公开(公告)号：US08735091B2

公开(公告)日：2014-05-27

申请号：US13828119

申请日：2013-03-14

申请人： Jones M. Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

发明人： Jones M. Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

IPC分类号： C12Q1/24

CPC分类号： G01N1/4077 ,  C12Q1/24 ,  G01N33/5695 ,  G01N33/6851 ,  G01N2333/35 ,  G01N2333/355 ,  G01N2560/00

摘要： The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g., mycobacteria or nocardia), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.

摘要翻译： 本发明涉及一种灭活和/或提取酸性抗菌（例如分枝杆菌或诺卡氏菌）的方法，该方法包括以下顺序步骤：（a）获得已知含有或可含有酸的测试样品 将试样悬浮在含有乙醇和珠粒的容器中; （b）珠子打浆和/或涡旋容器以分解块和/或破坏容器中的耐酸细菌细胞; 和（c）随后在室温下将悬浮液温育至少约3分钟以灭活测试样品中所含的任何耐酸细菌。 根据本发明，随后通过离心将测试样品沉淀，用甲酸重悬浮，加入乙腈，并进行质谱鉴定和/或鉴定耐酸细菌。

公开(公告)号：US20130309714A1

公开(公告)日：2013-11-21

申请号：US13828119

申请日：2013-03-14

申请人： Jones Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

发明人： Jones Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

IPC分类号： G01N1/40

CPC分类号： G01N1/4077 ,  C12Q1/24 ,  G01N33/5695 ,  G01N33/6851 ,  G01N2333/35 ,  G01N2333/355 ,  G01N2560/00

摘要： The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g., mycobacteria or nocardia), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.

摘要翻译： 本发明涉及一种灭活和/或提取酸性抗菌（例如分枝杆菌或诺卡氏菌）的方法，该方法包括以下顺序步骤：（a）获得已知含有或可含有酸的测试样品 将试样悬浮在含有乙醇和珠粒的容器中; （b）珠子打浆和/或涡旋容器以分解块和/或破坏容器中的耐酸细菌细胞; 和（c）随后在室温下将悬浮液温育至少约3分钟以灭活测试样品中所含的任何耐酸细菌。 根据本发明，随后通过离心将测试样品沉淀，用甲酸重悬浮，加入乙腈，并进行质谱鉴定和/或鉴定耐酸细菌。

公开(公告)号：US10167494B2

公开(公告)日：2019-01-01

申请号：US12589968

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/00 ,  C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/3577

摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.

公开(公告)号：US10059975B2

公开(公告)日：2018-08-28

申请号：US12589929

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/359

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.

公开(公告)号：US20100129858A1

公开(公告)日：2010-05-27

申请号：US12589952

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848 ,  Y02A50/55

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

公开(公告)号：US20100129857A1

公开(公告)日：2010-05-27

申请号：US12589929

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/04 ,  C12N1/00

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供了在单个系统内原位分离，表征和/或鉴定微生物的方法。

公开(公告)号：US08652800B2

公开(公告)日：2014-02-18

申请号：US12589952

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848 ,  Y02A50/55

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

公开(公告)号：US09128058B2

公开(公告)日：2015-09-08

申请号：US12589985

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64

CPC分类号： C12Q1/24 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/6816 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for the use of one or more identifier agents and interrogating the microorganism sample and/or said one or more identifier agents to produce measurements which characterizing and/or identifying the microorganism based on the produced measurements and/or the presence or absence of the identifier agent or a metabolized form of the identifier agent in the microorganism sample.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供一种或多种识别剂的使用，并且询问微生物样品和/或所述一种或多种标识物以产生基于产生的测量值和/或存在或不存在来表征和/或鉴定微生物的测量结果 的识别剂或微生物样品中识别剂的代谢形式。

公开(公告)号：US20110033847A1

公开(公告)日：2011-02-10

申请号：US12653527

申请日：2009-12-15

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe

IPC分类号： C12Q1/04 ,  C12Q1/68 ,  G01N33/566

CPC分类号： C12Q1/04

摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

摘要翻译： 本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。 所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

公开(公告)号：US20100124763A1

公开(公告)日：2010-05-20

申请号：US12589968

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3577 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.

摘要翻译： 本发明提供了用于监测，检测和/或表征可能存在于样品中的生物颗粒的方法和系统。 该方法可以在密封容器中通过利用第一步骤时间依赖性光谱技术来实现，以获得包含样品的生长组合物的至少两个测量值，并将所述测量结果与生物学颗粒的检测和/或表征相关联，生物颗粒可以是 存在于样品中。 该方法还提供了在密封容器中分离，表征和/或鉴定微生物的后续步骤。