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公开(公告)号：US20090075333A1
公开(公告)日：2009-03-19
申请号：US12064332
申请日：2006-08-18
摘要： Reduced genome strains of E. coli MGl 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
摘要翻译： 描述了大肠杆菌MG155的减少的基因组菌株。 在各种实施方案中，与亲本菌株和市售菌株相比，菌株具有相同或改善的生长速率，转化效率，蛋白质表达，DNA产生，DNA产量和/或DNA质量的一种或多种。
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公开(公告)号：US4920686A
公开(公告)日：1990-05-01
申请号：US307099
申请日：1989-02-07
IPC分类号： A01K85/01
CPC分类号： A01K85/01
摘要： A fishing lure incorporating a pocket dimensioned to hold a sinker and/or rattle within the lure body includes an easy access slit formed in the lure material to allow ready exchange of sinker/rattle combinations.
摘要翻译： 钓鱼诱饵包含尺寸适于在诱饵体内保持沉降片和/或拨浪鼓的口袋，包括形成在诱饵材料中的容易进入狭缝，以允许即将更换沉降片/拨浪鼓组合。
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公开(公告)号：US4873783A
公开(公告)日：1989-10-17
申请号：US307823
申请日：1989-02-07
IPC分类号： A01K85/00
CPC分类号： A01K85/00
摘要： A fishing lure including a thin walled pouch for enclosing that portion of a fishing hook which normally extends from a lure body when the lure is threaded on a hook.
摘要翻译： 捕鱼诱饵包括一个薄壁袋，用于封闭钓鱼钩的那部分，当诱饵穿在钩上时，钓鱼钩通常从诱饵体延伸。
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公开(公告)号：US08765408B2
公开(公告)日：2014-07-01
申请号：US13363848
申请日：2012-02-01
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译： 本发明提供了一种具有遗传工程化以小于其天然亲本菌株的基因组的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。
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公开(公告)号：US20130011874A1
公开(公告)日：2013-01-10
申请号：US13445672
申请日：2012-04-12
摘要： Reduced genome strains of E. coli MG1 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
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公开(公告)号：US20100320129A1
公开(公告)日：2010-12-23
申请号：US12854420
申请日：2010-08-11
申请人： John W. Campbell
发明人： John W. Campbell
摘要： A facility for treating solid and liquid waste includes a receiving station, a screening station, a preprocessing station, an alkali mixing station, a press station and a processing station. The screening station is connected to an inlet capable of receiving liquid and solid waste and includes a screen for capturing at least some of the solid waste. The preprocessing station is in fluid communication with the screening station, and includes a degrit chamber for settling out an additional amount of the solids. The alkali mixing station includes an alkali for mixing with the waste to stabilize the waste at a predetermined pH level. The press station receives the stabilized waste and includes a press for separating out an additional portion of the solids. The processing station includes at least one aerobic microorganism generating unit for converting nitrites into nitrogen gas and consuming carbon-based waste material.
摘要翻译： 用于处理固体和液体废物的设备包括接收站，筛选站，预处理站，碱混合站，压力站和处理站。 筛选站连接到能够接收液体和固体废物的入口，并且包括用于捕获至少一些固体废物的筛网。 预处理站与筛选站流体连通，并且包括用于沉淀附加量的固体的除渣室。 碱混合站包括用于与废物混合的碱，以将废物稳定在预定的pH水平。 压力站接收稳定的废物，并包括用于分离附加部分固体的压力机。 处理站包括至少一个用于将亚硝酸盐转化成氮气并消耗碳基废料的好氧微生物产生单元。
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公开(公告)号：US07794597B2
公开(公告)日：2010-09-14
申请号：US12142582
申请日：2008-06-19
申请人： John W. Campbell
发明人： John W. Campbell
IPC分类号： C02F3/00
摘要： A facility for treating solid and liquid waste includes a receiving station, a screening station, a preprocessing station, a press station and a processing station. The screening station is connected to an inlet capable of receiving liquid and solid waste and includes a screen for capturing at least some of the solid waste. The preprocessing station is in fluid communication with the screening station, and includes a degrit chamber for settling out an additional amount of the solids. The press station included at least one alkali mixing tank and a screw press for separating out an additional portion of the solids. The processing station includes at least one aerobic microorganism generating unit for converting nitrites into nitrogen gas and consuming carbon-based waste material.
摘要翻译： 用于处理固体和液体废物的设施包括接收站，筛选站，预处理站，压力站和处理站。 筛选站连接到能够接收液体和固体废物的入口，并且包括用于捕获至少一些固体废物的筛网。 预处理站与筛选站流体连通，并且包括用于沉淀附加量的固体的除渣室。 压力站包括至少一个碱性混合罐和螺旋压力机，用于分离出附加部分的固体。 处理站包括至少一个用于将亚硝酸盐转化成氮气并消耗碳基废料的好氧微生物产生单元。
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公开(公告)号：US20080314828A1
公开(公告)日：2008-12-25
申请号：US12142582
申请日：2008-06-19
申请人： John W. Campbell
发明人： John W. Campbell
IPC分类号： C02F3/02
摘要： A facility for treating solid and liquid waste includes a receiving station, a screening station, a preprocessing station, a press station and a processing station. The screening station is connected to an inlet capable of receiving liquid and solid waste and includes a screen for capturing at least some of the solid waste. The preprocessing station is in fluid communication with the screening station, and includes a degrit chamber for settling out an additional amount of the solids. The press station included at least one alkali mixing tank and a screw press for separating out an additional portion of the solids. The processing station includes at least one aerobic microorganism generating unit for converting nitrites into nitrogen gas and consuming carbon-based waste material.
摘要翻译： 用于处理固体和液体废物的设施包括接收站，筛选站，预处理站，压力站和处理站。 筛选站连接到能够接收液体和固体废物的入口，并且包括用于捕获至少一些固体废物的筛网。 预处理站与筛选站流体连通，并且包括用于沉淀附加量的固体的除渣室。 压力站包括至少一个碱性混合罐和螺旋压力机，用于分离出附加部分的固体。 处理站包括至少一个用于将亚硝酸盐转化成氮气并消耗碳基废料的好氧微生物产生单元。
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公开(公告)号：US4891508A
公开(公告)日：1990-01-02
申请号：US213405
申请日：1988-06-30
申请人： John W. Campbell
发明人： John W. Campbell
CPC分类号： G01V8/20
摘要： The precision infrared position detector apparatus uses an information display device that has a substantially cylindrical shaped screen to limit curvature of the screen to a single dimension. In addition, to compensate for this curvature, a flexible printed circuit board is used to align the row of infrared light sources and detectors along the same curvature as the screen of the information display device. A first flexible printed circuit board is equipped with a single row of infrared light sources while a second flexible printed circuit board is equipped with a signal row of corresponding infrared light detectors. A frame member is used to bend the flexible printed circuit board into a substantially cylindrical shape that matches the curvature of the screen of the information display device. In this manner, the first and second flexible printed circuit boards are placed at opposite ends of the rectangular shaped screen of the information display device to thereby transmit a plurality of parallel beams of infrared light, each of which is a predetermined distance above the screen of the information display device even though the screen of the information display device curves in the shape of a cylinder.
摘要翻译： 精密红外位置检测装置使用具有大致圆筒形的屏幕的信息显示装置，以将屏幕的曲率限制为单个尺寸。 此外，为了补偿该曲率，使用柔性印刷电路板将红外光源和检测器行与信息显示装置的屏幕相同的曲率对准。 第一柔性印刷电路板配备有单排红外光源，而第二柔性印刷电路板配备有相应红外光检测器的信号行。 框架构件用于将柔性印刷电路板弯曲成与信息显示装置的屏幕的曲率相匹配的大致圆柱形形状。 以这种方式，第一和第二柔性印刷电路板被放置在信息显示装置的矩形屏幕的相对端，从而传输多个平行的红外光束，每个平行的红外光束是屏幕上方的预定距离 信息显示装置即使信息显示装置的画面呈圆柱形的形状。
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公开(公告)号：US20120219994A1
公开(公告)日：2012-08-30
申请号：US13363848
申请日：2012-02-01
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译： 本发明提供了一种具有遗传工程化以小于其天然亲本菌株基因组的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。
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