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    Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus
    1.
    发明授权
    Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus 有权
    使用电渗析法调节盐浓度的装置，包括该盐浓度的实验室的方法以及使用该装置调节盐浓度的方法

    公开(公告)号：US07722751B2

    公开(公告)日：2010-05-25

    申请号：US11440872

    申请日：2006-05-25

    申请人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

    发明人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

    IPC分类号： B01D61/48

    CPC分类号： B01D61/422 ,  B01D61/46 ,  B01L3/5027 ,  G01N2001/4011

    摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.

    摘要翻译： 提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。 该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。 即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

    Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus
    2.
    发明申请
    Apparatus for regulating salt concentration using electrodialysis, lab-on-a-chip including the same, and method of regulating salt concentration using the apparatus 有权
    使用电渗析法调节盐浓度的装置，包括该盐浓度的实验室的方法以及使用该装置调节盐浓度的方法

    公开(公告)号：US20060266650A1

    公开(公告)日：2006-11-30

    申请号：US11440872

    申请日：2006-05-25

    申请人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

    发明人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

    IPC分类号： B01D61/42

    CPC分类号： B01D61/422 ,  B01D61/46 ,  B01L3/5027 ,  G01N2001/4011

    摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.

    摘要翻译： 提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。 该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。 即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

    Method of regulating pH of fluid using the microfluidic device
    3.
    发明授权
    Method of regulating pH of fluid using the microfluidic device 有权
    使用微流体装置调节流体pH值的方法

    公开(公告)号：US08764956B2

    公开(公告)日：2014-07-01

    申请号：US13348168

    申请日：2012-01-11

    申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chin-sung Park ,  Jung-im Han ,  Seung-yeon Yang ,  Shin-i Yoo

    发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chin-sung Park ,  Jung-im Han ,  Seung-yeon Yang ,  Shin-i Yoo

    IPC分类号： B01L3/00

    CPC分类号： B01L3/5027 ,  B01L2200/06

    摘要： A microfluidic device for electrochemically regulating the pH of a fluid includes: an ion-exchange material; an anode chamber having a surface defined by a surface of the ion-exchange material and an anode electrode disposed along an edge of the surface of the anode chamber; and a cathode chamber having a surface defined by an opposite surface of the ion-exchange material and a cathode electrode disposed along an edge of the surface of the cathode chamber.

    摘要翻译： 用于电化学调节流体pH的微流体装置包括：离子交换材料; 具有由所述离子交换材料的表面限定的表面的阳极室和沿着所述阳极室的表面的边缘设置的阳极电极; 以及阴极室，其具有由离子交换材料的相对表面限定的表面和沿着阴极室的表面的边缘设置的阴极电极。

    Microfluidic device for electrically regulating the pH of a fluid therein and method of regulating the pH of a fluid in a microfluidic device using the same
    6.
    发明申请
    Microfluidic device for electrically regulating the pH of a fluid therein and method of regulating the pH of a fluid in a microfluidic device using the same 有权
    用于电调节其中流体pH值的微流体装置及使用其调节微流体装置中的流体pH值的方法

    公开(公告)号：US20070068813A1

    公开(公告)日：2007-03-29

    申请号：US11472182

    申请日：2006-06-21

    申请人： Jung-im Han ,  Joon-ho Kim

    发明人： Jung-im Han ,  Joon-ho Kim

    IPC分类号： C07K1/26 ,  G01N27/00

    CPC分类号： G05D21/02

    摘要： Provided is a microfluidic device for electrically regulating the pH of a fluid comprising: a first chamber comprising a first electrode; a second chamber comprising a second electrode; a third chamber comprising a third electrode, a metal ion exchange membrane between the first and second chambers; and a hydrogen ion exchange membrane between the first or second chamber and the third chamber.

    摘要翻译： 提供一种用于电流调节流体pH的微流体装置，包括：第一室，包括第一电极; 第二室，包括第二电极; 第三室，包括第三电极，在第一和第二室之间的金属离子交换膜; 以及在第一或第二室与第三室之间的氢离子交换膜。

    Microfluidic device for electrically regulating the pH of a fluid therein and method of regulating the pH of a fluid in a microfluidic device using the same
    7.
    发明授权
    Microfluidic device for electrically regulating the pH of a fluid therein and method of regulating the pH of a fluid in a microfluidic device using the same 有权
    用于电调节其中流体pH值的微流体装置及使用其调节微流体装置中的流体pH值的方法

    公开(公告)号：US08293095B2

    公开(公告)日：2012-10-23

    申请号：US11472182

    申请日：2006-06-21

    申请人： Jung-im Han ,  Joon-ho Kim

    发明人： Jung-im Han ,  Joon-ho Kim

    IPC分类号： G01N27/44

    CPC分类号： G05D21/02

    摘要： Provided is a microfluidic device for electrically regulating the pH of a fluid comprising: a first chamber comprising a first electrode; a second chamber comprising a second electrode; a third chamber comprising a third electrode, a metal ion exchange membrane between the first and second chambers; and a hydrogen ion exchange membrane between the first or second chamber and the third chamber.

    摘要翻译： 提供一种用于电流调节流体pH的微流体装置，包括：第一室，包括第一电极; 第二室，包括第二电极; 第三室，包括第三电极，在第一和第二室之间的金属离子交换膜; 以及在第一或第二室与第三室之间的氢离子交换膜。

    Microfluidic device for electrochemically regulating pH of fluid
    8.
    发明授权
    Microfluidic device for electrochemically regulating pH of fluid 有权
    用于电化学调节流体pH的微流体装置

    公开(公告)号：US08114261B2

    公开(公告)日：2012-02-14

    申请号：US11453538

    申请日：2006-06-15

    申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chin-sung Park ,  Jung-im Han ,  Seung-yeon Yang ,  Shin-i Yoo

    发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chin-sung Park ,  Jung-im Han ,  Seung-yeon Yang ,  Shin-i Yoo

    IPC分类号： B01D61/44

    CPC分类号： B01L3/5027 ,  B01L2200/06

    摘要： A microfluidic device for electrochemically regulating the pH of a fluid includes: an ion-exchange material; an anode chamber having a surface defined by a surface of the ion-exchange material and an anode electrode disposed along an edge of the surface of the anode chamber; and a cathode chamber having a surface defined by an opposite surface of the ion-exchange material and a cathode electrode disposed along an edge of the surface of the cathode chamber.

    摘要翻译： 用于电化学调节流体pH的微流体装置包括：离子交换材料; 具有由所述离子交换材料的表面限定的表面的阳极室和沿着所述阳极室的表面的边缘设置的阳极电极; 以及阴极室，其具有由离子交换材料的相对表面限定的表面和沿着阴极室的表面的边缘设置的阴极电极。

    Method of amplifying nucleic acid from a cell using a nonplanar solid substrate
    9.
    发明授权
    Method of amplifying nucleic acid from a cell using a nonplanar solid substrate 有权
    使用非平面固体底物从细胞中扩增核酸的方法

    公开(公告)号：US07919278B2

    公开(公告)日：2011-04-05

    申请号：US11841107

    申请日：2007-08-20

    申请人： Sung-young Jeong ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Jung-im Han ,  Hun-joo Lee

    发明人： Sung-young Jeong ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Jung-im Han ,  Hun-joo Lee

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/1006 ,  B01L7/52 ,  B01L2300/0636 ,  C12N2533/30 ,  C12Q1/6806

    摘要： Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.

    摘要翻译： 本发明提供一种扩增来自a的核酸的方法，其特征在于：在pH范围为3.0-6.0的液体介质中使含细胞的样品与非平面固体基质接触，以将该细胞附着在固体基质上; 洗涤非平面固体基质以除去未附着于其上的材料; 以及使用来自附着在所述非平面固体底物上的细胞的核酸作为模板样品进行PCR以扩增来自所述细胞的核酸，其中所述接触，洗涤和PCR过程在单个容器中进行。