltages. One, multiple, or all of the memory modules may be addressed with a single command, which may be an increment counter command, a write command, a punch out bit field, or a cryptographic command. The commands may be transmitted using a broadcast scheme or a split transaction scheme. The status of the memory modules may be determined by sampling a single signal that may be at a low, high, or intermediate voltage level.