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公开(公告)号：US11208675B2
公开(公告)日：2021-12-28
申请号：US16317209
申请日：2017-07-13
申请人： Kikkoman Corporation
摘要： Provided is a compound capable of accelerating the enzymatic reaction catalyzed by peroxidase. The present invention provides a peroxidase reaction accelerating agent comprising a compound represented by formula (I) and a method for measuring hydrogen peroxide using the same.
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公开(公告)号：US20190316174A1
公开(公告)日：2019-10-17
申请号：US16317216
申请日：2017-07-13
申请人： Kikkoman Corporation
IPC分类号： C12Q1/26 , C07C245/10 , C07D231/46
摘要： Provided is a compound which accelerates the enzymatic reaction catalyzed by an oxidase. The present invention provides an oxidase reaction accelerating agent comprising a compound represented by formula (I) and a method using the same.
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公开(公告)号：US09701949B2
公开(公告)日：2017-07-11
申请号：US14369192
申请日：2012-12-27
申请人： KIKKOMAN CORPORATION
IPC分类号： C12N9/06
CPC分类号： C12N9/0032 , C12Y105/03
摘要： An amadoriase having a substitution or a deletion of one or more amino acid residues at positions corresponding to amino acids selected from the group consisting of three amino acid residues from the carboxyl terminal and amino acids at positions 151, 43, 53, 267, 350, 185, 196, 299 and 323 in the amino acid sequence of amadoriase derived from the Coniochaeta species indicated in SEQ ID NO: 1. The amadoriase having a heat resistance which is superior to that of a conventional amadoriase.
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公开(公告)号：US11549134B2
公开(公告)日：2023-01-10
申请号：US16832712
申请日：2020-03-27
申请人： Kikkoman Corporation
摘要： Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.
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公开(公告)号：US10934530B2
公开(公告)日：2021-03-02
申请号：US15563054
申请日：2016-04-04
申请人： Kikkoman Corporation
摘要： This invention provides an amadoriase having improved specific activity on a glycated substrate, compared with conventional amadoriase. Provided is an amadoriase comprising a substitution of the amino acid at the position corresponding to position 64 of the amino acid sequence as shown in SEQ ID NO: 1 with an amino acid selected from the group consisting of glycine, serine, methionine, leucine, threonine, valine, and isoleucine, a method for measurement of HbA1c, and a reagent kit for measurement of HbA1c using such amadoriase. Such method and kit for measurement enable rapid, simple, and accurate quantification of HbA1c.
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公开(公告)号：US20190119715A1
公开(公告)日：2019-04-25
申请号：US16094924
申请日：2017-04-21
申请人： Kikkoman Corporation
IPC分类号： C12Q1/00 , C12N9/02 , G01N27/327
摘要： This invention provides an HbA1c dehydrogenase that is capable of directly acting on hemoglobin A1c and is less likely to be influenced by oxygen concentration and a method for measurement and a kit of assay reagents using such HbA1c dehydrogenase. The HbA1c dehydrogenase having dehydrogenase activity and capable of directly acting on HbA1c is obtained by substitution of one or more amino acid residues at positions corresponding to positions 280, 269, 54, 241, and 267 of the amadoriase that is capable of directly acting on hemoglobin A1c and is derived from, for example, the genus Coniochaeta. This invention also provides a method for measurement of HbA1c, a kit of assay reagents, and a sensor using such HbA1c dehydrogenase. Such HbA1c dehydrogenase is capable of directly acting on hemoglobin A1c and has lowered oxidase activity and/or enhanced dehydrogenase activity. This not only eliminates the need for treatment of hemoglobin A1c with a protease but also enables the use of an electron mediator in the measurement of HbA1c, thereby reducing effects due to oxygen concentration, and enables HbA1c measured with high sensitivity.
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7.发明授权公开(公告)号：US09493814B2
公开(公告)日：2016-11-15
申请号：US14355326
申请日：2012-11-01
申请人： KIKKOMAN CORPORATION
CPC分类号： C12Q1/54 , C12N9/0006 , C12Q1/006 , C12Q1/32
摘要： A flavin-binding glucose dehydrogenase having high substrate specificity for D-glucose and decreased reactivity to D-xylose and/or maltose. More specifically, a flavin-binding glucose dehydrogenase having one or more amino acid substitutions at a position corresponding to position 78, position 79, position 81, position 121, position 122, position 123, position 569 and position 612 of Mucor-derived flavin-binding glucose dehydrogenase. The flavin-binding glucose dehydrogenase enables D-glucose to be measured accurately without being susceptible to the effects of the presence of D-xylose and/or maltose, even under conditions of mounting a large amount of an enzyme such as in glucose sensors.
摘要翻译： 对D-葡萄糖具有高底物特异性并降低对D-木糖和/或麦芽糖的反应性的黄素结合性葡萄糖脱氢酶。 更具体地，在对应于来自毛孔的黄素衍生物的位置78，位置79，位置81，位置121，位置122，位置123，位置569和位置612的位置具有一个或多个氨基酸取代的黄素结合葡萄糖脱氢酶， 结合葡萄糖脱氢酶。 黄素结合葡萄糖脱氢酶能够准确测量D-葡萄糖，即使在安装大量酶如葡萄糖传感器的条件下也不会受到D-木糖和/或麦芽糖的影响的影响。
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公开(公告)号：US20220396822A1
公开(公告)日：2022-12-15
申请号：US17638837
申请日：2020-08-21
申请人： Kikkoman Corporation
发明人： Kazuya Marushima , Yi Hsin Chen , Dian Kartikasari Lianto , Takuya Sato , Atsushi Ichiyanagi , Yasuko Araki
摘要： Provided is a method for measuring pentosidine in a specimen, the measurement method comprising the steps of: degrading the specimen with an amino acid degrading enzyme; contacting the specimen after the degradation step with a protein having activity that oxidatively degrades pentosidine; and detecting change resulting from the contact, wherein the amino acid degrading enzyme and the protein having activity that oxidatively degrades pentosidine are different from each other.
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公开(公告)号：US11111517B2
公开(公告)日：2021-09-07
申请号：US16094924
申请日：2017-04-21
申请人： Kikkoman Corporation
IPC分类号： C12Q1/32 , C12N15/09 , C12M1/40 , G01N33/483 , G01N27/416 , G01N33/49 , G01N27/327 , C12N9/88 , C12Q1/00 , G01N33/72 , C12N9/02
摘要： This invention provides an HbA1c dehydrogenase that is capable of directly acting on hemoglobin A1c and is less likely to be influenced by oxygen concentration and a method for measurement and a kit of assay reagents using such HbA1c dehydrogenase. The HbA1c dehydrogenase having dehydrogenase activity and capable of directly acting on HbA1c is obtained by substitution of one or more amino acid residues at positions corresponding to positions 280, 269, 54, 241, and 267 of the amadoriase that is capable of directly acting on hemoglobin A1c and is derived from, for example, the genus Coniochaeta. This invention also provides a method for measurement of HbA1c, a kit of assay reagents, and a sensor using such HbA1c dehydrogenase. Such HbA1c dehydrogenase is capable of directly acting on hemoglobin A1c and has lowered oxidase activity and/or enhanced dehydrogenase activity. This not only eliminates the need for treatment of hemoglobin A1c with a protease but also enables the use of an electron mediator in the measurement of HbA1c, thereby reducing effects due to oxygen concentration, and enables HbA1c measured with high sensitivity.
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公开(公告)号：US11078517B2
公开(公告)日：2021-08-03
申请号：US15031037
申请日：2014-10-24
申请人： Kikkoman Corporation
摘要： This invention provides an amadoriase that acts on the β-chain of hemoglobin A1c (HbA1c) and generates hydrogen peroxide, a method for measurement of HbA1c using such amadoriase, and a reagent kit for measurement of HbA1c using such amadoriase. The method for measurement of HbA1c involves the use of an amadoriase that has specific activity of 0.1 U/mg or greater to αF6P and oxidizes the HbA1c β-chain amino terminus so as to generate hydrogen peroxide, and the reagent kit for measurement of HbA1c comprises such amadoriase. The method and the kit for measurement of HbA1c enable quantification of HbA1c to be performed rapidly, simply, and accurately.
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