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    APTAMER- AND NUCLEIC ACID ENZYME-BASED SYSTEMS FOR SIMULTANEOUS DETECTION OF MULTIPLE ANALYTES
    7.
    发明申请
    APTAMER- AND NUCLEIC ACID ENZYME-BASED SYSTEMS FOR SIMULTANEOUS DETECTION OF MULTIPLE ANALYTES 有权
    用于同时检测多种分析物的APTAMER-和核酸酶基系统

    公开(公告)号：US20090197261A1

    公开(公告)日：2009-08-06

    申请号：US12109171

    申请日：2008-04-24

    申请人： Yi Lu ,  Juewen Liu

    发明人： Yi Lu ,  Juewen Liu

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： G01N33/58 ,  B82Y15/00 ,  G01N33/588 ,  G01N2458/10

    摘要： The present invention provides aptamer- and nucleic acid enzyme-based systems for simultaneously determining the presence and optionally the concentration of multiple analytes in a sample. Methods of utilizing the system and kits that include the sensor components are also provided. The system includes a first reactive polynucleotide that reacts to a first analyte; a second reactive polynucleotide that reacts to a second analyte; a third polynucleotide; a fourth polynucleotide; a first particle, coupled to the third polynucleotide; a second particle, coupled to the fourth polynucleotide; and at least one quencher, for quenching emissions of the first and second quantum dots, coupled to the first and second reactive polynucleotides. The first particle includes a quantum dot having a first emission wavelength. The second particle includes a second quantum dot having a second emission wavelength different from the first emission wavelength. The third polynucleotide and the fourth polynucleotide are different.

    摘要翻译： 本发明提供了基于适体和核酸酶的系统，用于同时测定样品中多种分析物的存在和任选的浓度。 还提供了使用包括传感器部件的系统和套件的方法。 该系统包括与第一分析物反应的第一反应性多核苷酸; 与第二分析物反应的第二反应性多核苷酸; 第三多核苷酸; 第四个多核苷酸; 与第三多核苷酸偶联的第一颗粒; 与第四多核苷酸偶联的第二颗粒; 和至少一个猝灭剂，用于猝灭与第一和第二反应性多核苷酸偶联的第一和第二量子点的发射。 第一粒子包括具有第一发射波长的量子点。 第二粒子包括具有与第一发射波长不同的第二发射波长的第二量子点。 第三多核苷酸和第四多核苷酸是不同的。

    Biosensors based on directed assembly of particles
    8.
    发明授权
    Biosensors based on directed assembly of particles 有权
    基于有序组装的生物传感器

    公开(公告)号：US07485419B2

    公开(公告)日：2009-02-03

    申请号：US10756825

    申请日：2004-01-13

    申请人： Yi Lu ,  Juewen Liu

    发明人： Yi Lu ,  Juewen Liu

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/113 ,  C12N15/115 ,  C12N2310/121 ,  C12N2310/16 ,  C12N2310/3519 ,  C12Q1/005

    摘要： A sensor system for detecting an effector or cofactor comprises (a) a nucleic acid enzyme; (b) a substrate for the nucleic acid enzyme, comprising a first polynucleotide; (c) a first set of particles comprising a second polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 3′ terminus; and (d) a second set of particles comprising a third polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 5′ terminus.

    摘要翻译： 用于检测效应子或辅因子的传感器系统包括（a）核酸酶; （b）核酸酶的底物，其包含第一多核苷酸; （c）第一组颗粒，其包含与底物至少部分互补的第二多核苷酸，其中所述多核苷酸在其3'末端附着于颗粒; 和（d）第二组颗粒，其包含与底物至少部分互补的第三个多核苷酸，其中多核苷酸在其5'末端附着于颗粒。

    Phosphorothioate DNAzyme complexes and use thereof
    9.
    发明授权
    Phosphorothioate DNAzyme complexes and use thereof 有权

    公开(公告)号：US11142544B2

    公开(公告)日：2021-10-12

    申请号：US15307499

    申请日：2015-04-28

    申请人： Juewen Liu ,  Po-Jung Jimmy Huang

    发明人： Juewen Liu ,  Po-Jung Jimmy Huang

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N15/11 ,  C12N15/10

    摘要： A novel substrate-bound DNAzyme complex is provided comprising a DNAzyme bound to a nucleic acid-based substrate. The DNAzyme comprises a pair of binding arms which hybridize to binding regions on the substrate, and a catalytic domain between the binding arms. The nucleic acid-based substrate comprises a phosphorothioate-modified ribonucleotide cleavage site between the binding regions of the substrate. The catalytic domain of the DNAzyme catalyzes heavy metal-dependent cleavage of the substrate cleavage site. The DNAzyme complex is useful in a method of heavy metal sensing. A novel cadmium-selective DNAzyme is also described for cadmium sensing.