公开(公告)号：US08565475B2

公开(公告)日：2013-10-22

申请号：US13243422

申请日：2011-09-23

Applicant: John A. Moon ,  Martin A. Putnam

Inventor： John A. Moon ,  Martin A. Putnam

IPC: G06K9/00 ,  G06K5/00

CPC classification number: G06K7/10821 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00704 ,  B82Y20/00 ,  G01N15/1468 ,  G02B1/005 ,  G06E3/005 ,  G06K7/10722

Abstract: An optical system for reading encoded microbeads. The microbeads provide output light signals onto a Fourier plane when illuminated by an incident light. The system includes an input light source that is configured to illuminate the microbeads thereby providing the output light signals. The output light signals are configured to be projected onto the Fourier plane in a readable manner. The system also includes a reading device that is positioned to detect the output light signals from the Fourier plane. The system also includes a processor that is configured to perform Fourier plane analysis of the optical light signals to determine corresponding codes of the microbeads.

Abstract translation: 用于读取编码微珠的光学系统。 当由入射光照射时，微珠将输出光信号提供到傅立叶平面上。 该系统包括被配置为照亮微珠从而提供输出光信号的输入光源。 输出光信号被配置为以可读方式投影到傅立叶平面上。 该系统还包括读取装置，其被定位成检测来自傅里叶平面的输出光信号。 该系统还包括处理器，其被配置为对光信号进行傅立叶平面分析以确定微珠的相应代码。

公开(公告)号：US08333325B2

公开(公告)日：2012-12-18

申请号：US13014298

申请日：2011-01-26

Applicant: John A. Moon ,  David R. Fournier ,  Martin A. Putnam ,  Alan D Kersey ,  Tuo Li

Inventor： John A. Moon ,  David R. Fournier ,  Martin A. Putnam ,  Alan D Kersey ,  Tuo Li

IPC: G06K7/10

CPC classification number: G06K7/12 ,  G06K19/06084 ,  G06K19/06178 ,  G06K2019/06225

Abstract: An optical reader system including a source light assembly that has a code-reading beam and a fluorescence-excitation beam that are configured to illuminate encoded substrates. The substrates have optically readable codes that provide output signals when the code-reading beam is incident thereon. The output signals are indicative of the codes. The reader system also includes a fluorescence detector that is configured to detect fluorescent signals from the substrates and code pickup optics that are configured to project the output signals from the optically readable codes onto a Fourier plane. The reader system also includes a code detector that is positioned to detect the output signals in the Fourier plane.

Abstract translation: 一种光学读取器系统，包括具有代码读取光束的源光组件和被配置为照亮编码的基板的荧光激发光束。 衬底具有光学可读代码，当代码读取光束入射时，其提供输出信号。 输出信号表示代码。 读取器系统还包括荧光检测器，其被配置为检测来自基板的荧光信号和被配置为将来自光学可读代码的输出信号投影到傅立叶平面上的代码拾取光学器件。 读取器系统还包括代码检测器，其被定位成检测傅立叶平面中的输出信号。

公开(公告)号：US07900836B2

公开(公告)日：2011-03-08

申请号：US12174490

申请日：2008-07-16

Applicant: John A. Moon ,  David R. Fournier ,  Martin A. Putnam ,  Alan D. Kersey ,  Tuo Li

Inventor： John A. Moon ,  David R. Fournier ,  Martin A. Putnam ,  Alan D. Kersey ,  Tuo Li

IPC: G06K7/10

CPC classification number: G06K7/12 ,  G06K19/06084 ,  G06K19/06178 ,  G06K2019/06225

Abstract: An optical reader system that includes a plurality of substrates. The substrates have an optically readable code disposed therein and a source light assembly that is configured to illuminate the substrates with a code-reading beam and another beam for detecting another optically readable property of the substrate. The code-reading beam and the other beam form beam spots on the substrates that have different shapes. The system also includes a reader that is configured to receive output signals from the code-reading beam and the other beam when the substrates are illuminated. The output signals from the code-reading beam are indicative of the code.

Abstract translation: 一种包括多个基板的光学读取器系统。 基板具有设置在其中的光学可读代码，以及源光组件，其被配置为用代码读取光束照射基板，另一个光束用于检测基板的另一光学可读性。 代码读取光束和另一个光束在具有不同形状的基板上形成光束点。 该系统还包括读取器，其被配置为当基板被照亮时从代码读取光束和另一个光束接收输出信号。 代码读取波束的输出信号表示代码。

公开(公告)号：US07843567B2

公开(公告)日：2010-11-30

申请号：US12644255

申请日：2009-12-22

Applicant: John A. Moon ,  Alan D. Kersey ,  Martin A. Putnam ,  Tuo Li

Inventor： John A. Moon ,  Alan D. Kersey ,  Martin A. Putnam ,  Tuo Li

IPC: G01N21/25

CPC classification number: G01N33/552 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/7743 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/54366 ,  G01N33/54386 ,  G03H1/0005 ,  G03H1/02 ,  G03H1/0236 ,  G03H1/26 ,  G03H2001/0033 ,  G03H2001/0044 ,  G03H2210/53 ,  G03H2210/63 ,  G03H2230/10 ,  G03H2250/12 ,  G06K19/04 ,  G06K19/06009

Abstract: A method of identifying an analyte. The method includes providing a plurality of microparticles. The microparticles have optically detectable codes extending along bodies of the corresponding microparticle. The microparticles have the chemical probes attached thereto. Each of the chemical probes is associated with a corresponding one of the codes. The method also includes selectively binding target analytes to the chemical probes on the microparticles to produce labeled microparticles and distributing the labeled microparticles to random locations of a substrate. The method also includes determining the codes for the labeled microparticles in the random array and code positions of the codes in the random array. The method further includes detecting the label on the labeled microparticles in the random array and label positions of the labels in the random array. The method also includes using the code positions and the label positions to analyze the target analyte.

Abstract translation: 识别分析物的方法。 该方法包括提供多个微粒。 微粒具有沿相应微粒的主体延伸的光学检测码。 微粒具有附着于其上的化学探针。 每个化学探针与相应的一个代码相关联。 该方法还包括将目标分析物选择性地结合到微粒上的化学探针以产生标记的微粒并将标记的微粒分布到基底的随机位置。 该方法还包括确定随机阵列中的标记微粒的代码和随机阵列中代码的代码位置。 该方法还包括随机阵列中标记的微粒上的标记和随机阵列中的标记的标记位置的检测。 该方法还包括使用代码位置和标签位置来分析目标分析物。

公开(公告)号：US07791802B2

公开(公告)日：2010-09-07

申请号：US12144209

申请日：2008-06-23

Applicant: Martin A. Putnam ,  John A. Moon

Inventor： Martin A. Putnam ,  John A. Moon

IPC: G02B5/18

CPC classification number: G02B6/021 ,  G06K19/06018 ,  G06K19/06046

Abstract: An optical identification element including a non-waveguide optical substrate. The optical substrate has a volume and includes an inner region surrounded by an outer region. The inner region has an index of refraction that prevents the optical substrate from forming an optical waveguide. The optical substrate includes a diffraction grating within the volume, and the grating provides an output signal indicative of a code when illuminated by an incident light.

Abstract translation: 一种包括非波导光学基片的光学识别元件。 光学基板具有体积并且包括被外部区域包围的内部区域。 内部区域具有防止光学基板形成光波导的折射率。 光学基板包括体积内的衍射光栅，并且当由入射光照射时，光栅提供指示代码的输出信号。

公开(公告)号：US20090034078A1

公开(公告)日：2009-02-05

申请号：US12144209

申请日：2008-06-23

Applicant: Martin A. Putnam ,  John A. Moon

Inventor： Martin A. Putnam ,  John A. Moon

IPC: G02B5/18 ,  G06K19/06

CPC classification number: G02B6/021 ,  G06K19/06018 ,  G06K19/06046

Abstract: An optical identification element including a non-waveguide optical substrate. The optical substrate has a volume and includes an inner region surrounded by an outer region. The inner region has an index of refraction that prevents the optical substrate from forming an optical waveguide. The optical substrate includes a diffraction grating within the volume, and the grating provides an output signal indicative of a code when illuminated by an incident light.

Abstract translation: 一种包括非波导光学基片的光学识别元件。 光学基板具有体积并且包括被外部区域包围的内部区域。 内部区域具有防止光学基板形成光波导的折射率。 光学基板包括体积内的衍射光栅，并且当由入射光照射时，光栅提供指示代码的输出信号。

公开(公告)号：US07901630B2

公开(公告)日：2011-03-08

申请号：US11226914

申请日：2005-09-13

Applicant: Martin A. Putnam ,  John A. Moon ,  Tuo Li

Inventor： Martin A. Putnam ,  John A. Moon ,  Tuo Li

IPC: G01N33/00 ,  G01N33/556 ,  G03H1/00 ,  C12M1/34 ,  C12M3/00

CPC classification number: G01N21/4788 ,  G01N21/6428 ,  G03H1/26 ,  G03H2001/0033

Abstract: An assay stick 7 includes a transparent reaction vessel or tube 14 having one or more microbeads 8 disposed therein. The microbeads 8 have a plurality of unique identification digital codes based on a diffraction grating 12 disposed therein that are detected when illuminated by incident light 24. The incident light 24 may be directed transversely onto the side or onto an end of the tube 14 with a narrow band (single wavelength) or multiple wavelength source, in which case the code is represented by a spatial distribution of light or a wavelength spectrum, respectively. The assay stick 7 may be reused or disposed upon completion of the assay. Alternatively, the beads may be attached to a strip or planar surface. The encoded beads can also provide traceability, quality-control, and authenticity of each bead 8 to its source and/or to the chemistry on each bead 8. Also, the low sample volume of the assay stick allows for faster reactions and better sensitivity.

Abstract translation: 测定棒7包括其中设置有一个或多个微珠8的透明反应容器或管14。 微珠8具有基于设置在其中的衍射光栅12的多个独特的识别数字代码，其被入射光24照射时检测。入射光24可以横向地被引导到管14的一侧或一端上，其中 窄带（单波长）或多波长源，在这种情况下，码分别由光或波长光谱的空间分布表示。 测定棒7可以在测定完成后重复使用或放置。 或者，珠可以附接到条或平面表面。 编码的珠粒还可以提供每个珠粒8到其来源和/或每个珠粒8上的化学物质的可追溯性，质量控制和真实性。而且，测定棒的低样本体积允许更快的反应和更好的灵敏度。

公开(公告)号：US20110003394A1

公开(公告)日：2011-01-06

申请号：US12876927

申请日：2010-09-07

Applicant: ALAN D. KERSEY ,  John A. Moon ,  Martin A. Putnam

Inventor： ALAN D. KERSEY ,  John A. Moon ,  Martin A. Putnam

IPC: G01N21/75 ,  B32B5/16 ,  G01N33/00

CPC classification number: G03F7/001 ,  G02B5/1857 ,  G02B5/203 ,  G03H1/18 ,  G03H1/182 ,  G03H2230/10 ,  Y10S359/90 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T436/143333

Abstract: An encoded microparticle including an optical substrate comprising a material that permits light to propagate therethrough. The optical substrate has an elongated body that extends in a direction along a central axis. The optical substrate includes an outer region that extends about the central axis. The encoded microparticle also includes an optically detectable code that is disposed within the optical substrate and extends along the central axis. The outer region surrounds the optically detectable code about the central axis. The optically detectable code is readable when the light propagates through the outer region and is at least one of reflected or filtered by the optically detectable code. Said at least one of reflected or filtered light propagates through the outer region to be detected for reading the optically detectable code.

Abstract translation: 包含光学基片的编码微粒包括允许光从其中传播的材料。 光学基板具有沿着中心轴线的方向延伸的细长主体。 光学基板包括围绕中心轴线延伸的外部区域。 编码的微粒还包括光学可检测的代码，其设置在光学基底内并沿着中心轴线延伸。 外部区域围绕围绕中心轴的光学检测码。 当光传播通过外部区域并且是由光学可检测的代码反射或过滤的至少一个时，光学可检测的代码是可读的。 所述至少一个反射或滤波的光传播通过待检测的外部区域以读取光学可检测的代码。

公开(公告)号：US20100246005A1

公开(公告)日：2010-09-30

申请号：US11546027

申请日：2006-10-10

Applicant: John A. Moon ,  Martin A. Putnam

Inventor： John A. Moon ,  Martin A. Putnam

IPC: G02B5/18

CPC classification number: G03H1/02 ,  B01J2219/00459 ,  B01J2219/00463 ,  B01J2219/0047 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/00702 ,  B01L3/54 ,  G03H1/0272 ,  G03H2210/53 ,  G03H2230/10 ,  G03H2270/20 ,  G03H2270/24

Abstract: Microparticles 8 includes an optical substrate 10 having at least one diffraction grating 12 disposed therein. The grating 12 having a plurality of colocated pitches Λ which represent a unique identification digital code that is detected when illuminated by incident light 24. The incident light 24 may be directed transversely from the side of the substrate 10 with a narrow band (single wavelength) or multiple wavelength source, in which case the code is represented by a spatial distribution of light or a wavelength spectrum, respectively. The code may be digital binary or may be other numerical bases. The micro-particles 8 can provide a large number of unique codes, e.g., greater than 67 million codes, and can withstand harsh environments. The micro-particles 8 are functionalized by coating them with a material/substance of interest, which are then used to perform multiplexed experiments involving chemical processes, e.g., DNA testing and combinatorial chemistry.

Abstract translation: 微粒8包括其中设置有至少一个衍射光栅12的光学基板10。 光栅12具有多个共同定位的间距Λ，其表示当被入射光24照射时检测到的唯一识别数字码。入射光24可以从基底10的侧面以窄带（单波长）横向地引导， 或多个波长源，在这种情况下，代码分别由光或波长光谱的空间分布表示。 代码可以是数字二进制的，也可以是其他的数字基础。 微粒子8可以提供大量唯一的代码，例如大于6700万个代码，并且可以承受恶劣的环境。 通过用感兴趣的材料/物质涂覆微粒8来进行官能化，然后将其用于进行涉及化学过程（例如DNA测试和组合化学）的复合实验。

公开(公告)号：US07604173B2

公开(公告)日：2009-10-20

申请号：US11281910

申请日：2005-11-16

Applicant: Alan Kersey ,  John A. Moon ,  Martin A. Putnam

Inventor： Alan Kersey ,  John A. Moon ,  Martin A. Putnam

IPC: G06K7/10

CPC classification number: G06K7/10663

Abstract: A method and apparatus is provided for writing a code on an optical element, wherein the code is written on the optical element in the form of a holographic image of an n-dimensional code generated by an interference pattern between a reference beam and a signal beam reflected off a spatial light modulation device having the n-dimensional code configured thereon. The method includes steps of generating the interference pattern between the reference beam and the signal beam reflected off the spatial light modulation device having the n-dimensional code thereon; as well as writing the interference pattern on the optical element as a holographic image of the n-dimensional code.

Abstract translation: 提供了一种用于在光学元件上写入代码的方法和装置，其中以由参考光束和信号光束之间的干涉图案产生的n维码的全息图像的形式将代码写入光学元件 从其上配置有n维码的空间光调制装置反射。 该方法包括以下步骤：在参考光束与从其上具有n维码的空间光调制装置反射的信号光束之间产生干涉图案; 以及将干涉图案写入光学元件作为n维码的全息图像。