公开(公告)号：US20230304061A1

公开(公告)日：2023-09-28

申请号：US17918857

申请日：2021-04-23

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Nobuhiko DOJUN ,  Misato MATSUI ,  Naoaki TAOKA

IPC: C12P21/02 ,  C12N9/10 ,  C12N9/48 ,  C12N9/02 ,  C12N9/88

CPC classification number: C12P21/02 ,  C12N9/0051 ,  C12N9/1029 ,  C12N9/485 ,  C12N9/88 ,  C12Y108/01007 ,  C12Y203/0103 ,  C12Y304/11004 ,  C12Y304/19013 ,  C12Y401/99001

Abstract: This invention is intended to improve glutathione productivity by fermentation using microorganisms. This invention relates to a microbial strain capable of overexpression of γ-glutamylcysteine, bis-γ-glutamylcystine, γ-glutamylcystine, reduced glutathione, and/or oxidized glutathione in which the expression level of a gene encoding serine-O-acetyltransferase (EC:2.3.1.30) is enhanced and a method involving the use of such microbial strain.

公开(公告)号：US20220290203A1

公开(公告)日：2022-09-15

申请号：US17749857

申请日：2022-05-20

Applicant: KANEKA CORPORATION

Inventor： Misato MATSUI ,  Shingo KOBAYASHI ,  Naoaki TAOKA

IPC: C12P21/02 ,  C12P13/00 ,  C12N9/10 ,  C12N9/02 ,  C12N9/00 ,  C12N9/48

Abstract: The present disclosure concerns a method for producing peptides such as glutathione and a microorganism that can be used for such method. One or more embodiments of the first aspect of the present disclosure concern a method for producing peptides such as glutathione comprising culturing a prokaryotic microbial strain in which the expression levels of one or more genes selected from among the gshA gene, the gshB gene, and the gshF gene are enhanced, compared with the expression levels thereof in the wild-type strain thereof in a medium in which the total concentration of cysteine and cystine is 0.5 g/l or lower. The second aspect of the present disclosure concerns a microorganism comprising disruptions of the γ-glutamyltransferase gene and the glutathione reductase gene and exhibiting the enhanced expression levels of the gshA gene and the gshB or gshF gene.

公开(公告)号：US20170198313A1

公开(公告)日：2017-07-13

申请号：US15129726

申请日：2015-03-27

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Rina AOKI ,  Tetsuya MINAMI ,  Hisashi ARIKAWA ,  Keiji MATSUMOTO

IPC: C12P7/62 ,  C12N9/88 ,  C12N9/10

CPC classification number: C12P7/625 ,  C08G63/06 ,  C08L101/16 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/09 ,  C12Y203/01 ,  C12Y402/01017

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.

公开(公告)号：US20210254028A1

公开(公告)日：2021-08-19

申请号：US17252086

申请日：2019-06-12

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Shunsuke SATO ,  Naoaki TAOKA

IPC: C12N9/10 ,  C12N15/52 ,  C12P7/62

Abstract: Provided is a mutant polyhydroxyalkanoate synthetase having an amino acid sequence which has 85% or more sequence identity with the amino acid sequence of SEQ ID NO: 1 and which contains a mutation of substitution of at least one of the amino acids at the 27th to 33rd, 39th, 56th, 106th, 129th, 144th, 165th, 170th and 172nd to 187th positions from the N-terminus of the amino acid sequence of SEQ ID NO: 1 with another amino acid.

公开(公告)号：US20170347648A1

公开(公告)日：2017-12-07

申请号：US15658799

申请日：2017-07-25

Applicant: NATIONAL RESEARCH AND DEVELOPMENT AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION ,  KANEKA CORPORATION

Inventor： Hitoshi NAKAMURA ,  Koichi SUZAKI ,  Takahiko SUGAYA ,  Shingo KOBAYASHI

IPC: A01N37/36 ,  A01M17/00 ,  A01N25/02 ,  A01N25/12

CPC classification number: A01N37/36 ,  A01M17/00 ,  A01N25/02 ,  A01N25/12 ,  A01N61/00

Abstract: Disclosed herein is a soil-borne disease control method capable of simply suppressing a soil-borne pathogen. The method for controlling a soil-borne disease includes applying at least one polyhydroxyalkanoic acid having a structure represented by the following formula (1) to soil: [—CHR—CH2—CO—O—]  (1) (wherein R is an alkyl group represented by CnH2n+1 and n is an integer of 1 or more but 15 or less).

公开(公告)号：US20180346943A1

公开(公告)日：2018-12-06

申请号：US16100688

申请日：2018-08-10

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Tetsuya FUJIKI

IPC: C12P7/62 ,  C08G63/06 ,  C08G63/91

Abstract: The present invention provides a novel polyhydroxyalkanoic acid having a functional group at a terminal carboxy group, which being capable of being chemically modified and easily controlled in reaction, and a method for production the same. The present polyhydroxyalkanoic acid includes a specific alkyne group, alkene group, thiol group, azide group or allyl group introduced at a terminal carboxy group. Further, the present production method comprises culturing a microorganism capable of producing a polyhydroxyalkanoic acid with use of an alcohol having an alkyne group, an alkene group, a thiol group, an azide group or an allyl group.

公开(公告)号：US20180305722A1

公开(公告)日：2018-10-25

申请号：US15763323

申请日：2016-09-15

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Hitoshi SASHIWA ,  Tetsuya FUJIKI

IPC: C12P7/62 ,  C08G63/06 ,  C12N15/74 ,  C12N1/20

CPC classification number: C12P7/625 ,  C08G63/06 ,  C12N1/20 ,  C12N15/09 ,  C12N15/74 ,  C12P7/62

Abstract: An object of the invention is to improve the crystallization speed of a PHA copolymer which is to be slowly crystallized, and improve the melt workability and productivity. A microorganism is used which has genes encoding two or more different PHA synthases derived from the genus Aeromonas. The genes encoding the PHA synthases derived from the genus Aeromonas preferably include genes encoding at least two PHA synthases which are capable of synthesizing a copolymer PHA including, as monomer unit species, 3-hydroxybutyric acid and 3-hydroxyhexanoic acid, and which are different in substrate specificity toward 3-hydroxyhexanoic acid from each other. When this microorganism is cultured, a PHA mixture can be produced which includes three or more PHA species different in melting point from each other.

公开(公告)号：US20240117298A1

公开(公告)日：2024-04-11

申请号：US18266625

申请日：2021-12-16

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Naoaki TAOKA ,  Yoshihiro TOYA ,  Hiroshi SHIMIZU ,  Ryutaro KAWAI

IPC: C12N1/20 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/48 ,  C12N9/88 ,  C12N9/90

CPC classification number: C12N1/205 ,  C12N9/0051 ,  C12N9/1029 ,  C12N9/485 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12Y108/01007 ,  C12Y203/0103 ,  C12Y304/11004 ,  C12Y304/19013 ,  C12Y401/99001 ,  C12Y603/02002 ,  C12Y603/02003

Abstract: One or more embodiments of the present invention provide a microbial strain with improved productivity of γ-glutamylcysteine, bis-γ-glutamylcystine, γ-glutamylcystine, reduced glutathione, and/or oxidized glutathione. Such microbial strain has disruption of [1] a gene encoding γ-glutamyltransferase and [2] a gene encoding phosphoglycerate mutase and enhanced expression of [3] a gene encoding glutamate-cysteine ligase and/or a gene encoding glutathione synthetase or [4] a gene encoding bifunctional glutathione synthetase. This invention also discloses a method for producing the substances mentioned above via culture of the microbial strain.

公开(公告)号：US20210214645A1

公开(公告)日：2021-07-15

申请号：US17058932

申请日：2019-05-27

Applicant: KANEKA CORPORATION

Inventor： Takafumi IGARI ,  Wanying LEE ,  Shingo KOBAYASHI

IPC: C11C1/10 ,  C12P7/62

Abstract: A free fatty acid-containing substance, which is a waste liquid or by-product obtained in a process for producing palm oil or which is a hydrolysate of the waste liquid or by-product, is subjected to distillation treatment to obtain a free fatty acid fraction satisfying the requirements (i), (ii) and (iii), and a polyhydroxyalkanoate-producing microorganism is cultured in a culture solution containing the free fatty acid fraction. (i) The content of squalene is 0.05 wt % or less. (ii) The ratio of a peak area of trimethylindene to a peak area of a naphthalene-d8 standard is 110 or less in first-dimensional dynamic headspace-GC/TOFMS analysis. (iii) The ratio of a peak area of butyric acid to a peak area of a naphthalene-d8 standard is less than 10 in first-dimensional dynamic headspace-GC/TOFMS analysis.

公开(公告)号：US20200056214A1

公开(公告)日：2020-02-20

申请号：US16670042

申请日：2019-10-31

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Rina AOKI ,  Tetsuya MINAMI ,  Hisashi ARIKAWA ,  Keiji MATSUMOTO

IPC: C12P7/62 ,  C12N9/88 ,  C12N9/10 ,  C08G63/06

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.