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    Methods for Inactiviation And Extraction of Acid-Fast Bacteria for Characterization And/Or Identification Using Mass Spectrometry
    1.
    发明申请
    Methods for Inactiviation And Extraction of Acid-Fast Bacteria for Characterization And/Or Identification Using Mass Spectrometry 有权
    使用质谱法鉴定和/或鉴定酸快速细菌的灭活和提取方法

    公开(公告)号：US20130309714A1

    公开(公告)日：2013-11-21

    申请号：US13828119

    申请日：2013-03-14

    申请人： Jones Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

    发明人： Jones Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

    IPC分类号： G01N1/40

    CPC分类号： G01N1/4077 ,  C12Q1/24 ,  G01N33/5695 ,  G01N33/6851 ,  G01N2333/35 ,  G01N2333/355 ,  G01N2560/00

    摘要： The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g., mycobacteria or nocardia), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.

    摘要翻译： 本发明涉及一种灭活和/或提取酸性抗菌（例如分枝杆菌或诺卡氏菌）的方法，该方法包括以下顺序步骤：（a）获得已知含有或可含有酸的测试样品 将试样悬浮在含有乙醇和珠粒的容器中; （b）珠子打浆和/或涡旋容器以分解块和/或破坏容器中的耐酸细菌细胞; 和（c）随后在室温下将悬浮液温育至少约3分钟以灭活测试样品中所含的任何耐酸细菌。 根据本发明，随后通过离心将测试样品沉淀，用甲酸重悬浮，加入乙腈，并进行质谱鉴定和/或鉴定耐酸细菌。

    Methods For Inactiviation And/or Extraction of A Fungus Test Sample For Characterization And/or Identification Using Mass Spectrometry
    5.
    发明申请
    Methods For Inactiviation And/or Extraction of A Fungus Test Sample For Characterization And/or Identification Using Mass Spectrometry 审中-公开
    用于表征和/或使用质谱鉴定的真菌试验样品的灭活和/或提取的方法

    公开(公告)号：US20130309716A1

    公开(公告)日：2013-11-21

    申请号：US13835620

    申请日：2013-03-15

    申请人： Valérie Monnin ,  Victoria Girard ,  Marcel Erhard ,  Maud Arsac

    发明人： Valérie Monnin ,  Victoria Girard ,  Marcel Erhard ,  Maud Arsac

    IPC分类号： G01N1/30

    CPC分类号： G01N1/30 ,  G01N33/6851 ,  G01N2333/37

    摘要： The present invention is directed to a method for inactivation and/or extraction of fungus samples (e.g., mold or yeast samples), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain fungus and suspending the test sample in a container containing water and/or suspension medium; (b) adding ethanol to the suspension; (c) centrifuging the container to pellet the fungus and removing and discarding the supernatant; (d) resuspending the fungus in formic acid; (e) adding acetonitrile to the sample; (f) centrifuging the sample; and (g) recovering the supernatant. In accordance with the present invention, the recovered supernatant can be subjected to mass spectrometry analysis for characterization and/or identification of the unknown fungus.

    摘要翻译： 本发明涉及一种用于灭活和/或提取真菌样品（例如霉菌或酵母样品）的方法，所述方法包括以下顺序步骤：（a）获得已知含有或可能含有真菌的试样， 将测试样品悬浮在含有水和/或悬浮介质的容器中; （b）向悬浮液中加入乙醇; （c）将容器离心以沉淀真菌并除去和弃去上清液; （d）将真菌重悬于甲酸中; （e）向样品中加入乙腈; （f）离心样品; 和（g）回收上清液。 根据本发明，可以对回收的上清液进行质谱分析，以鉴定和/或识别未知真菌。