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7 条记录 (耗时 0.016 秒)

    Colormetric method for quantification of magnesium using .alpha.-glycerophosphate oxidase
    1.
    发明授权
    Colormetric method for quantification of magnesium using .alpha.-glycerophosphate oxidase 失效
    使用α-甘油磷酸酯氧化酶定量镁的色谱法

    公开(公告)号：US4818691A

    公开(公告)日：1989-04-04

    申请号：US882072

    申请日：1986-07-03

    申请人： Joseph D. Artiss ,  Bennie Zak ,  Michael C. Wimmer

    发明人： Joseph D. Artiss ,  Bennie Zak ,  Michael C. Wimmer

    IPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q1/48 ,  G01N33/84

    CPC分类号： C12Q1/48 ,  C12Q1/26 ,  G01N33/84 ,  G01N2800/04

    摘要： A method and composition for determining magnesium levels in a liquid. The composition contains a first enzyme reagent part having glycerol-kinase, adenosine 5'-triphosphate, 4-aminoantipyrene, .alpha.-glycerophosphate oxidase, peroxidase, glycerol kinase, water and a buffer capable of maintaining the solution at a pH between about 5.5 and about 9.2. The composition also contains a second trigger reagent part which has sodium 2-hydroxy-3,5-dichlorobenzene sulfonate, glycerol, water and a buffer capable of maintaining the solution pH between about 5.5 and about 9.2. In the method of the present invention, a liquid to be analyzed is contacted sequentially with the first enzyme reagent part and followed by the second trigger reagent and is measured to quantitatively detect the presence of a detectable species produced as a result of the reactions triggered by the mixture of the enzyme reagent part and the trigger reagent part.

    摘要翻译： 一种用于测定液体中镁含量的方法和组合物。 该组合物含有具有甘油 - 激酶，腺苷5'-三磷酸，4-氨基芘，α-甘油磷酸氧化酶，过氧化物酶，甘油激酶，水和能够将溶液保持在约5.5和约pH之间的缓冲液的缓冲液的第一酶试剂部分 9.2。 该组合物还含有第二触发试剂部分，其具有2-羟基-3,5-二氯苯磺酸钠，甘油，水和能够将溶液pH保持在约5.5至约9.2之间的缓冲剂。 在本发明的方法中，待分析的液体依次与第一酶试剂部分接触，然后与第二触发试剂接触，并测量其定量检测由于由 酶试剂部分和触发试剂部分的混合物。

    Composition for reducing turbidity in samples of biological fluids
    3.
    发明授权
    Composition for reducing turbidity in samples of biological fluids 失效
    用于降低生物液体样品中浊度的组合物

    公开(公告)号：US5310679A

    公开(公告)日：1994-05-10

    申请号：US936267

    申请日：1986-12-01

    申请人： Joseph D. Artiss ,  Bennie Zak

    发明人： Joseph D. Artiss ,  Bennie Zak

    IPC分类号： C12Q1/44 ,  G01N33/48 ,  G01N33/50 ,  G01N31/00

    CPC分类号： G01N33/48 ,  C12Q1/44 ,  G01N33/50 ,  G01N2333/805 ,  G01N2400/18 ,  Y10T436/10 ,  Y10T436/104165 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/2525

    摘要： A composition which reduces the turbidity of samples of artificial or naturally occurring biological fluids comprising:a buffering agent;a nonionic surfactant;a lipolytic enzyme; and water.The composition provides a catalyst for and agents which hydrolyze triglycerides present in the sample to glycerol and free fatty acids. Also present in the composition is a fatty acid scavenging agent or agents to render the catalyzed fatty acids water-soluble. The fatty acid scavenging agent may be cyclodextrin, albumin or mixtures thereof.

    摘要翻译： 一种降低人造或天然存在的生物流体样品的浊度的组合物，包括：缓冲剂; 非离子表面活性剂; 一种脂解酶; 和水。 该组合物提供催化剂和将样品中存在的甘油三酯水解成甘油和游离脂肪酸的试剂。 还存在于组合物中的是脂肪酸清除剂或使催化的脂肪酸溶于水的试剂。 脂肪酸清除剂可以是环糊精，白蛋白或其混合物。

    Method and compositions for the determination of serum calcium using aersenazo III
    5.
    发明授权
    Method and compositions for the determination of serum calcium using aersenazo III 失效
    用aersenazo III测定血清钙的方法和组成

    公开(公告)号：US5215922A

    公开(公告)日：1993-06-01

    申请号：US831190

    申请日：1992-02-05

    申请人： Joseph D. Artiss ,  Bradley R. Morgan ,  Bennie Zak

    发明人： Joseph D. Artiss ,  Bradley R. Morgan ,  Bennie Zak

    IPC分类号： C12Q1/44 ,  G01N31/22 ,  G01N33/84

    CPC分类号： G01N31/22 ,  C12Q1/44 ,  G01N33/84 ,  G01N2400/18 ,  Y10T436/106664 ,  Y10T436/107497

    摘要： A colorimetric, particularly spectrophotometric, method and compositions for determining calcium levels in blood serum in the presence of magnesium and other serum ions is described. The method uses an aqueous composition with a pH between about 7.5 and 10 with arsenazo III to complex calcium and a hydroxyquinoline selected from the group consisting of 8-hydroxyquinoline and 8-hydroxyquinoline-5-sulfonate to complex magnesium. Lipase and a cyclodextrin can be used as reagents to clear triglycerides in the serum which can provide turbidity and false results in some instances. The method is particularly useful for human serum.

    摘要翻译： 描述了在镁和其它血清离子存在下测定血清中钙水平的比色，特别是分光光度法和组合物。 该方法使用具有约7.5至10的pH的含水组合物与砷砷化合物III配合，并将选自8-羟基喹啉和8-羟基喹啉-5-磺酸盐的羟基喹啉复合成镁。 脂肪酶和环糊精可用作清除血清中的甘油三酯的试剂，可在一些情况下提供浊度和假结果。 该方法对人血清特别有用。

    Novel amino acid substituted 4-aminophenazones for measuring enzyme activity
    6.
    发明授权
    Novel amino acid substituted 4-aminophenazones for measuring enzyme activity 失效
    用于测定酶活性的新型氨基酸取代的4-氨基苯扎嗪酮

    公开(公告)号：US5081259A

    公开(公告)日：1992-01-14

    申请号：US586995

    申请日：1990-09-24

    申请人： Joseph D. Artiss ,  Jill M. Bensie ,  Bennie Zak ,  Daniel S. Raden

    发明人： Joseph D. Artiss ,  Jill M. Bensie ,  Bennie Zak ,  Daniel S. Raden

    IPC分类号： C07K5/02 ,  C07K5/06 ,  C07K5/072

    CPC分类号： C07K5/06026 ,  C07K5/0215 ,  C07K5/06104

    摘要： Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4-aminophenazone reacts with the enzyme to produce 4-aminophenazone which is an amino dye intermediate. The amino dye intermediate is coupled with a second dye intermediate to form a chromogen. The 4-aminophenazone is particularly reacted with a phenolic naphtholic or aniline compound, particularly the phenolic compound 2-hydroxy-3,5-dichlorobenzenesulfonate, to form a red chromogen in the presence of an oxidizing agent, such as bilrubin oxidase.

    摘要翻译： 描述了用于检测酶，特别是L-γ-谷氨酰转移酶的氨基酸4-氨基吩噻嗪的新型底物。 氨基酸γ-谷氨酰-4-氨基苯甲酸与酶反应，生成作为氨基染料中间体的4-氨基苯甲酮。 氨基染料中间体与第二染料中间体偶联以形成色原体。 4-氨基苯甲酮与苯酚萘酚或苯胺化合物，特别是2-羟基-3,5-二氯苯磺酸酚化合物特别反应，在氧化剂如胆红素氧化酶存在下形成红色色原。