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21 条记录 (耗时 0.034 秒)

    Methods for Inactiviation And Extraction of Acid-Fast Bacteria for Characterization And/Or Identification Using Mass Spectrometry
    1.
    发明申请
    Methods for Inactiviation And Extraction of Acid-Fast Bacteria for Characterization And/Or Identification Using Mass Spectrometry 有权
    使用质谱法鉴定和/或鉴定酸快速细菌的灭活和提取方法

    公开(公告)号：US20130309714A1

    公开(公告)日：2013-11-21

    申请号：US13828119

    申请日：2013-03-14

    申请人： Jones Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

    发明人： Jones Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

    IPC分类号： G01N1/40

    CPC分类号： G01N1/4077 ,  C12Q1/24 ,  G01N33/5695 ,  G01N33/6851 ,  G01N2333/35 ,  G01N2333/355 ,  G01N2560/00

    摘要： The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g., mycobacteria or nocardia), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.

    摘要翻译： 本发明涉及一种灭活和/或提取酸性抗菌（例如分枝杆菌或诺卡氏菌）的方法，该方法包括以下顺序步骤：（a）获得已知含有或可含有酸的测试样品 将试样悬浮在含有乙醇和珠粒的容器中; （b）珠子打浆和/或涡旋容器以分解块和/或破坏容器中的耐酸细菌细胞; 和（c）随后在室温下将悬浮液温育至少约3分钟以灭活测试样品中所含的任何耐酸细菌。 根据本发明，随后通过离心将测试样品沉淀，用甲酸重悬浮，加入乙腈，并进行质谱鉴定和/或鉴定耐酸细菌。

    Methods for the characterization of microorganisms on solid or semi-solid media
    3.
    发明授权
    Methods for the characterization of microorganisms on solid or semi-solid media 有权
    在固体或半固体培养基上表征微生物的方法

    公开(公告)号：US08795983B2

    公开(公告)日：2014-08-05

    申请号：US13959802

    申请日：2013-08-06

    申请人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

    发明人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

    IPC分类号： C12Q1/04

    CPC分类号： C12Q1/04

    摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

    摘要翻译： 本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。 所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

    Methods for Separation, Characterization and/or Identification of Microorganisms using Spectroscopy
    5.
    发明申请
    Methods for Separation, Characterization and/or Identification of Microorganisms using Spectroscopy 有权
    使用光谱分离，表征和/或鉴定微生物的方法

    公开(公告)号：US20140186879A1

    公开(公告)日：2014-07-03

    申请号：US14146367

    申请日：2014-01-02

    申请人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

    发明人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

    IPC分类号： C12Q1/04

    CPC分类号： C12Q1/04 ,  G01N33/6848 ,  H01J49/00

    摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

    摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

    Methods for separation, characterization and/or identification of microorganisms using spectroscopy
    6.
    发明授权
    Methods for separation, characterization and/or identification of microorganisms using spectroscopy 有权
    使用光谱法分离，表征和/或鉴定微生物的方法

    公开(公告)号：US08647835B2

    公开(公告)日：2014-02-11

    申请号：US13117976

    申请日：2011-05-27

    申请人： John Walsh ,  Jones Hyman

    发明人： John Walsh ,  Jones Hyman

    IPC分类号： C12Q1/04

    CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3563 ,  G01N21/3577 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/6486 ,  G01N21/65 ,  G01N2015/1402

    摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The method may also be useful for the physical separation and/or enrichment of two or more different or individual microorganism species contained in a mixed test sample. The invention further provides for spectroscopic interrogation of the separated microorganism sample(s) to produce measurements of the microorganism and characterizing and/or identifying the microorganism(s) in the sample using said spectroscopic measurements.

    摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 该方法还可用于混合测试样品中包含的两种或多种不同或单独的微生物物质的物理分离和/或富集。 本发明进一步提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

    Method for separation, characterization and/or identification of microorganisms using raman spectroscopy
    7.
    发明申请
    Method for separation, characterization and/or identification of microorganisms using raman spectroscopy 审中-公开
    使用拉曼光谱法分离，表征和/或鉴定微生物的方法

    公开(公告)号：US20100136609A1

    公开(公告)日：2010-06-03

    申请号：US12589976

    申请日：2009-10-30

    申请人： Bradford Clay ,  Jones Hyman ,  John Walsh ,  Thurman Thorpe ,  Christopher Ronsick

    发明人： Bradford Clay ,  Jones Hyman ,  John Walsh ,  Thurman Thorpe ,  Christopher Ronsick

    IPC分类号： C12Q1/04 ,  C12Q1/02

    CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

    摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for Raman spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said Raman spectroscopic measurements.

    摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供分离的微生物样品的拉曼光谱询问以产生微生物的测量，并使用所述拉曼光谱测量来表征和/或鉴定样品中的微生物。