公开(公告)号：US4962028A

公开(公告)日：1990-10-09

申请号：US883604

申请日：1986-07-09

申请人： John R. Bedbrook ,  Caroline Dean ,  Pamela Dunsmuir

发明人： John R. Bedbrook ,  Caroline Dean ,  Pamela Dunsmuir

IPC分类号： C12N9/24 ,  C12N9/88 ,  C12N15/82

CPC分类号： C12Y302/01014 ,  C12N15/8216 ,  C12N15/8223 ,  C12N9/1007 ,  C12N9/2442 ,  C12N9/88 ,  C12Y201/01127

摘要： Promoter sequences from the gene from the small subunit of ribulose-1,5-bisphosphate carboxylase are disclosed. Expression cassettes containing a promoter sequence, a linker region, and a 3' fragment are also disclosed. The promotor sequences and expression cassettes are useful for expressing foreign genes to high levels in transformed plants.

摘要翻译： 公开了来自核酮糖-1,5-二磷酸羧化酶的小亚基的启动子序列。 还公开了含有启动子序列，接头区和3'片段的表达盒。 启动子序列和表达盒可用于在转化植物中将外源基因表达为高水平。

公开(公告)号：US07253274B2

公开(公告)日：2007-08-07

申请号：US10942711

申请日：2004-09-16

申请人： Caroline Dean ,  Anthony Gendall

发明人： Caroline Dean ,  Anthony Gendall

IPC分类号： C12N15/29

CPC分类号： C07K14/415 ,  C12N15/8241 ,  C12N15/8261 ,  Y02A40/146

摘要： An isolated nucleic acid obtainable from the VRN2 locus of a plant, which nucleic acid encodes a polypeptide which is capable of affecting one or more physical characteristics of a plant into which the nucleic acid is introduced, the physical characteristics being selected from vernalization response, flowering time, leaf size, and/or shape or shade avoidance response; alleles, fragment and derivatives thereof; polypeptides encoded by such nucleic acids; antibodies to such peptides.

摘要翻译： 可从植物的VRN2基因座获得的分离的核酸，其核酸编码能够影响引入其中的核酸的植物的一种或多种物理特性的多肽，物理特性选自春化反应，开花 时间，叶子大小和/或形状或阴影回避反应; 等位基因，片段和衍生物; 由该核酸编码的多肽; 对这些肽的抗体。

公开(公告)号：US06949694B2

公开(公告)日：2005-09-27

申请号：US10192985

申请日：2002-07-11

申请人： Gordon Grant Simpson ,  Caroline Dean ,  Paul Dijkwel

发明人： Gordon Grant Simpson ,  Caroline Dean ,  Paul Dijkwel

IPC分类号： C07K14/415 ,  C12N15/82 ,  C12Q1/68 ,  C12N15/29 ,  A01H5/00 ,  C12N5/04 ,  C12N5/10

CPC分类号： C12N15/827 ,  C07K14/415 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/158

摘要： The invention discloses nucleic acid encoding an FY polypeptide with the sequence shown in Annex 2. Also provided are vectors, host cells and plants. Methods of the invention include the use of nucleic acids to express or down-regulate FY in plants. The methods may be used to affect flowering time or juvenile phase length in plants.

摘要翻译： 本发明公开了编码具有附录2所示序列的FY多肽的核酸。还提供了载体，宿主细胞和植物。 本发明的方法包括使用核酸在植物中表达或下调FY。 该方法可用于影响植物中的开花时间或幼体期长度。

公开(公告)号：US08735173B2

公开(公告)日：2014-05-27

申请号：US13432387

申请日：2012-03-28

申请人： Peter John Meikle ,  John Joseph Hopwood ,  Douglas Alexander Brooks ,  Caroline Dean

发明人： Peter John Meikle ,  John Joseph Hopwood ,  Douglas Alexander Brooks ,  Caroline Dean

IPC分类号： G01N33/543

CPC分类号： G01N33/543 ,  A61K49/0058 ,  A61K49/0091 ,  G01N33/54313 ,  G01N33/582 ,  G01N33/6893

摘要： A novel protein profiling method of testing for Lysosomal Storage Diseases (“LSD”) using discovered normalized lysosomal fingerprint patterns. The fingerprint patterns reveal the health of lysosomal organelles, specific LSD, and clinical severity. Multiplexing bead technology for simultaneous screening of multiple LSD and normalizing measured enzyme activity or protein levels against other lysosomal proteins, enzymes, or enzyme activities. Compounds, reagents, and methods for identifying and quantifying multiple target enzymes and proteins.

摘要翻译： 一种使用发现的归一化溶酶体指纹图谱测定溶酶体贮积病（“LSD”）的新型蛋白质分析方法。 指纹图谱显示溶酶体细胞器的健康，特异性LSD和临床严重程度。 用于同时筛选多个LSD并使测定的酶活性或蛋白质水平与其他溶酶体蛋白质，酶或酶活性正常化的多重珠粒技术。 用于鉴定和定量多种靶酶和蛋白质的化合物，试剂和方法。

公开(公告)号：US20050060774A1

公开(公告)日：2005-03-17

申请号：US10894230

申请日：2004-07-19

申请人： Urban Johanson ,  Joanne West ,  Caroline Dean

发明人： Urban Johanson ,  Joanne West ,  Caroline Dean

IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/24

CPC分类号： C07K14/415 ,  A01K2217/05 ,  C12N15/827

摘要： Disclosed are isolated nucleic acids obtainable from the FRI locus of plants which encode polypeptides capable of specifically altering, particularly delaying, the flowering time of a plant into which the nucleic acid is introduced. One preferred embodiment is the FRI nucleotide sequence which encodes the polypeptide of FIG. 6 (see the sequence of FIG. 5, particularly bases 362-2188 thereof) or sequences degeneratively equivalent to these. Also provided are variant sequences (e.g. alleles, orthologues, derivatives) and complementary sequences, plus vectors, host cells, plants and associated processes of production and methods of use e.g. for influencing or affecting flowering time in a plant by expression or suppression of FRI or variant sequences.

摘要翻译： 公开了可从植物的FRI基因座获得的分离的核酸，其编码能够特异性改变，特别是延迟引入核酸的植物的开花时间的多肽。 一个优选的实施方案是编码图1的多肽的FRI核苷酸序列。 6（参见图5的序列，特别是其基因362-2188）或与其简并等价的序列。 还提供了变体序列（例如等位基因，直向同源物，衍生物）和互补序列，加上载体，宿主细胞，植物和相关生产过程以及使用方法，例如。 通过表达或抑制FRI或变体序列影响或影响植物中的开花时间。

公开(公告)号：US08173443B2

公开(公告)日：2012-05-08

申请号：US10551396

申请日：2004-03-31

申请人： Peter John Meikle ,  John Joseph Hopwood ,  Douglas Alexander Brooks ,  Caroline Dean

发明人： Peter John Meikle ,  John Joseph Hopwood ,  Douglas Alexander Brooks ,  Caroline Dean

IPC分类号： G01N33/543

CPC分类号： G01N33/543 ,  A61K49/0058 ,  A61K49/0091 ,  G01N33/54313 ,  G01N33/582 ,  G01N33/6893

摘要： A novel protein profiling method of testing for Lysosomal Storage Diseases (“LSD”) using discovered normalized lysosomal fingerprint patterns. The fingerprint patterns reveal the health of lysosomal organelles, specific LSD, and clinical severity Multiplexing bead technology for simultaneous screening of multiple LSD and normalizing measured enzyme activity or protein levels against other lysosomal proteins, enzymes, or enzyme activities. Compounds, reagents, and methods for identifying and quantifying multiple target enzymes and proteins.

摘要翻译： 一种使用发现的归一化溶酶体指纹图谱测定溶酶体贮积病（“LSD”）的新型蛋白质分析方法。 指纹图谱揭示了溶酶体细胞器，特异性LSD和临床严重性的复合珠粒技术，用于同时筛选多种LSD并使测定的酶活性或蛋白质水平与其他溶酶体蛋白质，酶或酶活性正常化。 用于鉴定和定量多种靶酶和蛋白质的化合物，试剂和方法。

公开(公告)号：US07045682B1

公开(公告)日：2006-05-16

申请号：US10088187

申请日：2000-09-13

申请人： Caroline Dean ,  Yaron Yakov Levy

发明人： Caroline Dean ,  Yaron Yakov Levy

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/04 ,  A01H5/00

CPC分类号： C12N15/827 ,  C07K14/415 ,  C12N15/8214 ,  C12N15/8238

摘要： Provided are isolated nucleic acid molecules which comprise VRN1 nucleotide sequences, which encode a polypeptide which is capable of specifically altering the vernalisation response of a plant into which the nucleic acid is introduced and expressed. Examples include cDNA and gDNA sequences (see e.g., Annex I). Also provided are variant molecules which may be derivatives or homologues (e.g., alleles, or paralogues such as RTV1), plus also complementary molecules. Corresponding polypeptides form a further part of the invention. The invention also provides methods and materials for preparing and using these molecules, e.g., in the production of plants having modified vernalisation characteristics. Also provided are methods for influencing and assessing the vernalisation phenotype of a plant.

摘要翻译： 提供了包含VRN1核苷酸序列的分离的核酸分子，其编码能够特异性改变引入和表达核酸的植物的春化反应的多肽。 实例包括cDNA和gDNA序列（参见例如附录I）。 还提供了可以是衍生物或同系物（例如等位基因或旁系同源物，例如RTV1）的变体分子，以及互补分子。 相应的多肽构成本发明的另一部分。 本发明还提供了用于制备和使用这些分子的方法和材料，例如在具有改性的春化特性的植物的生产中。 还提供了影响和评估植物的春化表型的方法。

公开(公告)号：US20050132446A1

公开(公告)日：2005-06-16

申请号：US10942711

申请日：2004-09-16

申请人： Caroline Dean ,  Anthony Gendall

发明人： Caroline Dean ,  Anthony Gendall

IPC分类号： C07K14/415 ,  C12N15/82 ,  C12Q1/68 ,  A01H1/00 ,  C12N5/04

CPC分类号： C07K14/415 ,  C12N15/8241 ,  C12N15/8261 ,  Y02A40/146

摘要： An isolated nucleic acid obtainable from the VRN2 locus of a plant, which nucleic acid encodes a polypeptide which is capable of affecting one or more physical characteristics of a plant into which the nucleic acid is introduced, the physical characteristics being selected from vernalization response, flowering time, leaf size, and/or shape or shade avoidance response; alleles, fragment and derivatives thereof; polypeptides encoded by such nucleic acids; antibodies to such peptides.

摘要翻译： 可从植物的VRN2基因座获得的分离的核酸，其核酸编码能够影响引入其中的核酸的植物的一种或多种物理特性的多肽，物理特性选自春化反应，开花 时间，叶子大小和/或形状或阴影回避反应; 等位基因，片段和衍生物; 由该核酸编码的多肽; 对这些肽的抗体。

公开(公告)号：US07615224B2

公开(公告)日：2009-11-10

申请号：US11291621

申请日：2005-12-01

申请人： Peter John Meikle ,  John Joseph Hopwood ,  Douglas Alexander Brooks ,  Caroline Dean

发明人： Peter John Meikle ,  John Joseph Hopwood ,  Douglas Alexander Brooks ,  Caroline Dean

IPC分类号： A61K39/395 ,  C07K16/00 ,  C07K17/00

CPC分类号： G01N33/54313 ,  C07K16/18 ,  C07K16/2896 ,  G01N2333/916 ,  G01N2333/924

摘要： Multiplexing bead technology is used for simultaneous screening of multiple LSD and normalizing measured enzyme activity or protein levels against other lysosomal proteins, enzymes, or enzyme activities. Diagnostic compositions include microspheres conjugated to purified antibodies that specifically bind LSD target antigens: saposin, LAMP-1, α-iduronidase, α-glucosidase, β-glucosidase, 2-sulphatase, 4-sulphatase, α-galactosidase, sphingomyelinase, 3-sulphatase or sulphamidase. The target antigens are naturally present in biological fluids or tissues of either LSD or non-LSD patients.

摘要翻译： 多重珠粒技术用于同时筛选多种LSD，并将测定的酶活性或蛋白质水平与其他溶酶体蛋白质，酶或酶活性标准化。 诊断组合物包括与纯化抗体结合的微球，其特异性结合LSD靶抗原：皂角蛋白，LAMP-1，α-艾杜糖酶，α-葡糖苷酶，β-葡糖苷酶，2-硫酸酯酶，4-硫酸酯酶，α-半乳糖苷酶，鞘磷脂酶，3-硫酸酯酶 或磺酰胺酶。 目标抗原天然存在于LSD或非LSD患者的生物流体或组织中。

公开(公告)号：US20070265432A1

公开(公告)日：2007-11-15

申请号：US11291621

申请日：2005-12-01

申请人： Peter Meikle ,  John Hopwood ,  Douglas Brooks ,  Caroline Dean

发明人： Peter Meikle ,  John Hopwood ,  Douglas Brooks ,  Caroline Dean

IPC分类号： C07K16/18

CPC分类号： G01N33/54313 ,  C07K16/18 ,  C07K16/2896 ,  G01N2333/916 ,  G01N2333/924

摘要： Multiplexing bead technology is used for simultaneous screening of multiple LSD and normalizing measured enzyme activity or protein levels against other lysosomal proteins, enzymes, or enzyme activities. Diagnostic compositions include microspheres conjugated to purified antibodies that specifically bind LSD target antigens: saposin, LAMP-1, α-iduronidase, α-glucosidase, β-glucosidase, 2-sulphatase, 4-sulphatase, α-galactosidase, sphingomyelinase, 3-sulphatase or sulphamidase. The target antigens are naturally present in biological fluids or tissues of either LSD or non-LSD patients.

摘要翻译： 多重珠粒技术用于同时筛选多种LSD，并将测定的酶活性或蛋白质水平与其他溶酶体蛋白质，酶或酶活性标准化。 诊断组合物包括与纯化抗体结合的微球，其特异性结合LSD靶抗原：皂角蛋白，LAMP-1，α-艾杜糖酶，α-葡糖苷酶，β-葡糖苷酶，2-硫酸酯酶，4-硫酸酯酶，α-半乳糖苷酶，鞘磷脂酶，3-硫酸酯酶 或磺酰胺酶。 目标抗原天然存在于LSD或非LSD患者的生物流体或组织中。