公开(公告)号：US20240117298A1

公开(公告)日：2024-04-11

申请号：US18266625

申请日：2021-12-16

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Naoaki TAOKA ,  Yoshihiro TOYA ,  Hiroshi SHIMIZU ,  Ryutaro KAWAI

IPC: C12N1/20 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/48 ,  C12N9/88 ,  C12N9/90

CPC classification number: C12N1/205 ,  C12N9/0051 ,  C12N9/1029 ,  C12N9/485 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12Y108/01007 ,  C12Y203/0103 ,  C12Y304/11004 ,  C12Y304/19013 ,  C12Y401/99001 ,  C12Y603/02002 ,  C12Y603/02003

Abstract: One or more embodiments of the present invention provide a microbial strain with improved productivity of γ-glutamylcysteine, bis-γ-glutamylcystine, γ-glutamylcystine, reduced glutathione, and/or oxidized glutathione. Such microbial strain has disruption of [1] a gene encoding γ-glutamyltransferase and [2] a gene encoding phosphoglycerate mutase and enhanced expression of [3] a gene encoding glutamate-cysteine ligase and/or a gene encoding glutathione synthetase or [4] a gene encoding bifunctional glutathione synthetase. This invention also discloses a method for producing the substances mentioned above via culture of the microbial strain.