公开(公告)号：US09952196B2

公开(公告)日：2018-04-24

申请号：US14985986

申请日：2015-12-31

申请人： NATIONAL CHUNG CHENG UNIVERSITY

发明人： Shau-Chun Wang ,  Teh-Sheng Lai ,  Ting-Chou Chang

IPC分类号： G01N33/487 ,  B01D61/44 ,  B01D61/46 ,  G01N21/25 ,  G01N27/447 ,  B01L3/00 ,  G01N1/40 ,  G01N21/552 ,  G01N33/68

CPC分类号： G01N33/487 ,  B01D61/44 ,  B01D61/46 ,  B01D2311/02 ,  B01D2311/2623 ,  B01D2311/2684 ,  B01L3/50 ,  G01N1/4005 ,  G01N21/251 ,  G01N21/554 ,  G01N27/44721 ,  G01N27/44791 ,  G01N33/6827 ,  G01N2001/4011 ,  G01N2201/0221 ,  G01N2201/12 ,  G01N2333/76

摘要： A method for detecting albumin based on a colorimetric assay and a system thereof are disclosed. Gold nanoparticles are added into the sample preparing device having a sample without spectroscopic tags, wherein the sample without spectroscopic tags is formed as the alkaline solution to avoid the interference substances adhering on the gold nanoparticles. The gold nanoparticles are concentrated by using the microfluidic concentrator with the circular ion exchange membrane by applying an external electric field across two electrodes. The image of the concentrated gold nanoparticles is captured by the image capturing device for measuring the saturation intensities of the image, wherein there is a relation between the saturation intensities and the concentration of the albumin in the sample without spectroscopic tags. The concentration of the albumin of the sample without spectroscopic tags is obtained by the relation and the measured saturation intensities.

公开(公告)号：US20120186979A1

公开(公告)日：2012-07-26

申请号：US13312377

申请日：2011-12-06

申请人： Roger Trones ,  Trond Lovli

发明人： Roger Trones ,  Trond Lovli

IPC分类号： C25B7/00 ,  B01D43/00

CPC分类号： G01N1/4005 ,  B01D61/38 ,  G01N2001/4011 ,  G01N2001/4016

摘要： A device for electro membrane extraction has a syringe holder adapted to hold a syringe having an acceptor solution, and a sample vial holder adapted to hold a sample vial having a vial cap, where the vial cap includes an inside funnel to be equipped with a prewetted hollow fiber membrane having a tube like shape sealed at the end opposite the funnel and forming a lumen, and steering guides for at least two electrodes, a first electrode to be immersed in a donor solution placed in the sample vial, a second electrode to be immersed, through the funnel in the vial cap, into the lumen of the hollow fiber membrane, and a positioning device for sliding the first electrode in and out of the donor solution in the sample vial and for sliding the second electrode in and out of the lumen of the hollow fiber membrane.

摘要翻译： 用于电膜提取的装置具有适于保持具有受体溶液的注射器的注射器保持器和适于保持具有小瓶盖的样品瓶的样品瓶保持架，其中小瓶盖包括内漏斗以配备预润湿 中空纤维膜，其具有在与漏斗相对的端部密封并形成内腔的管状形状，以及用于至少两个电极的转向引导件，浸没在放置在样品瓶中的供体溶液中的第一电极，第二电极 通过小瓶盖中的漏斗浸入中空纤维膜的内腔中;以及定位装置，用于将第一电极滑入和取出样品瓶中的供体溶液，并将第二电极滑入和移出 中空纤维膜的内腔。

公开(公告)号：US20060266650A1

公开(公告)日：2006-11-30

申请号：US11440872

申请日：2006-05-25

申请人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

发明人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

IPC分类号： B01D61/42

CPC分类号： B01D61/422 ,  B01D61/46 ,  B01L3/5027 ,  G01N2001/4011

摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.

摘要翻译： 提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。 该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。 即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

公开(公告)号：US20050284762A1

公开(公告)日：2005-12-29

申请号：US11155150

申请日：2005-06-17

申请人： Juan Astorga-Wells ,  Sang-Ryoul Park ,  Harold Swerdlow ,  Gerald Jesson ,  Ulf Lindberg ,  Tomas Bergman ,  Hans Jornvall ,  Mats Jonsson

发明人： Juan Astorga-Wells ,  Sang-Ryoul Park ,  Harold Swerdlow ,  Gerald Jesson ,  Ulf Lindberg ,  Tomas Bergman ,  Hans Jornvall ,  Mats Jonsson

IPC分类号： B01D57/02 ,  B01L3/00 ,  B81B3/00 ,  G01N20060101 ,  G01N1/34 ,  G01N27/404 ,  G01N27/447

CPC分类号： G01N1/405 ,  B01L3/502753 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N27/44769 ,  G01N2001/4011

摘要： The invention relates to a method for capturing charged molecules of interest traveling in an electrolyte flow stream through an electrically non-conductive channel, comprising at least one anode and at least one cathode individually separated from said channel, but in electrical contact with said flow stream, by a conductive ion selective semi-permeable membrane. Said membrane interferes with the normal migration of ions towards its respective electrode, generating at least two zones of different electric field. Balance between hydrodynamic and electrical forces captures certain ions into the flow stream. it also relates to a device for performing the method.

摘要翻译： 本发明涉及一种用于捕获通过非导电通道在电解液流中行进的感兴趣的带电分子的方法，该方法包括至少一个阳极和至少一个与所述通道分离的阳极，但是与所述流动流 ，通过导电离子选择性半透膜。 所述膜干扰离子向其相应电极的正常迁移，产生至少两个不同电场的区域。 流体动力学和电力之间的平衡将某些离子捕获到流动流中。 它还涉及一种用于执行该方法的装置。

公开(公告)号：US20180280832A1

公开(公告)日：2018-10-04

申请号：US15943592

申请日：2018-04-02

申请人： ChromoLogic LLC

发明人： Naresh Menon ,  Peter Bui ,  Cheryl Tan

IPC分类号： B01D15/36 ,  G01N1/30 ,  G01N1/40 ,  B01D71/16 ,  B01J20/286

CPC分类号： B01D15/362 ,  B01D63/087 ,  B01D69/12 ,  B01D71/027 ,  B01D71/10 ,  B01D71/16 ,  B01D71/28 ,  B01D2313/04 ,  B01D2313/105 ,  B01J20/286 ,  B01L2300/0681 ,  G01N1/30 ,  G01N1/38 ,  G01N1/4005 ,  G01N1/405 ,  G01N2001/2826 ,  G01N2001/381 ,  G01N2001/383 ,  G01N2001/4011 ,  G01N2001/4016

摘要： An apparatus is disclosed for separating and preserving biomolecules of a biological fluid sample. The apparatus includes an assembly having sides forming a hollow shape having a first opening at one end and second opening at the opposite end, a sample mixing chamber positioned adjacent the first opening within the assembly, the sample mixing chamber from which a flow of the biological fluid sample is actuated in a direction from the sample mixing chamber to the first matrix layer, the sample mixing chamber being in a direction downstream of the first opening, a first valve positioned between the sample mixing chamber and the first matrix layer, the first valve configured to control the flow to the first matrix layer, a first input in fluid communication with the sample mixing chamber and positioned upstream of the first valve, a second input positioned between the first matrix layer and the second matrix layer, and a second valve positioned between the second matrix layer and the second opening, the second valve configured to control the flow to the second matrix layer.

公开(公告)号：US20170138824A1

公开(公告)日：2017-05-18

申请号：US15322527

申请日：2014-07-02

申请人： SHIMADZU CORPORATION

发明人： Nobuhiro HANAFUSA ,  Masamitsu SHIKATA

IPC分类号： G01N1/10 ,  G01N1/34 ,  G01N30/06 ,  G01N1/14

CPC分类号： G01N1/10 ,  B01L3/502 ,  B01L2200/0631 ,  G01N1/14 ,  G01N1/34 ,  G01N1/4005 ,  G01N30/06 ,  G01N35/0099 ,  G01N35/025 ,  G01N2001/4011 ,  G01N2035/00485 ,  G01N2035/00534 ,  G01N2035/0427 ,  G01N2035/0441 ,  G01N2035/0443

摘要： A preprocessing apparatus uses preprocessing kits prepared for samples, respectively, sets the preprocessing kits in plural processing ports provided so as to correspond to preprocessing items, and executes a predetermined preprocessing item. A controller which controls operations of a carrying mechanism holding and carrying the preprocessing kits, and processing parts executing the preprocessing items in the respective processing ports, includes a processing-state control part, a random access part, and a preprocessing part. The random access part is configured to check availability of a preprocessing port corresponding to a preprocessing item to be executed on a sample contained in a preprocessing kit, and set the preprocessing kit in a processing port if the processing port is available as a processing port corresponding to the preprocessing item. The preprocessing part executes a corresponding preprocessing item on a sample in a preprocessing kit when the preprocessing kit is set in the processing port.

公开(公告)号：US20120231486A1

公开(公告)日：2012-09-13

申请号：US13510099

申请日：2010-11-16

申请人： Thorleif Lavold ,  Juan Astorga Wells

发明人： Thorleif Lavold ,  Juan Astorga Wells

IPC分类号： G01N27/72 ,  G01N31/00 ,  C12M1/34

CPC分类号： C07K1/13 ,  B01D15/361 ,  G01N27/26 ,  G01N30/00 ,  G01N30/06 ,  G01N30/7266 ,  G01N30/96 ,  G01N2001/4011 ,  G01N2001/4016 ,  G01N2030/067 ,  G01N2030/146 ,  G06F19/703 ,  H01J49/00 ,  H01J49/0018 ,  H01J49/04 ,  H01J49/0431 ,  H01J49/165 ,  Y10T436/24

摘要： Apparatus and methods to perform hydrogen/deuterium exchange using semipermeable membranes are described. The system has two channels separated by a semipermeable membrane. One channel comprises a flow carrying the analyte of interest, and the second channel comprises a solution comprising a deuterated solvent (e.g. deuterium oxide). The system does not require an external electric field gradient across the membrane to perform the hydrogen-deuterium exchange procedure. The present invention facilitates sample and reagent handling as well as simplifies manufacture of devices and/or instrumentation related to deuterium exchange.Further described is a chemical analysation device for analysing chemical compositions and/or compounds, and a method for analysing chemical compounds and a computer program product for inducing a computer to perform steps in the method. Also described is a method for analyzing interactions between analytes and charged molecules, and calculating binding coefficients of the analytes with respect to the charged molecules.

摘要翻译： 描述了使用半透膜进行氢/氘交换的装置和方法。 该系统具有由半透膜隔开的两个通道。 一个通道包含携带目的分析物的流，第二通道包括含有氘代溶剂（例如氧化氘）的溶液。 该系统不需要跨膜的外部电场梯度来执行氢 - 氘交换过程。 本发明便于样品和试剂处理，并且简化了与氘交换相关的装置和/或仪器的制造。 进一步描述了用于分析化学组合物和/或化合物的化学分析装置，以及用于分析化合物的方法和用于诱导计算机执行该方法中的步骤的计算机程序产品。 还描述了一种用于分析分析物和带电分子之间的相互作用并计算分析物相对于带电分子的结合系数的方法。

公开(公告)号：US07964411B2

公开(公告)日：2011-06-21

申请号：US11762000

申请日：2007-06-12

申请人： Purnendu Dasgupta ,  Masaki Takeuchi ,  Kannan Srinivasan

发明人： Purnendu Dasgupta ,  Masaki Takeuchi ,  Kannan Srinivasan

IPC分类号： G01N30/02

CPC分类号： G01N30/08 ,  G01N1/4005 ,  G01N1/4022 ,  G01N30/96 ,  G01N2001/4011 ,  G01N2001/4027 ,  G01N2030/146 ,  Y10T436/255

摘要： A sample concentrator for concentrating analytes in a solvent-containing liquid sample stream, including concentrator housing having a sample stream flow channel and a gas stream flow channel having an inlet and an outlet, a heater for gas in the gas stream conduit, and a hydrophilic ion exchange or non-ionic membrane barrier separating said gas stream flow channel and said sample stream flow channel. Solvent is evaporated from the liquid sample stream in said sample stream flow channel in or at the interface with said membrane, when the gas stream is at an elevated temperature. A regeneration step is used to regenerate the ion exchange membrane barrier.

摘要翻译： 用于将分析物浓缩在含溶剂的液体样品流中的样品浓缩器，包括具有样品流流动通道的浓缩器壳体和具有入口和出口的气流流动通道，气流导管中的气体加热器，以及亲水 分离所述气流流动通道和所述样品流流动通道的离子交换或非离子膜屏障。 当气流处于升高的温度时，溶剂在所述样品流流动通道中或与所述膜的界面处从液体样品流中蒸发。 再生步骤用于再生离子交换膜屏障。

公开(公告)号：US07722751B2

公开(公告)日：2010-05-25

申请号：US11440872

申请日：2006-05-25

申请人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

发明人： Jung-im Han ,  Young-sun Lee ,  Joon-ho Kim

IPC分类号： B01D61/48

CPC分类号： B01D61/422 ,  B01D61/46 ,  B01L3/5027 ,  G01N2001/4011

摘要： An apparatus for regulating salt concentration, a lab-on-a-chip including the same and a method of regulating salt concentration using the apparatus are provided. The apparatus includes: a reaction chamber that is defined by a cation exchange membrane and an anion exchange membrane and is selected from the group consisting of a biomolecule extraction chamber, an amplification chamber, a hybridization chamber, and a detection chamber; a first electrode chamber that is defined by the anion exchange membrane and a first electrode and includes an ion exchange medium; and a second electrode chamber that is defined by the cation exchange membrane and a second electrode and includes an ion exchange medium. Even without injecting solutions with different salt concentrations into the reaction chamber by operating pumps and valves for each operation stage, the salt concentration can be reversibly regulated in situ by adjusting the polarity, intensity and application time of a voltage.

摘要翻译： 提供了一种调节盐浓度的装置，包括该盐浓度的实验室，以及使用该装置调节盐浓度的方法。 该装置包括：由阳离子交换膜和阴离子交换膜限定的反应室，其选自生物分子提取室，扩增室，杂交室和检测室; 由阴离子交换膜和第一电极限定并包括离子交换介质的第一电极室; 以及由阳离子交换膜和第二电极限定并包括离子交换介质的第二电极室。 即使在每个操作阶段通过操作泵和阀都不会将具有不同盐浓度的溶液注入反应室中，可以通过调节电压的极性，强度和施加时间来原位调节盐浓度。

公开(公告)号：US20180259433A1

公开(公告)日：2018-09-13

申请号：US15756965

申请日：2015-10-23

申请人： Hewlett-Packard Development Company, L.P.

发明人： Viktor Shkolnikov ,  Anita Rogacs

IPC分类号： G01N1/40 ,  G01N21/65

CPC分类号： G01N1/4005 ,  B01D61/38 ,  B01D2313/345 ,  G01N21/658 ,  G01N2001/4011 ,  G01N2001/4038

摘要： A target analyte is extracted out of a sample fluid in a sample fluid passage by diffusing the target analyte through a supported liquid membrane to a product fluid passage. Extraction of the target analyte is accelerated by applying an electric field across and perpendicular to the supported liquid membrane with electrodes. Passage of selected ions across an exchange membrane extending between one of the electrodes and the supported liquid membrane is inhibited.