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134 条记录 (耗时 0.026 秒)

    Methods for Promoting Extracellular Expression of Proteins in Bacillus subtilis Using a Cutinase
    2.
    发明申请
    Methods for Promoting Extracellular Expression of Proteins in Bacillus subtilis Using a Cutinase 有权

    公开(公告)号：US20220135957A1

    公开(公告)日：2022-05-05

    申请号：US17349933

    申请日：2021-06-17

    申请人： Jing Wu ,  Lingqia Su ,  Yan Huang

    发明人： Jing Wu ,  Lingqia Su ,  Yan Huang

    IPC分类号： C12N9/18 ,  C12N9/92 ,  C12N9/10 ,  C12N15/75

    摘要： Disclosed is a method for promoting extracellular expression of proteins in B. subtilis using cutinase, which belongs to the technical fields of genetic engineering, enzyme engineering and microbial engineering. It teaches co-expressing a cutinase mutant and a target protein in B. subtilis to promote extracellular expression of the target protein which is naturally located inside cells. The target protein includes xylose isomerase, 4,6-α-glucosyltransferase, 4-α-glucosyltransferase, trehalose synthase, branching enzyme and the like. The invention can achieve extracellular expression of intracellularly localized target protein, improve the production efficiency, reduce the production cost and simplify the subsequent extraction process.

    Methods for deinking wastepaper by combined use of cutinase and chemical reagents
    3.
    发明申请
    Methods for deinking wastepaper by combined use of cutinase and chemical reagents 有权

    公开(公告)号：US20170137995A1

    公开(公告)日：2017-05-18

    申请号：US15051677

    申请日：2016-02-24

    申请人： Jing Wu ,  Lingqia Su ,  Ruoyu Hong

    发明人： Jing Wu ,  Lingqia Su ,  Ruoyu Hong

    IPC分类号： D21C5/02 ,  D21C9/18 ,  D21C5/00 ,  D21C9/02

    CPC分类号： D21C5/027 ,  D21C5/005 ,  Y02W30/648

    摘要： The present invention provides methods for deinking wastepaper by combined use of cutinase and chemical reagents, which relates to the field of enzyme engineering. The method comprises the following steps: pulp preparation, enzymatic hydrolysis, pulp washing and dewatering, and flotation. The enzyme for enzymatic hydrolysis is cutinase at a concentration of 10-20 U/g absolute dry pulp; and 0.5-4% Na2SiO3, 0.1-0.8% MgSO4, 0.1-0.8% EDTA, 0.1-4% H2O2 are used in the chemical treatment. With combined use of enzymatic and chemical treatment, the present invention has solved the problem of the current enzymatic method for deinking that requires large dosage of enzymes and thus high cost. Through proper choice of the kind and the amount of chemical reagents, synergistic effects of the enzymatic and chemical treatment can be achieved, thus increasing the effectiveness of the deinking process. In addition, this method does not require alkaline chemicals, which alleviates the problem of sewage treatment associated with conventional deinking methods.

    Methods for deinking wastepaper by combined use of cutinase and chemical reagents
    4.
    发明授权
    Methods for deinking wastepaper by combined use of cutinase and chemical reagents 有权

    公开(公告)号：US09963824B2

    公开(公告)日：2018-05-08

    申请号：US15051677

    申请日：2016-02-24

    申请人： Jing Wu ,  Lingqia Su ,  Ruoyu Hong

    发明人： Jing Wu ,  Lingqia Su ,  Ruoyu Hong

    IPC分类号： D21C9/02 ,  D21C5/02 ,  D21C5/00

    CPC分类号： D21C5/027 ,  D21C5/005 ,  Y02W30/648

    摘要： The present invention provides methods for deinking wastepaper by combined use of cutinase and chemical reagents, which relates to the field of enzyme engineering. The method comprises the following steps: pulp preparation, enzymatic hydrolysis, pulp washing and dewatering, and flotation. The enzyme for enzymatic hydrolysis is cutinase at a concentration of 10-20 U/g absolute dry pulp; and 0.5-4% Na2SiO3, 0.1-0.8% MgSO4, 0.1-0.8% EDTA, 0.1-4% H2O2 are used in the chemical treatment. With combined use of enzymatic and chemical treatment, the present invention has solved the problem of the current enzymatic method for deinking that requires large dosage of enzymes and thus high cost. Through proper choice of the kind and the amount of chemical reagents, synergistic effects of the enzymatic and chemical treatment can be achieved, thus increasing the effectiveness of the deinking process. In addition, this method does not require alkaline chemicals, which alleviates the problem of sewage treatment associated with conventional deinking methods.

    Method for Enhancing Extracellular Secretion of Recombinant Proteins in Escherichia coli by Co-expressing Thermobifida Fusca Cutinase
    5.
    发明申请
    Method for Enhancing Extracellular Secretion of Recombinant Proteins in Escherichia coli by Co-expressing Thermobifida Fusca Cutinase 有权
    通过共表达Thermobifida Fusca角质酶在大肠杆菌中增强重组蛋白的细胞外分泌的方法

    公开(公告)号：US20140011239A1

    公开(公告)日：2014-01-09

    申请号：US13935519

    申请日：2013-07-04

    申请人： Jing Wu ,  Lingqia Su ,  Jian Chen

    发明人： Jing Wu ,  Lingqia Su ,  Jian Chen

    IPC分类号： C12N9/20 ,  C12P21/00

    CPC分类号： C12N9/20 ,  C12N9/2408 ,  C12N9/2414 ,  C12N9/2434 ,  C12N9/248 ,  C12P21/00 ,  C12P21/02

    摘要： The present invention provides a method of increasing extracellular secretion of secretory proteins by co-expressing the secretory proteins with a mature cutinase. Cutinase can improve the permeability of E. coli cell membrane without destroying the membrane, and thus facilitate cross-membrane transfer of the secretory proteins co-expressed in E. coli. Increased extracellular secretion of target proteins can shorten cell culture time, reduce the formation of inclusion bodies and increase production of target proteins.

    摘要翻译： 本发明提供了通过用成熟角质酶共表达分泌蛋白来增加分泌蛋白的细胞外分泌的方法。 角质酶可以改善大肠杆菌细胞膜的通透性，而不破坏膜，从而促进大肠杆菌中共表达的分泌蛋白的跨膜转移。 靶蛋白的细胞外分泌增加可以缩短细胞培养时间，减少包涵体的形成并增加靶蛋白的产生。

    Method for preparing resistant dextrin by using a starch branching enzyme and a cyclodextrin glycosyltransferase
    6.
    发明授权
    Method for preparing resistant dextrin by using a starch branching enzyme and a cyclodextrin glycosyltransferase 有权

    公开(公告)号：US10988550B2

    公开(公告)日：2021-04-27

    申请号：US16196768

    申请日：2018-11-20

    申请人： Jing Wu ,  Sheng Chen ,  Jun Liu

    发明人： Jing Wu ,  Sheng Chen ,  Jun Liu

    IPC分类号： C08B30/18 ,  A23L29/269 ,  C12P19/04 ,  C08L3/02 ,  C08B31/00

    摘要： Disclosed is a method for preparing a resistant dextrin product by using compound enzyme treatment. Starch is first subjected to high-temperature acidolysis to obtain pyrodextrin which is used as the substrate for the enzyme treatment. A compound enzyme reaction system including a starch branching enzyme and a CGTase is used for preparing the resistant dextrin product. The starch branching enzyme and the CGTase are added simultaneously or sequentially to treat the pyrodextrin to further increase the content of the resistant component in the product. The content of the resistant component of the enzyme treated product reaches up to 65.3%, a 21.3% increase from that of the pyrodextrin before the enzyme treatment.

    ANTENNA SYSTEM WITH FULL METAL BACK COVER
    8.
    发明申请
    ANTENNA SYSTEM WITH FULL METAL BACK COVER 审中-公开

    公开(公告)号：US20170117614A1

    公开(公告)日：2017-04-27

    申请号：US15283530

    申请日：2016-10-03

    申请人： Jing Wu ,  Jianchun Mai ,  Chao Wang

    发明人： Jing Wu ,  Jianchun Mai ,  Chao Wang

    IPC分类号： H01Q1/24 ,  H01Q1/38 ,  H01Q1/48 ,  H01Q1/22 ,  H01Q1/36

    CPC分类号： H01Q1/243 ,  H01Q1/2291 ,  H01Q5/328 ,  H01Q9/42 ,  H01Q21/28 ,  H01Q21/30

    摘要： An antenna system with a full metal back cover is provided in the present disclosure. The antenna system includes a metal back cover with a main body, a first sidewall and a second sidewall opposite to each other, a circuit board with a main ground point, a first antenna module and a second antenna module electrically connected to the circuit board. A first gap is formed between the first sidewall and the main body, and a second gap is formed between the second sidewall and the main body; the metal back cover is divided into a first metal portion including the first sidewall and a second metal portion including the second sidewall. The first metal portion includes a top slit penetrating through an edge of the first sidewall, and the second metal portion includes a bottom slit penetrating through an edge of the second sidewall.

    Method, system and device for secured access to protected digital material
    9.
    发明授权
    Method, system and device for secured access to protected digital material 有权
    用于安全访问受保护数字材料的方法，系统和设备

    公开(公告)号：US09064102B2

    公开(公告)日：2015-06-23

    申请号：US11853555

    申请日：2007-09-11

    申请人： Jing Wu ,  James Ronald Flesch

    发明人： Jing Wu ,  James Ronald Flesch

    IPC分类号： G06F21/34 ,  H04L9/32 ,  H04W12/04 ,  H04L29/06

    CPC分类号： G06F21/34 ,  H04L9/3226 ,  H04L65/4084 ,  H04L2209/60 ,  H04L2209/80 ,  H04W12/04

    摘要： A method, system and device for providing secure access to multimedia content received by a networked digital storage device, such as a set-top box. A mobile device, such as a mobile telephone, obtains appropriate security binding information and application software when coupled to the networked digital storage device at its end user location. The mobile device uploads the security binding information to a randomly located temporary hosting device at its place-shifted location when coupled thereto and, through a logical binding with the temporary hosting device, enables a secure, remote session. The secure binding and transfer of appropriate keys allow the remote hosting device to securely access premium or protected digital material/services available at the networked digital storage device. After completion of the secure, remote access session, termination occurs and the security binding information and the computing activity residue can be removed from the mobile device and the temporary hosting device.

    摘要翻译： 一种用于提供对联网的数字存储设备（诸如机顶盒）所接收的多媒体内容的安全访问的方法，系统和设备。 诸如移动电话的移动设备在其最终用户位置处耦合到联网数字存储设备时获得适当的安全绑定信息和应用软件。 移动设备在耦合到其位置移动位置时将安全绑定信息上传到随机定位的临时托管设备，并且通过与临时托管设备的逻辑绑定来启用安全的远程会话。 适当密钥的安全绑定和传输允许远程托管设备安全地访问网络数字存储设备上可用的高级或受保护的数字材料/服务。 在完成安全的远程访问会话之后，终止并且可以从移动设备和临时托管设备移除安全绑定信息和计算活动残留。