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    Stabilized primate lentivirus envelope glycoproteins
    5.
    发明授权
    Stabilized primate lentivirus envelope glycoproteins 失效
    稳定的灵长类动物慢病毒包膜糖蛋白

    公开(公告)号：US07048929B1

    公开(公告)日：2006-05-23

    申请号：US09446820

    申请日：1998-11-10

    申请人： Joseph G Sodroski ,  Richard T. Wyatt ,  Peter D. Kwong ,  Wayne A. Hendrickson ,  Michael Farzan

    发明人： Joseph G Sodroski ,  Richard T. Wyatt ,  Peter D. Kwong ,  Wayne A. Hendrickson ,  Michael Farzan

    IPC分类号： A61K39/21

    CPC分类号： C07K14/005 ,  C07K2319/00 ,  C12N2740/16122 ,  C12N2740/16134

    摘要： A modified polypeptide corresponding to an envelope glycoprotein of a primate lentivirus is described. The polypeptide has been modified from the wild-type structure so that it has cysteine amino acid residues introduced to create disulfide bonds, a cavity is filled with hydrophobic amino acids, a Proresidue is introduced at a defined turn structure of the protein, or the hydrophobicity is increased across the interface between different domains, while retaining the overall 3-dimensional structure of a discontinuous conserved epitope of the wild-type protein. Preferably, the polypeptide has more than one of those characteristics. Preferably, the primate lentivirus is HIV, and the protein is HIV-1 gp120.

    摘要翻译： 描述了与灵长类慢病毒的包膜糖蛋白相应的修饰的多肽。 已经从野生型结构修饰了多肽，使得其具有引入的半胱氨酸氨基酸残基以产生二硫键，空洞填充疏水性氨基酸，在定义的蛋白质结构引入前体，或疏水性 在不同结构域之间的界面上增加，同时保留野生型蛋白质的不连续保守表位的整体3维结构。 优选地，多肽具有多于一种特征。 优选地，灵长类动物慢病毒是HIV，蛋白质是HIV-1 gp120。

    Glycosylated modified primate lentivirus envelope polypeptides
    6.
    发明授权
    Glycosylated modified primate lentivirus envelope polypeptides 失效
    糖基化修饰的灵长类动物慢病毒包膜多肽

    公开(公告)号：US06908617B1

    公开(公告)日：2005-06-21

    申请号：US09446799

    申请日：1998-11-10

    申请人： Richard T. Wyatt ,  Joseph G. Sodroski ,  Peter D. Kwong ,  Wayne A. Hendrickson

    发明人： Richard T. Wyatt ,  Joseph G. Sodroski ,  Peter D. Kwong ,  Wayne A. Hendrickson

    IPC分类号： C07K19/00 ,  C12P21/00 ,  A61K19/00 ,  A36K36/16

    CPC分类号： C07K14/005 ,  C07K2319/21 ,  C07K2319/50 ,  C12N2740/16122

    摘要： A modified polypeptide corresponding to an envelope glycoprotein of a primate lentivirus is described. The polypeptide has been modified from the wild-type structure so that it has at least two of the glycosylation sites proximal to the CD4 binding site or chemokine receptor site altered so that the alteration prevents glycosylation at that site or where glycosylation sites distal to these sites have been derivatized with a molecular adjuvant, while retaining the overall 3-dimensional structure of a discontinuous conserved epitope of the wild-type protein. Preferably, the polypeptide has both changes. Preferably, the primate lentivirus is HIV, and the protein is HIV-1 gp 120.

    摘要翻译： 描述了与灵长类慢病毒的包膜糖蛋白相应的修饰的多肽。 已经从野生型结构修饰了多肽，使得其具有至少两个接近CD4结合位点或趋化因子受体位点的糖基化位点被改变，使得该改变防止在该位点处的糖基化，或者在这些位点远侧的糖基化位点 已经用分子佐剂衍生化，同时保留野生型蛋白质的不连续保守表位的整体3维结构。 优选地，多肽具有两个改变。 优选地，灵长类动物慢病毒是HIV，蛋白质是HIV-1gp120。

    Immunogenic proteoliposomes, and uses thereof
    7.
    发明授权
    Immunogenic proteoliposomes, and uses thereof 有权
    免疫原性蛋白脂质体及其用途

    公开(公告)号：US07276579B2

    公开(公告)日：2007-10-02

    申请号：US10450637

    申请日：2001-12-27

    申请人： Richard T. Wyatt ,  Joseph G. Sodroski ,  Tajib Mirzabekov ,  Christoph Grunder

    发明人： Richard T. Wyatt ,  Joseph G. Sodroski ,  Tajib Mirzabekov ,  Christoph Grunder

    IPC分类号： C07K2/00

    CPC分类号： A61K39/21 ,  A61K39/12 ,  A61K39/39 ,  A61K47/6911 ,  A61K2039/545 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/575 ,  C07K14/005 ,  C07K14/70503 ,  C07K14/7158 ,  C07K16/1063 ,  C12N2740/16122 ,  C12N2740/16134

    摘要： An immunogenic proteoliposome containing a transmembrane protein or oligomeric complexes containing such proteins, including viral envelope glycoproteins, in a lipid membrane around an elliptoid or spherical shape. The shape preferably also contains an attractant such as streptavidin or avidin and the lipid membrane contains a moiety that binds to the attractant such as biotin. The immunogenic transmembrane protein is bound to a ligand which is anchored in the shape. Methods for making the immunogenic proteoliposomes are provided uses of the proteoliposome are described, including their use as immunogens to elicit immune reaction, and their use in screening assays, including their use as antigens to screen antibody libraries, as well as for drug screening and the identification of ligands.

    摘要翻译： 含有跨膜蛋白或包含病毒包膜糖蛋白的蛋白质的寡聚复合物的免疫原性蛋白脂质体在围绕椭圆形或球形的脂质膜中。 该形状优选还含有引诱剂如链霉抗生物素蛋白或抗生物素蛋白，并且脂质膜含有结合引诱剂如生物素的部分。 免疫原性跨膜蛋白与锚定在该形状的配体结合。 提供了制备免疫原性蛋白脂质体的方法，描述了脂蛋白体的用途，包括其作为免疫原用于引发免疫反应的用途，以及它们在筛选测定中的应用，包括其用作抗体筛选抗体文库以及用于药物筛选和 鉴定配体。