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    Diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS)
    3.
    发明授权
    Diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS) 有权
    导致严重急性呼吸综合征（SARS）的人类病毒的诊断测定

    公开(公告)号：US07267942B2

    公开(公告)日：2007-09-11

    申请号：US10808187

    申请日：2004-03-24

    申请人： Joseph S. M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls

    发明人： Joseph S. M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls

    IPC分类号： C12Q1/70 ,  C12N15/50

    CPC分类号： C12Q1/701 ,  C07H21/04 ,  C12Q1/6844

    摘要： The present invention relates to a diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). In particular, the invention relates to a real-time quantitative PCR assay for the detection of hSARS virus using reverse transcription and polymerase chain reaction. Specifically, the quantitative assay is a TaqMan® assay using the primers and probes constructed based on the genome of the hSARS virus. The invention further relates to a diagnostic kit that comprises nucleic acid molecules for the detection of the hSARS virus.

    摘要翻译： 本发明涉及在人类中引起严重急性呼吸综合征（SARS）的病毒的诊断测定法（“hSARS病毒”）。 特别地，本发明涉及使用逆转录和聚合酶链反应检测hSARS病毒的实时定量PCR测定。 具体地，定量测定是使用基于hSARS病毒基因组构建的引物和探针的TaqMan测定法。 本发明还涉及包含用于检测hSARS病毒的核酸分子的诊断试剂盒。

    High-throughput diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS)
    4.
    发明授权
    High-throughput diagnostic assay for the human virus causing severe acute respiratory syndrome (SARS) 有权
    导致严重急性呼吸综合征（SARS）的人类病毒的高通量诊断测定

    公开(公告)号：US07547512B2

    公开(公告)日：2009-06-16

    申请号：US10807807

    申请日：2004-03-24

    申请人： Joseph S. M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

    发明人： Joseph S. M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

    IPC分类号： C12Q1/68 ,  C12Q1/00

    CPC分类号： C12Q1/701 ,  C12Q1/6844 ,  Y02A50/451

    摘要： The present invention relates to a high-throughput diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). In particular, the invention relates to a high-throughput reverse transcription-PCR diagnostic test for SARS associated coronavirus (SARS-CoV). The present assay is a rapid, reliable assay which can be used for diagnosis and monitoring the spread of SARS and is based on the nucleotide sequences of the N (nucleocapsid)-gene of the hSARS virus. The present method eliminates false negative results and provides increased sensitivity for the assay. The invention also discloses the S (spike)-gene of the hSARS virus. The invention further relates to the deduced amino acid sequences of the N-gene and S-gene products of the hSARS virus and to the use of the N-gene and S-gene products in diagnostic methods. The invention further encompasses diagnostic assays and kits comprising antibodies generated against the N-gene or S-gene product.

    摘要翻译： 本发明涉及用于引起人类严重急性呼吸综合征（SARS）的病毒（“hSARS病毒”）的高通量诊断测定。 特别地，本发明涉及SARS相关冠状病毒（SARS-CoV）的高通量逆转录PCR诊断试验。 本试验是一种快速，可靠的测定法，可用于诊断和监测SARS的扩散，并且基于hSARS病毒的N（核衣壳）基因的核苷酸序列。 本方法消除假阴性结果并提供增加的检测灵敏度。 本发明还公开了hSARS病毒的S（尖峰）基因。 本发明还涉及hSARS病毒的N-基因和S-基因产物的推断氨基酸序列以及在诊断方法中使用N-基因和S-基因产物。 本发明还包括诊断测定和试剂盒，其包含针对N-基因或S-基因产物产生的抗体。

    High-Throughput Diagnostic Assay For the Human Virus Causing Severe Acute Respiratory Syndrome (SARS)
    5.
    发明申请
    High-Throughput Diagnostic Assay For the Human Virus Causing Severe Acute Respiratory Syndrome (SARS) 审中-公开
    用于严重急性呼吸综合征（SARS）的人类病毒的高通量诊断测定

    公开(公告)号：US20100285457A1

    公开(公告)日：2010-11-11

    申请号：US12483301

    申请日：2009-06-12

    申请人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

    发明人： Joseph S.M. Peiris ,  Kwok Yung Yuen ,  Lit Man Poon ,  Yi Guan ,  Kwok Hung Chan ,  John M. Nicholls ,  Frederick C. Leung

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/701 ,  C12Q1/6844 ,  Y02A50/451

    摘要： The present invention relates to a high-throughput diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). In particular, the invention relates to a high-throughput reverse transcription-PCR diagnostic test for SARS associated coronavirus (SARS-CoV). The present assay is a rapid, reliable assay which can be used for diagnosis and monitoring the spread of SARS and is based on the nucleotide sequences of the N (nucleocapsid)-gene of the hSARS virus. The present method eliminates false negative results and provides increased sensitivity for the assay. The invention also discloses the S (spike)-gene of the hSARS virus. The invention further relates to the deduced amino acid sequences of the N-gene and S-gene products of the hSARS virus and to the use of the N-gene and S-gene products in diagnostic methods. The invention further encompasses diagnostic assays and kits comprising antibodies generated against the N-gene or S-gene product.

    摘要翻译： 本发明涉及用于引起人类严重急性呼吸综合征（SARS）的病毒（“hSARS病毒”）的高通量诊断测定。 特别地，本发明涉及SARS相关冠状病毒（SARS-CoV）的高通量逆转录PCR诊断试验。 本试验是一种快速，可靠的测定法，可用于诊断和监测SARS的扩散，并且基于hSARS病毒的N（核衣壳）基因的核苷酸序列。 本方法消除假阴性结果并提供增加的检测灵敏度。 本发明还公开了hSARS病毒的S（尖峰） - 基因。 本发明还涉及hSARS病毒的N-基因和S-基因产物的推断氨基酸序列以及在诊断方法中使用N-基因和S-基因产物。 本发明还包括诊断测定和试剂盒，其包含针对N-基因或S-基因产物产生的抗体。

    Human virus causing respiratory tract infection and uses thereof
    10.
    发明授权
    Human virus causing respiratory tract infection and uses thereof 有权
    引起呼吸道感染的人类病毒及其用途

    公开(公告)号：US07553944B2

    公开(公告)日：2009-06-30

    申请号：US10895064

    申请日：2004-07-21

    申请人： Kwok Yung Yuen ,  Chiu Yat Patrick Woo ,  Kar Pui Susanna Lau ,  Kwok Hung Chan ,  Lit Man Poon ,  Joseph Sriyal Malik Peiris ,  Yi Guan

    发明人： Kwok Yung Yuen ,  Chiu Yat Patrick Woo ,  Kar Pui Susanna Lau ,  Kwok Hung Chan ,  Lit Man Poon ,  Joseph Sriyal Malik Peiris ,  Yi Guan

    IPC分类号： C07H21/02 ,  C12N15/00 ,  A61K39/00 ,  A61K39/12

    CPC分类号： G01N33/56983 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/20021 ,  C12N2770/20022

    摘要： The present invention provides the complete genomic sequence of a novel human coronavirus, coined as coronavirus-HKU1 (“CoV-HKU1”), isolated in Hong Kong from a patient who had a recent history of visit to Schenzhen, China. The virus belongs to the order Nidovirales of the family Coronaviridae, being a single-stranded RNA virus of positive polarity. The invention also provides the deduced amino acid sequences of the complete genome of the CoV-HKU1. The nucleotide sequences and deduced amino acid sequences of the CoV-HKU1 are useful in preventing, diagnosing and/or treating the infection by CoV-HKU1. Furthermore, the invention provides immunogenic and vaccine preparations using recombinant and chimeric forms as well as subunits of the CoV-HKU1 based on the nucleotide sequences and deduced amino acid sequences of the CoV-HKU1.

    摘要翻译： 本发明提供了一种新型人冠状病毒的完整基因组序列，其创造为冠状病毒HKU1（“CoV-HKU1”），在香港从最近访问中国的深圳的病人中分离出来。 该病毒属于冠状病毒科家族的Nidovirales，是正极性的单链RNA病毒。 本发明还提供了CoV-HKU1的完整基因组的推导的氨基酸序列。 CoV-HKU1的核苷酸序列和推导的氨基酸序列可用于预防，诊断和/或治疗CoV-HKU1感染。 此外，本发明基于CoV-HKU1的核苷酸序列和推定的氨基酸序列，提供使用重组和嵌合形式以及CoV-HKU1的亚基的免疫原性和疫苗制剂。