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1.发明申请Method for Enhancing Extracellular Secretion of Recombinant Proteins in Escherichia coli by Co-expressing Thermobifida Fusca Cutinase 有权通过共表达Thermobifida Fusca角质酶在大肠杆菌中增强重组蛋白的细胞外分泌的方法公开(公告)号：US20140011239A1
公开(公告)日：2014-01-09
申请号：US13935519
申请日：2013-07-04
申请人： Jing Wu , Lingqia Su , Jian Chen
发明人： Jing Wu , Lingqia Su , Jian Chen
CPC分类号： C12N9/20 , C12N9/2408 , C12N9/2414 , C12N9/2434 , C12N9/248 , C12P21/00 , C12P21/02
摘要： The present invention provides a method of increasing extracellular secretion of secretory proteins by co-expressing the secretory proteins with a mature cutinase. Cutinase can improve the permeability of E. coli cell membrane without destroying the membrane, and thus facilitate cross-membrane transfer of the secretory proteins co-expressed in E. coli. Increased extracellular secretion of target proteins can shorten cell culture time, reduce the formation of inclusion bodies and increase production of target proteins.
摘要翻译： 本发明提供了通过用成熟角质酶共表达分泌蛋白来增加分泌蛋白的细胞外分泌的方法。 角质酶可以改善大肠杆菌细胞膜的通透性，而不破坏膜，从而促进大肠杆菌中共表达的分泌蛋白的跨膜转移。 靶蛋白的细胞外分泌增加可以缩短细胞培养时间，减少包涵体的形成并增加靶蛋白的产生。
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公开(公告)号：US11773383B2
公开(公告)日：2023-10-03
申请号：US17349933
申请日：2021-06-17
申请人： Jing Wu , Lingqia Su , Yan Huang
发明人： Jing Wu , Lingqia Su , Yan Huang
CPC分类号： C12N9/18 , C12N9/1051 , C12N9/92 , C12N15/75 , C12Y204/01245 , C12Y301/01074 , C12Y503/01005
摘要： Disclosed is a method for promoting extracellular expression of proteins in B. subtilis using cutinase, which belongs to the technical fields of genetic engineering, enzyme engineering and microbial engineering. It teaches co-expressing a cutinase mutant and a target protein in B. subtilis to promote extracellular expression of the target protein which is naturally located inside cells. The target protein includes xylose isomerase, 4,6-α-glucosyltransferase, 4-α-glucosyltransferase, trehalose synthase, branching enzyme and the like. The invention can achieve extracellular expression of intracellularly localized target protein, improve the production efficiency, reduce the production cost and simplify the subsequent extraction process.
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公开(公告)号：US09963824B2
公开(公告)日：2018-05-08
申请号：US15051677
申请日：2016-02-24
申请人： Jing Wu , Lingqia Su , Ruoyu Hong
发明人： Jing Wu , Lingqia Su , Ruoyu Hong
CPC分类号： D21C5/027 , D21C5/005 , Y02W30/648
摘要： The present invention provides methods for deinking wastepaper by combined use of cutinase and chemical reagents, which relates to the field of enzyme engineering. The method comprises the following steps: pulp preparation, enzymatic hydrolysis, pulp washing and dewatering, and flotation. The enzyme for enzymatic hydrolysis is cutinase at a concentration of 10-20 U/g absolute dry pulp; and 0.5-4% Na2SiO3, 0.1-0.8% MgSO4, 0.1-0.8% EDTA, 0.1-4% H2O2 are used in the chemical treatment. With combined use of enzymatic and chemical treatment, the present invention has solved the problem of the current enzymatic method for deinking that requires large dosage of enzymes and thus high cost. Through proper choice of the kind and the amount of chemical reagents, synergistic effects of the enzymatic and chemical treatment can be achieved, thus increasing the effectiveness of the deinking process. In addition, this method does not require alkaline chemicals, which alleviates the problem of sewage treatment associated with conventional deinking methods.
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公开(公告)号：US20170137995A1
公开(公告)日：2017-05-18
申请号：US15051677
申请日：2016-02-24
申请人： Jing Wu , Lingqia Su , Ruoyu Hong
发明人： Jing Wu , Lingqia Su , Ruoyu Hong
CPC分类号： D21C5/027 , D21C5/005 , Y02W30/648
摘要： The present invention provides methods for deinking wastepaper by combined use of cutinase and chemical reagents, which relates to the field of enzyme engineering. The method comprises the following steps: pulp preparation, enzymatic hydrolysis, pulp washing and dewatering, and flotation. The enzyme for enzymatic hydrolysis is cutinase at a concentration of 10-20 U/g absolute dry pulp; and 0.5-4% Na2SiO3, 0.1-0.8% MgSO4, 0.1-0.8% EDTA, 0.1-4% H2O2 are used in the chemical treatment. With combined use of enzymatic and chemical treatment, the present invention has solved the problem of the current enzymatic method for deinking that requires large dosage of enzymes and thus high cost. Through proper choice of the kind and the amount of chemical reagents, synergistic effects of the enzymatic and chemical treatment can be achieved, thus increasing the effectiveness of the deinking process. In addition, this method does not require alkaline chemicals, which alleviates the problem of sewage treatment associated with conventional deinking methods.
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5.发明申请公开(公告)号：US20220135957A1
公开(公告)日：2022-05-05
申请号：US17349933
申请日：2021-06-17
申请人： Jing Wu , Lingqia Su , Yan Huang
发明人： Jing Wu , Lingqia Su , Yan Huang
摘要： Disclosed is a method for promoting extracellular expression of proteins in B. subtilis using cutinase, which belongs to the technical fields of genetic engineering, enzyme engineering and microbial engineering. It teaches co-expressing a cutinase mutant and a target protein in B. subtilis to promote extracellular expression of the target protein which is naturally located inside cells. The target protein includes xylose isomerase, 4,6-α-glucosyltransferase, 4-α-glucosyltransferase, trehalose synthase, branching enzyme and the like. The invention can achieve extracellular expression of intracellularly localized target protein, improve the production efficiency, reduce the production cost and simplify the subsequent extraction process.
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