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17 条记录 (耗时 0.041 秒)

    Circular nucleic acid vectors, and methods for making and using the same
    5.
    发明授权
    Circular nucleic acid vectors, and methods for making and using the same 有权
    圆形核酸载体及其制备和使用方法

    公开(公告)号：US08828726B2

    公开(公告)日：2014-09-09

    申请号：US11398490

    申请日：2006-04-04

    申请人： Mark A. Kay ,  Zhi-Ying Chen

    发明人： Mark A. Kay ,  Zhi-Ying Chen

    IPC分类号： A61K48/00 ,  C12N15/85

    CPC分类号： C12N15/64 ,  A61K48/005 ,  C12N15/70 ,  C12N15/85 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2830/002 ,  C12N2830/55

    摘要： Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same.

    摘要翻译： 提供了提供持续高水平蛋白质表达的圆形核酸载体。 本发明的圆形载体的特征在于没有表达沉默细菌序列，其中在许多实施方案中，除了表达盒之外，主题载体还包括单向位点特异性重组产物杂合序列。 还提供了使用本发明载体将核酸例如表达盒引入靶细胞的方法，以及用于实施这些方法的制剂。 主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。 还提供了用于生产本发明方法中使用的载体的高效且易于扩展的方法，以及用于实施该载体的试剂和试剂盒/系统。

    Minicircle DNA vector preparations and methods of making and using the same
    6.
    发明授权
    Minicircle DNA vector preparations and methods of making and using the same 有权
    小环DNA载体制剂及其制备和使用方法

    公开(公告)号：US08236548B2

    公开(公告)日：2012-08-07

    申请号：US12497396

    申请日：2009-07-02

    申请人： Zhi-Ying Chen ,  Mark A. Kay

    发明人： Zhi-Ying Chen ,  Mark A. Kay

    IPC分类号： C12N1/21

    CPC分类号： C12N15/70 ,  A61K48/005 ,  A61K48/0066 ,  C07K14/195 ,  C12N15/85 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2830/002 ,  C12N2830/55

    摘要： The present invention provides minicircle nucleic acid vector formulations for use in administering to a subject, wherein the minicircle nucleic acid vectors include a polynucleotide of interest, a product hybrid sequence of a unidirectional site-specific recombinase, and are devoid of plasmid backbone bacterial DNA sequences. Also provided are methods of producing the subject formulations as well as methods for administering the minicircle nucleic acid vector formulations to a subject. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications.

    摘要翻译： 本发明提供了用于向受试者施用的小环核酸载体制剂，其中小环核酸载体包括目的多核苷酸，单向位点特异性重组酶的产物杂交序列，并且没有质粒主链细菌DNA序列 。 还提供了制备本发明制剂的方法以及向受试者施用小环核酸载体制剂的方法。 主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。

    Circular nucleic acid vectors, and methods for making and using the same
    7.
    发明授权
    Circular nucleic acid vectors, and methods for making and using the same 有权
    圆形核酸载体及其制备和使用方法

    公开(公告)号：US08124407B2

    公开(公告)日：2012-02-28

    申请号：US13006356

    申请日：2011-01-13

    申请人： Mark A. Kay ,  Zhi-Ying Chen

    发明人： Mark A. Kay ,  Zhi-Ying Chen

    IPC分类号： C12N15/00

    CPC分类号： C12N15/64 ,  A61K48/005 ,  C12N15/70 ,  C12N15/85 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2830/002 ,  C12N2830/55

    摘要： Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same.

    摘要翻译： 提供了提供持续高水平蛋白质表达的圆形核酸载体。 本发明的圆形载体的特征在于没有表达沉默细菌序列，其中在许多实施方案中，除了表达盒之外，主题载体还包括单向位点特异性重组产物杂合序列。 还提供了使用本发明载体将核酸例如表达盒引入靶细胞的方法，以及用于实施这些方法的制剂。 主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。 还提供了用于生产本发明方法中使用的载体的高效且易于扩展的方法，以及用于实施本发明方法的试剂和试剂盒/系统。

    Circular Nucleic Acid Vectors, and Methods for Making and Using the Same
    8.
    发明申请
    Circular Nucleic Acid Vectors, and Methods for Making and Using the Same 有权
    环状核酸载体及其制备和使用方法

    公开(公告)号：US20110126306A1

    公开(公告)日：2011-05-26

    申请号：US13006356

    申请日：2011-01-13

    申请人： Mark A. Kay ,  Zhi-Ying Chen

    发明人： Mark A. Kay ,  Zhi-Ying Chen

    IPC分类号： C12N15/00 ,  C12N15/63

    CPC分类号： C12N15/64 ,  A61K48/005 ,  C12N15/70 ,  C12N15/85 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2830/002 ,  C12N2830/55

    摘要： Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are to characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same.

    摘要翻译： 提供了提供持续高水平蛋白质表达的圆形核酸载体。 本发明的圆形载体的特征在于没有表达沉默细菌序列，其中在许多实施方案中，除了表达盒之外，主题载体还包括单向位点特异性重组产物杂合序列。 还提供了使用本发明载体将核酸例如表达盒引入靶细胞的方法，以及用于实施这些方法的制剂。 主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。 还提供了用于生产本发明方法中使用的载体的高效且易于扩展的方法，以及用于实施本发明方法的试剂和试剂盒/系统。

    Minicircle DNA vector preparations and methods of making and using the same
    9.
    发明授权
    Minicircle DNA vector preparations and methods of making and using the same 有权
    小环DNA载体制剂及其制备和使用方法

    公开(公告)号：US08945885B2

    公开(公告)日：2015-02-03

    申请号：US13567908

    申请日：2012-08-06

    申请人： Zhi-Ying Chen ,  Mark A. Kay

    发明人： Zhi-Ying Chen ,  Mark A. Kay

    IPC分类号： C12N15/64 ,  C12N15/70 ,  A61K48/00 ,  C07K14/195 ,  C12N15/85

    CPC分类号： A61K48/0066 ,  A61K48/005 ,  C07K14/195 ,  C12N15/70 ,  C12N15/85 ,  C12N2800/108 ,  C12N2800/24 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2830/002 ,  C12N2830/55

    摘要： The present invention provides minicircle nucleic acid vector formulations for use in administering to a subject, wherein the minicircle nucleic acid vectors include a polynucleotide of interest, a product hybrid sequence of a unidirectional site-specific recombinase, and are devoid of plasmid backbone bacterial DNA sequences. Also provided are methods of producing the subject formulations as well as methods for administering the minicircle nucleic acid vector formulations to a subject. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications.

    摘要翻译： 本发明提供了用于向受试者施用的小环核酸载体制剂，其中小环核酸载体包括目的多核苷酸，单向位点特异性重组酶的产物杂交序列，并且没有质粒主链细菌DNA序列 。 还提供了制备本发明制剂的方法以及向受试者施用小环核酸载体制剂的方法。 主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。

    Circular nucleic acid vectors, and methods for making and using the same
    10.
    发明授权
    Circular nucleic acid vectors, and methods for making and using the same 有权
    圆形核酸载体及其制备和使用方法

    公开(公告)号：US07897380B2

    公开(公告)日：2011-03-01

    申请号：US10652729

    申请日：2003-08-28

    申请人： Mark A. Kay ,  Zhi-Ying Chen

    发明人： Mark A. Kay ,  Zhi-Ying Chen

    IPC分类号： C12N15/00

    CPC分类号： C12N15/64 ,  A61K48/005 ,  C12N15/70 ,  C12N15/85 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2830/002 ,  C12N2830/55

    摘要： Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same.

    摘要翻译： 提供了提供持续高水平蛋白质表达的圆形核酸载体。 本发明的圆形载体的特征在于没有表达沉默细菌序列，其中在许多实施方案中，除了表达盒之外，主题载体还包括单向位点特异性重组产物杂合序列。 还提供了使用本发明载体将核酸例如表达盒引入靶细胞的方法，以及用于实施这些方法的制剂。 主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。 还提供了用于生产本发明方法中使用的载体的高效且易于扩展的方法，以及用于实施本发明方法的试剂和试剂盒/系统。