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1.发明公开公开(公告)号：US20240117298A1
公开(公告)日：2024-04-11
申请号：US18266625
申请日：2021-12-16
Applicant: KANEKA CORPORATION
Inventor： Shingo KOBAYASHI , Naoaki TAOKA , Yoshihiro TOYA , Hiroshi SHIMIZU , Ryutaro KAWAI
CPC classification number: C12N1/205 , C12N9/0051 , C12N9/1029 , C12N9/485 , C12N9/88 , C12N9/90 , C12N9/93 , C12Y108/01007 , C12Y203/0103 , C12Y304/11004 , C12Y304/19013 , C12Y401/99001 , C12Y603/02002 , C12Y603/02003
Abstract: One or more embodiments of the present invention provide a microbial strain with improved productivity of γ-glutamylcysteine, bis-γ-glutamylcystine, γ-glutamylcystine, reduced glutathione, and/or oxidized glutathione. Such microbial strain has disruption of [1] a gene encoding γ-glutamyltransferase and [2] a gene encoding phosphoglycerate mutase and enhanced expression of [3] a gene encoding glutamate-cysteine ligase and/or a gene encoding glutathione synthetase or [4] a gene encoding bifunctional glutathione synthetase. This invention also discloses a method for producing the substances mentioned above via culture of the microbial strain.
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公开(公告)号：US20220298518A1
公开(公告)日：2022-09-22
申请号：US17641730
申请日：2020-09-02
Applicant: KANEKA CORPORATION
Inventor： Haruyasu HAMADA , Yozo NAGIRA , Akira ENDO , Naoaki TAOKA
IPC: C12N15/82
Abstract: A method for transforming a plant includes exposing a shoot apex of an axillary bud of a plant body, and introducing, into the shoot apex, a microparticle coated with at least one kind of nucleic acid.
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Patent Agency Ranking
公开(公告)号：US20130079525A1
公开(公告)日：2013-03-28
申请号：US13682840
申请日：2012-11-21
Applicant: KANEKA CORPORATION
Inventor： Kohei MORI , Masutoshi NOJIRI , Akira NISHIYAMA , Naoaki TAOKA
IPC: C07D211/56 , C07D211/60
CPC classification number: C07D211/60 , C07B57/00 , C07D211/56 , C12P17/12 , C12P41/006
Abstract: The present invention relates to a method for producing an optically active 3-aminopiperidine or salt thereof. In the method, a racemic nipecotamide is stereoselectively hydrolyzed to obtain an optically active nipecotamide and an optically active nipecotic acid in the presence of an enzyme source derived from an organism, and then the optically active nipecotamide is derived into an optically active aminopiperidine or salt thereof by aroylation, Hofmann rearrangement, deprotection of the amino group and further deprotection; or the optically active nipecotamide is derived into an optically active aminopiperidine or salt thereof by selective protection with BOC, Hofmann rearrangement and further deprotection. It is possible by the present invention to produce an optically active 3-aminopiperidine or salt thereof useful as a pharmaceutical intermediate from an inexpensive and easily available starting material by easy method applicable to industrial manufacturing.
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4.发明公开公开(公告)号：US20230304061A1
公开(公告)日：2023-09-28
申请号：US17918857
申请日：2021-04-23
Applicant: KANEKA CORPORATION
Inventor： Shingo KOBAYASHI , Nobuhiko DOJUN , Misato MATSUI , Naoaki TAOKA
CPC classification number: C12P21/02 , C12N9/0051 , C12N9/1029 , C12N9/485 , C12N9/88 , C12Y108/01007 , C12Y203/0103 , C12Y304/11004 , C12Y304/19013 , C12Y401/99001
Abstract: This invention is intended to improve glutathione productivity by fermentation using microorganisms. This invention relates to a microbial strain capable of overexpression of γ-glutamylcysteine, bis-γ-glutamylcystine, γ-glutamylcystine, reduced glutathione, and/or oxidized glutathione in which the expression level of a gene encoding serine-O-acetyltransferase (EC:2.3.1.30) is enhanced and a method involving the use of such microbial strain.
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公开(公告)号：US20220290203A1
公开(公告)日：2022-09-15
申请号：US17749857
申请日：2022-05-20
Applicant: KANEKA CORPORATION
Inventor： Misato MATSUI , Shingo KOBAYASHI , Naoaki TAOKA
Abstract: The present disclosure concerns a method for producing peptides such as glutathione and a microorganism that can be used for such method. One or more embodiments of the first aspect of the present disclosure concern a method for producing peptides such as glutathione comprising culturing a prokaryotic microbial strain in which the expression levels of one or more genes selected from among the gshA gene, the gshB gene, and the gshF gene are enhanced, compared with the expression levels thereof in the wild-type strain thereof in a medium in which the total concentration of cysteine and cystine is 0.5 g/l or lower. The second aspect of the present disclosure concerns a microorganism comprising disruptions of the γ-glutamyltransferase gene and the glutathione reductase gene and exhibiting the enhanced expression levels of the gshA gene and the gshB or gshF gene.
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公开(公告)号：US20210254028A1
公开(公告)日：2021-08-19
申请号：US17252086
申请日：2019-06-12
Applicant: KANEKA CORPORATION
Inventor： Shingo KOBAYASHI , Shunsuke SATO , Naoaki TAOKA
Abstract: Provided is a mutant polyhydroxyalkanoate synthetase having an amino acid sequence which has 85% or more sequence identity with the amino acid sequence of SEQ ID NO: 1 and which contains a mutation of substitution of at least one of the amino acids at the 27th to 33rd, 39th, 56th, 106th, 129th, 144th, 165th, 170th and 172nd to 187th positions from the N-terminus of the amino acid sequence of SEQ ID NO: 1 with another amino acid.
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公开(公告)号：US20170137833A1
公开(公告)日：2017-05-18
申请号：US15311435
申请日：2015-05-15
Applicant: KANEKA CORPORATION
Inventor： Haruyasu HAMADA , Yozo NAGIRA , Naoaki TAOKA , Ryozo IMAI , Mineo KOJIMA , Ryuji MIKI
IPC: C12N15/82
CPC classification number: C12N15/8205
Abstract: It is an object solved by the present invention to provide an Agrobacterium with increased gene targeting efficiency. The present invention provides an Agrobacterium having a T-DNA nuclear translocation ability equivalent to that of a wild-type Agrobacterium and also having a T-DNA chromosomal insertion ability that is lost or reduced in comparison to a wild-type Agrobacterium.
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