公开(公告)号：US4330440A

公开(公告)日：1982-05-18

申请号：US128847

申请日：1980-03-10

申请人： John S. Ayers ,  Geoffrey S. Bethell ,  William S. Hancock ,  Milton T. W. Hearn

发明人： John S. Ayers ,  Geoffrey S. Bethell ,  William S. Hancock ,  Milton T. W. Hearn

IPC分类号： C08B15/06 ,  C08B37/00 ,  C12N11/10 ,  G01N33/543 ,  G01N33/548 ,  C08L1/00

CPC分类号： G01N33/548 ,  C08B15/06 ,  C08B37/0039 ,  C12N11/10 ,  G01N33/54353

摘要： The invention relates to the carbonylation of cross linked substituted and unsubstituted polysaccharides, their copolymers with macroporous synthetic polymers, macroporous synthetic polymers and rigid supports with pendant hydroxyalkyl groups. The carbonylated product can be used to prepare an affinity chromatography matrix which remains an uncharged species at varying pH's. The carbonylated product is also useful for the preparation of other compounds: Typical polysaccharides are agarose, starch, dextran, cellulose and regenerated cellulose, typical macroporous synthetic polymers are acrylamides, acrylates and methacrylates, typical rigid supports are silica beads coated with hydroxy alkyl groups and typical cabonylating agents are N,N'-carbonyl diimidazole; N,N'carbonyl di-1,2,3-benzotriazole; and N,N'-carbonyl di-1,2,4-triazole.

摘要翻译： 本发明涉及交联的取代和未取代的多糖，它们与大孔合成聚合物的共聚物，大孔合成聚合物和具有侧羟基烷基的刚性载体的羰基化。 羰基化产物可用于制备在不同pH值下保持不带电荷的物质的亲和层析基质。 羰基化产物也可用于制备其他化合物：典型的多糖是琼脂糖，淀粉，葡聚糖，纤维素和再生纤维素，典型的大孔合成聚合物是丙烯酰胺，丙烯酸酯和甲基丙烯酸酯，典型的刚性载体是用羟基烷基涂覆的二氧化硅珠， 典型的卡罗胺化剂是N，N'-羰基二咪唑; N，N'-羰基二-1,2,3-苯并三唑; 和N，N'-羰基二-1,2,4-三唑。

公开(公告)号：US4224439A

公开(公告)日：1980-09-23

申请号：US874628

申请日：1978-02-02

申请人： John S. Ayers ,  Geoffrey S. Bethell ,  William S. Hancock ,  Milton T. W. Hearn

发明人： John S. Ayers ,  Geoffrey S. Bethell ,  William S. Hancock ,  Milton T. W. Hearn

IPC分类号： C08B15/06 ,  C08B37/00 ,  C12N11/10 ,  G01N33/543 ,  G01N33/548 ,  C08B3/00

CPC分类号： C12N11/10 ,  C08B15/06 ,  C08B37/0039 ,  G01N33/54353 ,  G01N33/548 ,  Y10S530/813

摘要： Carbonylation of a polysaccharide and the product of that carbonylation. The carbonylated product can be used to prepare an affinity chromatography matrix which is an uncharged species of varying pH's. The carbonylated product is also useful for the preparation of other compounds. Typical polysaccharides are agarose, starch, dextran, cellulose and regenerated cellulose and typical carbonylating agents are N,N'-carbonyl diimidazole; N,N' carbonyl di-1,2,3-benzotriazole; and N,N'-carbonyl di-1,2,4-triazole.

摘要翻译： 多糖的羰基化和该羰基化的产物。 羰基化产物可用于制备不同pH值的不带电荷的亲和层析基质。 羰基化产物也可用于制备其它化合物。 典型的多糖是琼脂糖，淀粉，葡聚糖，纤维素和再生纤维素，典型的羰基化剂是N，N'-羰基二咪唑; N，N'羰基二-1,2,3-苯并三唑; 和N，N'-羰基二-1,2,4-三唑。

公开(公告)号：US4603010A

公开(公告)日：1986-07-29

申请号：US736306

申请日：1985-05-21

申请人： John S. Ayers ,  William S. Hancock ,  David R. K. Harding

发明人： John S. Ayers ,  William S. Hancock ,  David R. K. Harding

IPC分类号： C07K14/00 ,  A61K35/14 ,  A61K35/16 ,  A61K38/16 ,  A61M1/36 ,  B01J39/04 ,  B01J47/00 ,  C07K15/16

CPC分类号： B01J47/003 ,  A61K35/16 ,  B01J39/043

摘要： A process is described for removing low density and very low density lipoproteins from blood plasma or serum and recovering the plasma or serum in a physiologically acceptable form. The method involves in a preferred embodiment passing plasma or serum through a cationic ion exchanger equilibrated with a physiologically acceptable saline solution. The ion exchanger is a water insoluble hydrophilic, water swellable cross-linked regenerated or microgranular cellulose matrix substituted with hydroxy C.sub.2 -C.sub.4 alkyl group. The ion exchanger capacity is provided by sulphate groups substituted with from 2 to 6 meq/g. The take up of low density lipoproteins is enhanced by recycling the plasma through the column.

摘要翻译： 描述了从血浆或血清中除去低密度和极低密度脂蛋白的方法，并以生理上可接受的形式回收血浆或血清。 该方法包括在优选实施方案中使血浆或血清通过用生理学上可接受的盐水溶液平衡的阳离子离子交换剂。 离子交换剂是由羟基C 2 -C 4烷基取代的水不溶性亲水性，水溶胀性交联的再生或微粒状纤维素基质。 离子交换容量由2〜6meq / g取代的硫酸盐基团提供。 低密度脂蛋白的吸收通过将血浆循环通过柱来增强。

公开(公告)号：US4178439A

公开(公告)日：1979-12-11

申请号：US773269

申请日：1977-03-01

申请人： John S. Ayers ,  David R. Husbands

发明人： John S. Ayers ,  David R. Husbands

IPC分类号： C08B15/10 ,  B01J20/281 ,  B01J39/22 ,  C08B5/14 ,  C08B15/00 ,  C08B37/00 ,  G01N30/88 ,  G01N33/68 ,  C08B7/00 ,  C08B37/02 ,  C08B37/04

CPC分类号： B01J39/22 ,  C08B15/005 ,  C08B37/00 ,  C08B5/14

摘要： The invention relates to a novel sulphated cationic ion exchanger and to a method for preparation thereof. In addition it relates to a method of improving the capacity of an exchange matrix to receive sulphated groups. Although the general attraction of sulphate groups to proteins is known we have found that sulphated ion exchangers selectively bind lipoproteins in blood serum or plasma under suitable conditions. We have also discovered that when hydroxyalkyl groups are introduced into the ion exchanger matrix its capacity for taking up sulphate groups is unexpectedly increased. A method of use of the ion exchanger is also described.

摘要翻译： 本发明涉及一种新型的硫酸化阳离子交换剂及其制备方法。 此外，它涉及提高交换基质以接收硫酸化基团的能力的方法。 虽然硫酸盐基团对蛋白质的一般吸引力是已知的，但我们发现硫酸化离子交换剂在合适的条件下选择性地结合血清或血浆中的脂蛋白。 我们还发现，当将羟基烷基引入离子交换剂基质时，其吸收硫酸盐基团的能力意外地增加。 还描述了使用离子交换剂的方法。

公开(公告)号：US4175183A

公开(公告)日：1979-11-20

申请号：US909165

申请日：1978-05-24

申请人： John S. Ayers

发明人： John S. Ayers

IPC分类号： B01J20/22 ,  B01J39/22 ,  B01J41/16 ,  C08B11/08 ,  C08B15/00 ,  C08B11/193 ,  C08B15/10

CPC分类号： C08B11/08 ,  B01J20/22 ,  B01J39/22 ,  B01J41/16 ,  C08B15/005

摘要： The invention relates to a novel water insoluble hydroxyalkylated cross-linked regenerated cellulose and a method for its preparation. The product is characterized by having outstanding swelling characteristics and high chemical reactivity. A method of preparing the product using near stoichiometric proportions of reagents is described. Use of the product directly in gel chromatography and as an intermediate in the preparation of ion exchangers is described.

摘要翻译： 本发明涉及一种新型的水不溶性羟烷基化交联再生纤维素及其制备方法。 该产品具有突出的溶胀特性和高的化学反应性。 描述了使用接近化学计量比的试剂制备产物的方法。 描述了直接在凝胶色谱中使用该产物和作为制备离子交换剂的中间体。