公开(公告)号：US20180275134A1

公开(公告)日：2018-09-27

申请号：US15544795

申请日：2016-01-15

Applicant: KONICA MINOLTA, INC.

Inventor： Takeshi ISODA ,  Naoko FURUSAWA ,  Yasuyuki MOTOKUI ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE

IPC: G01N33/58 ,  G01N33/68

Abstract: There is provided a biological substance quantitation method of quantitating a biological substance in a sample stained with a staining reagent including a fluorescent particle encapsulating a fluorescent substance, based on a fluorescence of the fluorescent substance. The method includes inputting a fluorescent image representing expression of the biological substance in the sample by a fluorescent bright spot; and quantitating an expression amount of the biological substance based on a fluorescence of the fluorescent bright spot. The biological substance is a nucleoprotein expressed at a cell nucleus. The fluorescent particle binds to the biological substance through a primary antibody which is directed against the biological substance as an antigen.

公开(公告)号：US20240282405A1

公开(公告)日：2024-08-22

申请号：US18567367

申请日：2022-06-23

Applicant: KONICA MINOLTA, INC.

Inventor： Ken OKAMOTO ,  Takatoshi SUEMATSU ,  Wataru ONODA ,  Atsuro TATSUMI ,  Yuichiro ASAI ,  Naoko FURUSAWA

IPC: G16B15/20 ,  G01N27/414 ,  G16B40/10

CPC classification number: G16B15/20 ,  G01N27/414 ,  G16B40/10

Abstract: An analysis system, a learned model generation device, a discrimination system, an analysis method, a learned model generation method, and a discrimination method can sensitively quantify at least one of an aggregation state or a surface state. An analysis system includes an acquisition unit for acquiring a signal change over time due to interaction between a capture target substance and a capture substance by an electrical detection method or optical detection method, and an analysis unit for analyzing at least one of an aggregation state and a surface state of the capture target substance from the signal change over time acquired by the acquisition unit.

公开(公告)号：US20170205417A1

公开(公告)日：2017-07-20

申请号：US15327782

申请日：2015-07-21

Applicant: KONICA MINOLTA, INC.

Inventor： Takuji AIMIYA ,  Naoko FURUSAWA

IPC: G01N33/574 ,  C07D401/14 ,  G01N33/58

CPC classification number: G01N33/57438 ,  A61K49/00 ,  C07D401/14 ,  G01N33/48 ,  G01N33/582

Abstract: An object of the present invention is to provide a labeling agent which exhibits excellent binding to a target molecule in a bioassay or histological staining method that relates to a compound containing a nitrogen-containing aromatic ring.The labeling agent according to the present invention is a labeling agent having a structure in which a molecular target drug, which is a compound containing a nitrogen atom-containing aromatic ring, is bound with a label via a divalent linking group, wherein one end of the divalent linking group is bound to a carbon atom of the nitrogen atom-containing aromatic ring. The label is preferably a fluorescent substance-integrated nanoparticle, and the nitrogen atom-containing aromatic ring is preferably a pyridine ring.

公开(公告)号：US20170186156A1

公开(公告)日：2017-06-29

申请号：US15325355

申请日：2014-07-11

Applicant: KONICA MINOLTA, INC.

Inventor： Takeshi ISODA ,  Naoko FURUSAWA ,  Yasuhiro WATANABE

IPC: G06T7/00 ,  G06T7/90 ,  G01N33/543 ,  G01N21/64 ,  G01N1/30

CPC classification number: G06T7/0012 ,  G01N1/30 ,  G01N21/64 ,  G01N21/6428 ,  G01N33/543 ,  G01N33/54313 ,  G01N2021/6439 ,  G06T7/90 ,  G06T2207/10061 ,  G06T2207/10064 ,  G06T2207/30024 ,  G06T2207/30242

Abstract: A quantitative determination method of a biological substance in a sample stained with a staining reagent comprising fluorescent particles each encapsulating a fluorescent substance and binding to a biological substance recognizing site. The method comprises inputting a fluorescent image obtained by photographing the sample, extracting a predetermined region from the fluorescent image to calculate an integrated luminance of the predetermined region, and counting the number of fluorescent particles contained in the predetermined region from the integrated luminance and the average luminance per fluorescent particle. The average luminance per fluorescent particle is calculated from a correlation between the number of fluorescent particles counted from an image of the fluorescent particles visualized and the luminance derived from fluorescent light from the fluorescent particles and calculated from a fluorescent image of a region identical to the region taken in the image from which the number of fluorescent particles is counted.