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31 条记录 (耗时 0.026 秒)

    Low-cost production of peptides
    1.
    发明授权
    Low-cost production of peptides 有权
    低成本生产肽

    公开(公告)号：US07595173B2

    公开(公告)日：2009-09-29

    申请号：US10971444

    申请日：2004-10-22

    申请人： Joseph F. Krebs ,  Paul S. Zorner ,  Ian A. Tomlinson

    发明人： Joseph F. Krebs ,  Paul S. Zorner ,  Ian A. Tomlinson

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C07K5/06

    CPC分类号： C07K14/4723 ,  C07K2319/35 ,  C07K2319/50

    摘要： The subject invention relates to a low cost method of producing peptides, including antimicrobial peptides (AMPs), by using microbes. The subject methods enable greatly improved yields of the peptide/AMP as compared to those heretofore known in the art. The subject methods also surprisingly enable the use of Pseudomonas fluorescens to produce AMPs and other peptides. There are several components of the subject invention, which can be used alone or in combination. The subject invention provides for the production of peptides/AMPs in concatemeric precursors. The subject invention also provides novel methods of assembling monomers into multimers, and of cleaving the multimers to yield active monomers. The subject invention also relates to the use of these multimers fused to carrier peptides to produce fusion proteins. Preferably, both the multimers and the fusion proteins (multimers with the carrier polypeptides) lack charge balancing. It has been surprisingly determined that it is not necessary to offset the positive charges of multiple copies of AMPs in multimeric constructs. Thus, the subject invention enables the use of a wider range of multimers and carrier peptides.

    摘要翻译： 本发明涉及通过使用微生物生产肽（包括抗微生物肽（AMP））的低成本方法。 与本领域已知的方法相比，本发明方法能够大大提高肽/ AMP的产率。 本发明的方法也令人惊奇地使得能够使用荧光假单胞菌来产生AMP和其他肽。 本发明的几个组分可单独使用或组合使用。 本发明提供了在并联前体中生产肽/ AMP。 本发明还提供了将单体组装成多聚体的新方法，以及裂解多聚体以产生活性单体。 本发明还涉及这些与载体肽融合的多聚体产生融合蛋白的用途。 优选地，多聚体和融合蛋白（携带多肽的多聚体）都缺乏电荷平衡。 已经令人惊讶地确定，不需要在多聚体构建体中抵消多个拷贝的AMP的正电荷。 因此，本发明使得能够使用更宽范围的多聚体和载体肽。

    Synergistic triazole compounds
    9.
    发明授权
    Synergistic triazole compounds 失效
    协同三唑化合物

    公开(公告)号：US4988818A

    公开(公告)日：1991-01-29

    申请号：US418225

    申请日：1989-10-06

    申请人： Manfred Lauer ,  Matthias Zipplies ,  Hubert Sauter ,  Barbara A. Moore ,  Dale R. Carlson ,  Paul S. Zorner ,  Karl-Otto Westphalen ,  Bruno Wuerzer

    发明人： Manfred Lauer ,  Matthias Zipplies ,  Hubert Sauter ,  Barbara A. Moore ,  Dale R. Carlson ,  Paul S. Zorner ,  Karl-Otto Westphalen ,  Bruno Wuerzer

    IPC分类号： A01N43/653 ,  A01N43/88 ,  C07D249/08 ,  C07D521/00

    CPC分类号： C07D231/12 ,  A01N43/653 ,  A01N43/88 ,  C07D233/56 ,  C07D249/08

    摘要： Triazole compounds I ##STR1## where A is C.sub.7 -C.sub.20 -alkyl or unsubstituted or substituted phenyl, naphthyl or pyridyl, and R is hydrogen or unsubstituted or substituted C.sub.1 -C.sub.6 -alkyl, C.sub.3 -C.sub.6 -alkenyl or C.sub.3 -C.sub.6 -alkynyl or is --COR.sup.x or --SO.sub.2 R.sup.x, where R.sup.x is hydrogen or unsubstituted or substituted C.sub.1 -C.sub.8 -alkyl or phenyl, and their environmentally compatible salts, and herbicides containing one or more triazole compounds I, IA or IB ##STR2## where R.sup.1 is hydrogen, cyclopropyl or unsubstituted or substituted C.sub.1 -C.sub.6 -alkyl, C.sub.2 -C.sub.6 -alkenyl or C.sub.2 -C.sub.6 -alkynyl or is C.sub.1 -C.sub.4 -alkoxy, C.sub.2 -C.sub.4 -alkenyloxy or C.sub.2 -C.sub.4 -alkynyloxy, and R.sup.2 and R.sup.3 are each hydrogen, adamantyl or C.sub.1 -C.sub.3 -alkyl or, together with the carbon atom to which they are bonded, form unsubstituted or substituted C.sub.3 -C.sub.7 -cycloalkyl, C.sub.6 -C.sub.10 -bicycloalkyl, C.sub.6 -C.sub.10 -bicycloalkenyl, or their environmentally compatible salts and a benzothiadiazone.

    摘要翻译： 三唑化合物其中A为C 7 -C 20烷基或未取代或取代的苯基，萘基或吡啶基，R为氢或未取代或取代的C 1 -C 6 - 烷基，C 3 -C 6 - 烯基或C 3 -C 6 - 炔基 或者是-COR x或-SO 2 R x，其中R x是氢或未取代或取代的C 1 -C 8 - 烷基或苯基，以及它们的环境相容的盐和含有一种或多种三唑化合物I，IA或IB的除草剂。 其中R1是氢，环丙基或未取代的或取代的C1-C6-烷基，C2-C6-烯基或C2-C6-炔基或是C1-C4-烷氧基，C2-C4-烯氧基或C2-C4-炔氧基，R2 C 3 -C 7 - 环烷基，C 6 -C 10 - 双环烯基或它们的连接基团的碳原子一起形成未取代或取代的C 3 -C 7 - 环烷基，C 6 -C 10 - 双环烷基，C 6 -C 10 - 环境相容的盐和苯并噻二唑。