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公开(公告)号：US07264929B2
公开(公告)日：2007-09-04
申请号：US10299180
申请日：2002-11-19
申请人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
发明人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
CPC分类号： G01N21/6452 , B01L3/5027 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B01L2300/0893 , B82Y15/00 , B82Y30/00 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , G01N21/0303 , G01N21/7703 , C12Q2565/519 , C12Q2533/101 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.
摘要翻译： 本文公开了用于测序核酸的方法和装置。 这些方法允许非常大量的独立测序反应并行排列，允许非常大数量（> 10,000）不同寡核苷酸的同时测序。
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公开(公告)号：US07335762B2
公开(公告)日：2008-02-26
申请号：US10222592
申请日：2002-08-15
申请人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo , Michael P. Weiner
发明人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo , Michael P. Weiner
CPC分类号： G01N21/6452 , B01L3/5027 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B01L2300/0893 , B82Y15/00 , B82Y30/00 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , G01N21/0303 , G01N21/7703 , C12Q2565/519 , C12Q2533/101 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.
摘要翻译： 本文公开了用于测序核酸的方法和装置。 这些方法允许非常大量的独立测序反应并行排列，允许非常大数量（> 10,000）不同寡核苷酸的同时测序。
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公开(公告)号：US07244559B2
公开(公告)日：2007-07-17
申请号：US09814338
申请日：2001-03-21
申请人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
发明人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
CPC分类号： G01N21/6452 , B01L3/5027 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B01L2300/0893 , B82Y15/00 , B82Y30/00 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , G01N21/0303 , G01N21/7703 , C12Q2565/519 , C12Q2533/101 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.
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公开(公告)号：US07211390B2
公开(公告)日：2007-05-01
申请号：US10788529
申请日：2004-02-26
申请人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
发明人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
CPC分类号： C12Q1/6837 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B82Y30/00 , C12Q1/6827 , C12Q1/6869 , C12Q1/6874 , G01N21/6452 , G01N2201/08 , G01N2201/0846 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence byproduct such as pyrophosphate.
摘要翻译： 本文公开了用于测序核酸的方法和装置。 一方面，该方法包括将多个环状核酸分子退火至与固体支持物连接的多个锚定引物，并扩增与靶核酸退火的环状核酸分子群体的那些成员，然后测序 通过检测序列副产物如焦磷酸盐的存在来扩增分子。
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公开(公告)号：US20070092872A1
公开(公告)日：2007-04-26
申请号：US10222298
申请日：2002-08-15
申请人： Jonathan Rothberg , Joel Bader , Scott Dewell , Keith McDade , John Simpson , Jan Berka , Christopher Colangelo , Michael Weiner
发明人： Jonathan Rothberg , Joel Bader , Scott Dewell , Keith McDade , John Simpson , Jan Berka , Christopher Colangelo , Michael Weiner
CPC分类号： G01N21/6452 , B01L3/5027 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B01L2300/0893 , B82Y15/00 , B82Y30/00 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , G01N21/0303 , G01N21/7703 , C12Q2565/519 , C12Q2533/101 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.
摘要翻译： 本文公开了用于测序核酸的方法和装置。 这些方法允许非常大量的独立测序反应并行排列，允许非常大数量（> 10,000）不同寡核苷酸的同时测序。
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6.发明申请System and Method for Correcting Primer Extension Errors in Nucleic Acid Sequence Data 有权用于校正核酸序列数据中引物延伸错误的系统和方法公开(公告)号：US20100192032A1
公开(公告)日：2010-07-29
申请号：US12224065
申请日：2007-02-15
申请人： Yi-Ju Chen , Keith McDade , John Simpson
发明人： Yi-Ju Chen , Keith McDade , John Simpson
CPC分类号： C12Q1/6848 , C12Q1/6869 , G06F19/22 , G06F19/24 , C12Q2537/165
摘要： An embodiment of method for correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copy of a template molecule is described that comprises (a) detecting a signal generated in response to an incorporation of one or more nucleotides in a sequencing reaction; (b) generating a value for the signal; and (c) correcting the value for the phasic synchrony error using a first parameter and a second carry forward parameter.
摘要翻译： 描述了用于校正与基本相同的模板分子拷贝生成的序列数据的相位同步相关的误差的方法的实施方案，其包括（a）检测响应于一个或多个核苷酸的引入产生的信号 测序反应; （b）产生该信号的值; 和（c）使用第一参数和第二进位参数来校正所述相位同步误差的值。
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7.发明授权System and method for correcting primer extension errors in nucleic acid sequence data 有权用于校正核酸序列数据中引物延伸误差的系统和方法公开(公告)号：US09587274B2
公开(公告)日：2017-03-07
申请号：US13608841
申请日：2012-09-10
申请人： Yi-Ju Chen , Keith McDade , John Simpson
发明人： Yi-Ju Chen , Keith McDade , John Simpson
CPC分类号： C12Q1/6848 , C12Q1/6869 , G06F19/22 , G06F19/24 , C12Q2537/165
摘要： An embodiment of method for correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copies of a template molecule is described that comprises (a) detecting a signal generated in response to an incorporation of one or more nucleotides in a sequencing reaction; (b) generating a value for the signal; and (c) correcting the value for the phasic synchrony error using a first parameter and a second carry forward parameter.
摘要翻译： 描述了用于校正与从模板分子的基本相同拷贝的群体产生的序列数据的相位同步相关联的错误的方法的实施方案，其包括（a）检测响应于在一个或多个核苷酸中的一个或多个核苷酸的引入而产生的信号 测序反应; （b）产生该信号的值; 和（c）使用第一参数和第二进位参数来校正所述相位同步误差的值。
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8.发明申请System and Method for Correcting Primer Extension Errors in Nucleic Acid Sequence Data 有权用于校正核酸序列数据中引物延伸错误的系统和方法公开(公告)号：US20130054171A1
公开(公告)日：2013-02-28
申请号：US13608841
申请日：2012-09-10
申请人： Yi-Ju Chen , Keith McDade , John Simpson
发明人： Yi-Ju Chen , Keith McDade , John Simpson
IPC分类号： G06F19/18
CPC分类号： C12Q1/6848 , C12Q1/6869 , G06F19/22 , G06F19/24 , C12Q2537/165
摘要： An embodiment of method for correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copies of a template molecule is described that comprises (a) detecting a signal generated in response to an incorporation of one or more nucleotides in a sequencing reaction; (b) generating a value for the signal; and (c) correcting the value for the phasic synchrony error using a first parameter and a second carry forward parameter.
摘要翻译： 描述了用于校正与从模板分子的基本相同拷贝的群体产生的序列数据的相位同步相关联的错误的方法的实施方案，其包括（a）检测响应于在一个或多个核苷酸中的并入产生的信号 测序反应; （b）产生该信号的值; 和（c）使用第一参数和第二进位参数来校正所述相位同步误差的值。
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9.发明授权System and method for correcting primer extension errors in nucleic acid sequence data 有权用于校正核酸序列数据中引物延伸误差的系统和方法公开(公告)号：US08301394B2
公开(公告)日：2012-10-30
申请号：US12224065
申请日：2007-02-15
申请人： Yi-Ju Chen , Keith McDade , John Simpson
发明人： Yi-Ju Chen , Keith McDade , John Simpson
CPC分类号： C12Q1/6848 , C12Q1/6869 , G06F19/22 , G06F19/24 , C12Q2537/165
摘要： An embodiment of method for correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copies of a template molecule is described that comprises (a) detecting a signal generated in response to an incorporation of one or more nucleotides in a sequencing reaction; (b) generating a value for the signal; and (c) correcting the value for the phasic synchrony error using a first parameter and a second carry forward parameter.
摘要翻译： 描述了用于校正与从模板分子的基本相同拷贝的群体产生的序列数据的相位同步相关联的错误的方法的实施方案，其包括（a）检测响应于在一个或多个核苷酸中的一个或多个核苷酸的引入而产生的信号 测序反应; （b）产生该信号的值; 和（c）使用第一参数和第二进位参数来校正所述相位同步误差的值。
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