公开(公告)号：US09790534B2

公开(公告)日：2017-10-17

申请号：US14146367

申请日：2014-01-02

申请人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

发明人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

IPC分类号： C12Q1/04 ,  G01N33/68 ,  H01J49/00

CPC分类号： C12Q1/04 ,  G01N33/6848 ,  H01J49/00

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

公开(公告)号：US10415075B2

公开(公告)日：2019-09-17

申请号：US12589936

申请日：2009-10-30

申请人： Jones Hyman ,  Bradford Clay ,  John Walsh ,  Thurman Thorpe

发明人： Jones Hyman ,  Bradford Clay ,  John Walsh ,  Thurman Thorpe

IPC分类号： C12Q1/04 ,  G01N33/68 ,  H01J49/00

摘要： A method is described to separate, characterize and/or identify microorganisms in a test sample. Said method comprises the steps of (i) lysing non-microorganism cells that might be present in the sample, (ii) separating the microorganisms as a pellet in the sample post-lysing non-microorganism cells, (iii) interrogating the pellet containing microorganisms by mass spectrometry to produce mass spectra of microorganisms, and (iv) characterizing and/or identifying microorganisms in the sample. The method may be used in characterizing/identifying microorganisms in complex samples, e.g., blood containing culture media.

公开(公告)号：US20140186881A1

公开(公告)日：2014-07-03

申请号：US14200662

申请日：2014-03-07

申请人： John Walsh ,  Jones Hyman ,  Bradford Clay ,  Thurman Thorpe

发明人： John Walsh ,  Jones Hyman ,  Bradford Clay ,  Thurman Thorpe

IPC分类号： C12Q1/04 ,  C12Q1/10

CPC分类号： C12Q1/04 ,  C12Q1/10 ,  G01N21/31 ,  G01N21/47 ,  G01N21/64 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6421

摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample whereby the method may be accomplished non-invasively by utilizing a time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle, that may be present in the sample.

摘要翻译： 本发明提供了一种用于监测，检测和/或表征可能存在于样品中的生物颗粒的方法和系统，其中所述方法可以非侵入地通过利用时间依赖的光谱技术来获得至少两个测量 的生长组合物，其包含样品并将所述测量结果与可能存在于样品中的生物颗粒的检测和/或表征相关联。

公开(公告)号：US10435733B2

公开(公告)日：2019-10-08

申请号：US14200662

申请日：2014-03-07

申请人： John Walsh ,  Jones Hyman ,  Bradford Clay ,  Thurman Thorpe

发明人： John Walsh ,  Jones Hyman ,  Bradford Clay ,  Thurman Thorpe

IPC分类号： C12Q1/04 ,  G01N21/31 ,  G01N21/64 ,  C12Q1/10 ,  G01N21/47

摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample whereby the method may be accomplished non-invasively by utilizing a time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle, that may be present in the sample.

公开(公告)号：US20100120085A1

公开(公告)日：2010-05-13

申请号：US12589936

申请日：2009-10-30

申请人： Jones Hyman ,  Bradford Clay ,  John Walsh ,  Thurman Thorpe

发明人： Jones Hyman ,  Bradford Clay ,  John Walsh ,  Thurman Thorpe

IPC分类号： C12Q1/04 ,  C12Q1/02

CPC分类号： C12Q1/04 ,  G01N33/6848 ,  H01J49/00

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for interrogation of the separated microorganism sample by mass spectrometry to produce a mass spectrum of the microorganism and characterizing and/or identifying the microorganism in the sample using the mass spectrum obtained.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供了通过质谱法对分离的微生物样品进行询问以产生微生物的质谱，并使用所获得的质谱来表征和/或鉴定样品中的微生物。

公开(公告)号：US20140186879A1

公开(公告)日：2014-07-03

申请号：US14146367

申请日：2014-01-02

申请人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

发明人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  G01N33/6848 ,  H01J49/00

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

公开(公告)号：US20100136609A1

公开(公告)日：2010-06-03

申请号：US12589976

申请日：2009-10-30

申请人： Bradford Clay ,  Jones Hyman ,  John Walsh ,  Thurman Thorpe ,  Christopher Ronsick

发明人： Bradford Clay ,  Jones Hyman ,  John Walsh ,  Thurman Thorpe ,  Christopher Ronsick

IPC分类号： C12Q1/04 ,  C12Q1/02

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for Raman spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said Raman spectroscopic measurements.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供分离的微生物样品的拉曼光谱询问以产生微生物的测量，并使用所述拉曼光谱测量来表征和/或鉴定样品中的微生物。

公开(公告)号：US20100129814A1

公开(公告)日：2010-05-27

申请号：US12589985

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/68 ,  G01N33/567 ,  C12Q1/37 ,  C12Q1/04

CPC分类号： C12Q1/24 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/6816 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for the use of one or more identifier agents and interrogating the microorganism sample and/or said one or more identifier agents to produce measurements which characterizing and/or identifying the microorganism based on the produced measurements and/or the presence or absence of the identifier agent or a metabolized form of the identifier agent in the microorganism sample.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供一种或多种识别剂的使用，并且询问微生物样品和/或所述一种或多种标识物以产生基于产生的测量值和/或存在或不存在来表征和/或鉴定微生物的测量结果 的识别剂或微生物样品中识别剂的代谢形式。

公开(公告)号：US10167494B2

公开(公告)日：2019-01-01

申请号：US12589968

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/00 ,  C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/3577

摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.

公开(公告)号：US10059975B2

公开(公告)日：2018-08-28

申请号：US12589929

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/359

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.