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公开(公告)号：US4895800A
公开(公告)日：1990-01-23
申请号：US801713
申请日：1985-11-26
IPC分类号： C12N15/09 , A61K39/29 , C07H21/04 , C07K14/02 , C12N1/16 , C12N1/19 , C12N15/00 , C12N15/51 , C12N15/81 , C12P21/00 , C12R1/84
CPC分类号： C07K14/005 , C12N15/815 , C12N2730/10122
摘要： Novel DNA constructs comprising regulatory regions plus the structural coding region for hepatitis B surface antigen (HBsAg) are disclosed. The regulatory regions employed are responsive to methanol, non-catabolite repressing carbon sources and catabolite repressing carbon sources followed by carbon source starvation. The novel constructs are incorporated into a variety of linear and circular plasmids. Such plasmids are used to transform suitable hosts and ultimately used for the production and isolation of hepatitis B surface antigen in high yields.
摘要翻译： 公开了包含调节区加上乙型肝炎表面抗原结构编码区（HBsAg）的新型DNA构建体。 所采用的调节区域对甲醇，非分解代谢物抑制碳源和分解代谢物抑制碳源，随后碳源不足有反应。 将这些新型构建体并入多种线性和环状质粒。 这种质粒用于转化合适的宿主，最终以高产率用于生产和分离乙型肝炎表面抗原。
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公开(公告)号：US5258302A
公开(公告)日：1993-11-02
申请号：US560618
申请日：1990-07-30
申请人： Thomas S. Vedvick , Michael E. Engel , Mary S. Urcan , Richard G. Buckholz , Jennifer A. Kinney
发明人： Thomas S. Vedvick , Michael E. Engel , Mary S. Urcan , Richard G. Buckholz , Jennifer A. Kinney
IPC分类号： A61K38/00 , C07K14/395 , C07K14/81 , C12N15/15 , C12N15/81 , C07K7/10 , C12N1/19 , C12P21/02
CPC分类号： C12N15/815 , C07K14/395 , C07K14/8117 , A61K38/00 , C07K2319/02
摘要： Biologically active aprotinin (APR) molecules, naturally occurring, relatively short, single chain polypeptides, are prepared by growing methylotrophic yeast transformants containing in their genome at least one copy of a DNA sequence operably encoding APR, in operational association with a DNA sequence encoding the S. cerevisiae alpha mating factor pre-pro sequence (including a processing sequence selected from lys-arg-(glu-ala).sub.x, wherein x is an integer falling in the range of 0-3), both under the regulation of a promoter region of a gene of a methylotrophic yeast, under conditions allowing expression of said DNA sequences, and secretion of APR molecules into the culture medium.Also disclosed are novel DNA fragments and novel recombinant yeast strains which are useful in the practice of the present invention.
摘要翻译： 生物活性抑肽酶（APR）分子，天然存在的，相对短的单链多肽，是通过生长甲基营养酵母转化体来制备的，所述转基因在其基因组中含有至少一个可操作地编码APR的DNA序列拷贝，与编码 酿酒酵母α交配因子前序序列（包括选自lys-arg-（glu-ala）x的加工序列，其中x是0-3范围内的整数），两者均在启动子 区域的甲基营养酵母的基因，允许所述DNA序列表达的条件和APR分子分泌到培养基中。 还公开了可用于本发明实践的新型DNA片段和新型重组酵母菌株。
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3.发明授权Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same 失效巴斯德毕赤酵母酸性磷酸酶基因，基因区，信号序列和包含其的表达载体
公开(公告)号：US5268273A
公开(公告)日：1993-12-07
申请号：US627539
申请日：1990-12-14
申请人： Richard G. Buckholz
发明人： Richard G. Buckholz
CPC分类号： C12N15/815 , C12N9/16 , Y10S435/938
摘要： The present invention discloses the Pichia pastoris acid phospbatase gene, which includes the 5' regulatory region, signal sequence, structural gene, and 3' transcription termination sequence. Also disclosed are methods of using these fragments, which include but are not limited to the secretion of proteins from cells and the regulation of the transcription of DNA. DNA vectors containing the acid phospbatase gene or fragments thereof and hosts transformed with these vectors are also disclosed. Additionally, integrative vectors which direct integration at the Pichia pastoris PHO1 locus and a method of identifying these disruptants is disclosed.
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4.发明授权Production of epideramal growth factor in pichia pastoris yeast cells 失效在PICHIA PASTORIS YEAST细胞中生产疟原虫生长因子
公开(公告)号：US5102789A
公开(公告)日：1992-04-07
申请号：US323964
申请日：1989-03-15
CPC分类号： C12N9/0089 , C07K14/485 , C12N15/815 , C12N9/2462
摘要： Epidermal Growth Factor (EGF) is produced by recombinant DNA technology in Pichia pastoris yeast cells.
摘要翻译： 表皮生长因子（EGF）通过重组DNA技术在毕赤酵母酵母细胞中产生。
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