公开(公告)号：US20170186156A1

公开(公告)日：2017-06-29

申请号：US15325355

申请日：2014-07-11

Applicant: KONICA MINOLTA, INC.

Inventor： Takeshi ISODA ,  Naoko FURUSAWA ,  Yasuhiro WATANABE

IPC: G06T7/00 ,  G06T7/90 ,  G01N33/543 ,  G01N21/64 ,  G01N1/30

CPC classification number: G06T7/0012 ,  G01N1/30 ,  G01N21/64 ,  G01N21/6428 ,  G01N33/543 ,  G01N33/54313 ,  G01N2021/6439 ,  G06T7/90 ,  G06T2207/10061 ,  G06T2207/10064 ,  G06T2207/30024 ,  G06T2207/30242

Abstract: A quantitative determination method of a biological substance in a sample stained with a staining reagent comprising fluorescent particles each encapsulating a fluorescent substance and binding to a biological substance recognizing site. The method comprises inputting a fluorescent image obtained by photographing the sample, extracting a predetermined region from the fluorescent image to calculate an integrated luminance of the predetermined region, and counting the number of fluorescent particles contained in the predetermined region from the integrated luminance and the average luminance per fluorescent particle. The average luminance per fluorescent particle is calculated from a correlation between the number of fluorescent particles counted from an image of the fluorescent particles visualized and the luminance derived from fluorescent light from the fluorescent particles and calculated from a fluorescent image of a region identical to the region taken in the image from which the number of fluorescent particles is counted.

公开(公告)号：US20170016911A1

公开(公告)日：2017-01-19

申请号：US15124557

申请日：2015-03-23

Applicant: Konica Minolta, Inc.

Inventor： Hideki GOUDA ,  Takeshi ISODA ,  Yasuhiro WATANABE ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE

IPC: G01N33/68 ,  G01N15/14 ,  G01N21/64 ,  G01N1/30 ,  G01N33/58

CPC classification number: G01N33/6872 ,  G01N1/30 ,  G01N15/1429 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/582 ,  G01N2001/302 ,  G01N2021/6439 ,  G01N2496/80

Abstract: The present invention provides a method capable of more accurately quantifying a biological material expressed on the cell membrane in pathological samples. The present invention is directed to a method for quantifying a biological material (target biological material) expressed on the cell membrane, the method including the steps of: (1a) immunostaining the target biological material with a fluorescent material; (1b) immunostaining another biological material (reference biological material) on the cell membrane with another fluorescent material; (2) using immunostaining images for the target and reference biological materials to identify the fluorescence signal corresponding to the target biological material and to measure the fluorescence signals corresponding to the target and reference biological materials; and (3) correcting the measured value of the fluorescence signal corresponding to the target biological material by a given method to quantify the expression level.

Abstract translation: 本发明提供能够更准确地定量病理样品中在细胞膜上表达的生物材料的方法。 本发明涉及一种量化在细胞膜上表达的生物材料（靶生物材料）的方法，所述方法包括以下步骤：（1a）用荧光材料免疫染色目标生物材料; （1b）用另一种荧光材料在细胞膜上免疫染色另一种生物材料（参考生物材料）; （2）使用靶标和参考生物材料的免疫染色图像来鉴定对应于目标生物材料的荧光信号并测量与靶标和参照生物材料相对应的荧光信号; 和（3）通过给定的方法校正与目标生物材料相对应的荧光信号的测量值以量化表达水平。

公开(公告)号：US20190113497A1

公开(公告)日：2019-04-18

申请号：US16207378

申请日：2018-12-03

Applicant: Konica Minolta, Inc.

Inventor： Yasuhiro WATANABE ,  Shuji ICHITANI ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE

IPC: G01N33/483 ,  G06T7/00 ,  G06T7/11 ,  G06K9/00 ,  G06T5/50 ,  A61B5/00

Abstract: An image processing device (2A) comprises: an input means for inputting a brightfield image representing cell morphology in a tissue section, and a fluorescence image representing, by fluorescent bright spots, the expression of a specific protein in the same range of the tissue section; a first generation means for generating a cell image obtained by extracting a specific site of a cell from the brightfield image; a second generation means for generating an image obtained by extracting bright spot regions from the fluorescence image, creating a brightness profile for each bright spot region, and generating a fluorescent particle image obtained by extracting the fluorescent particles in the bright spot regions on the basis of the fluorescence profile for one fluorescent particle, which serves as a fluorescence bright spot source; and a calculation means for superimposing the cell image and the fluorescent particle image on one another.

公开(公告)号：US20190339251A1

公开(公告)日：2019-11-07

申请号：US16515424

申请日：2019-07-18

Applicant: Konica Minolta, Inc.

Inventor： Yasuhiro WATANABE ,  Shuji ICHITANI ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE

IPC: G01N33/483 ,  G06T5/50 ,  A61B5/00 ,  G06T7/11 ,  G06K9/00 ,  G06T7/00

Abstract: An image processing device (2A) comprises: an input means for inputting a brightfield image representing cell morphology in a tissue section, and a fluorescence image representing, by fluorescent bright spots, the expression of a specific protein in the same range of the tissue section; a first generation means for generating a cell image obtained by extracting a specific site of a cell from the brightfield image; a second generation means for generating an image obtained by extracting bright spot regions from the fluorescence image, creating a brightness profile for each bright spot region, and generating a fluorescent particle image obtained by extracting the fluorescent particles in the bright spot regions on the basis of the fluorescence profile for one fluorescent particle, which serves as a fluorescence bright spot source; and a calculation means for superimposing the cell image and the fluorescent particle image on one another.

公开(公告)号：US20180164277A1

公开(公告)日：2018-06-14

申请号：US15103171

申请日：2014-12-17

Applicant: Konica Minolta, Inc.

Inventor： Yasuhiro WATANABE ,  Shuji ICHITANI ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE

IPC: G01N33/483 ,  A61B5/00 ,  G06T5/50 ,  G06T7/00

CPC classification number: G01N33/4833 ,  A61B5/0071 ,  G06K9/0014 ,  G06T5/50 ,  G06T7/0012 ,  G06T7/11 ,  G06T2207/10064 ,  G06T2207/20221 ,  G06T2207/30024

Abstract: An image processing device (2A) comprises: an input means for inputting a brightfield image representing cell morphology in a tissue section, and a fluorescence image representing, by fluorescent bright spots, the expression of a specific protein in the same range of the tissue section; a first generation means for generating a cell image obtained by extracting a specific site of a cell from the brightfield image; a second generation means for generating an image obtained by extracting bright spot regions from the fluorescence image, creating a brightness profile for each bright spot region, and generating a fluorescent particle image obtained by extracting the fluorescent particles in the bright spot regions on the basis of the fluorescence profile for one fluorescent particle, which serves as a fluorescence bright spot source; and a calculation means for superimposing the cell image and the fluorescent particle image on one another.

公开(公告)号：US20180156699A1

公开(公告)日：2018-06-07

申请号：US15736171

申请日：2016-06-01

Applicant: KONICA MINOLTA, INC.

Inventor： Yasuhiro WATANABE

IPC: G01N1/30 ,  G01N21/64 ,  G01N33/58 ,  G01N33/533

CPC classification number: G01N1/30 ,  G01N1/28 ,  G01N21/64 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/53 ,  G01N33/533 ,  G01N33/587 ,  G01N2001/302 ,  G01N2021/6439 ,  G01N2458/00

Abstract: A signal of fluorescence emitted from a fluorescent particle of a pathological specimen can be increased in sensitivity and be stabilized, thereby resulting in an enhancement in retrieval accuracy of information from a fluorescence image. A pathological specimen including a tissue section subjected to a treatment (immunostaining/FISH staining treatment) for fluorescence-labeling of an objective biomaterial with a fluorescent particle observable in a dark field, based on an immunostaining or FISH method; a packed layer with which the tissue section is covered; and a protection layer with which the packed layer is covered; wherein the refractive indexes of the fluorescent particle, the packed layer and the protection layer (measurement wavelength: 589 nm; measurement temperature: 20° C.; in all) satisfy the conditions of Expressions (1) and (2): |n1−n2|≤0.20  Expression (1) |n2−n3|≤0.15  Expression (2) n1: refractive index (fluorescent particle) n2: refractive index (packed layer) n3: refractive index (protection layer).

公开(公告)号：US20160209548A1

公开(公告)日：2016-07-21

申请号：US15085620

申请日：2016-03-30

Applicant: Konica Minolta, Inc.

Inventor： Yasuhiro WATANABE ,  Kentaro YANO

IPC: G02B1/04 ,  G02F1/1335

CPC classification number: G02B1/04 ,  B29C55/023 ,  B29C55/06 ,  B29C55/08 ,  B29D11/00644 ,  B29K2995/0034 ,  G02B1/105 ,  G02B1/14 ,  G02B5/3033 ,  G02F1/133528 ,  Y10T428/1041 ,  Y10T428/24975

Abstract: The method for manufacturing a polarizing plate entails applying a hydrophilic polymer coating solution onto a thermoplastic resin layer to form a hydrophilic polymer layer; drying the hydrophilic polymer layer on the thermoplastic resin layer to form a laminate; stretching the laminate of the thermoplastic resin layer and the hydrophilic polymer layer in a longitudinal or lateral direction; dyeing the laminate with a dichroic substance to adsorb the dichroic substance onto the hydrophilic polymer layer of the laminate; bonding the dyed, stretched laminate to a substrate having a hard coat layer formed by an application process to form a bonded laminate substrate; and removing the thermoplastic resin layer from the bonded laminate substrate to form a polarizing plate.

Abstract translation: 偏光板的制造方法需要在热塑性树脂层上涂布亲水性聚合物涂布液，形成亲水性聚合物层; 干燥热塑性树脂层上的亲水性聚合物层以形成层压体; 在纵向或横向上拉伸热塑性树脂层和亲水性聚合物层的层压体; 用二色性物质对层压体进行染色以将二色性物质吸附到层压体的亲水性聚合物层上; 将经染色的拉伸层压体粘合到具有通过涂布工艺形成的硬涂层的基材上以形成粘合的层压基板; 从接合层叠基板上除去热塑性树脂层，形成偏光板。

公开(公告)号：US20160371834A1

公开(公告)日：2016-12-22

申请号：US14902410

申请日：2014-06-27

Applicant: KONICA MINOLTA, INC.

Inventor： Yasuhiro WATANABE ,  Takeshi ISODA ,  Kohsuke GONDA ,  Noriaki OHUCHI ,  Mika WATANABE

IPC: G06T7/00 ,  A61B5/00 ,  G06K9/62 ,  G06K9/46 ,  G06T7/60 ,  G06K9/52

CPC classification number: G06T7/0012 ,  A61B5/0062 ,  A61B5/0071 ,  A61B5/7264 ,  A61B2576/00 ,  G01N21/6456 ,  G06K9/00134 ,  G06K9/0014 ,  G06K9/00147 ,  G06K9/4661 ,  G06K9/52 ,  G06K9/6215 ,  G06T3/0068 ,  G06T7/33 ,  G06T7/60 ,  G06T2207/10016 ,  G06T2207/10024 ,  G06T2207/10061 ,  G06T2207/10064 ,  G06T2207/20036 ,  G06T2207/20221 ,  G06T2207/30004 ,  G06T2207/30024

Abstract: An image processing device includes an input unit and an alignment unit. The input unit inputs a cell shape image and a fluorescence image. The cell shape image shows a shape of a cell in a tissue section. The fluorescence image shows expression of a specific protein as a fluorescent bright point in a region same as a region in the tissue section. The alignment unit aligns the cell shape image and the fluorescence image based on an information source detected in both the cell shape image and the fluorescence image.

Abstract translation: 图像处理装置包括输入单元和对准单元。 输入单元输入单元格形状图像和荧光图像。 细胞形状图像显示组织切片中的细胞的形状。 荧光图像显示与组织切片中的区域相同的区域中的特定蛋白质作为荧光亮点的表达。 对准单元基于在细胞形状图像和荧光图像两者中检测到的信息源来对齐细胞形状图像和荧光图像。

公开(公告)号：US20160187553A1

公开(公告)日：2016-06-30

申请号：US15059981

申请日：2016-03-03

Applicant: Konica Minolta, Inc.

Inventor： Hideyuki SATO ,  Takahiro TAKAGI ,  Yasuhiro WATANABE

IPC: G02B5/30 ,  G02B1/14 ,  G02F1/1335

CPC classification number: G02B5/3033 ,  B29D11/00644 ,  B29D11/0073 ,  B32B2457/202 ,  C08B3/06 ,  C09D101/12 ,  G02B1/14 ,  G02F1/133528 ,  Y10T156/10 ,  Y10T428/1036 ,  Y10T428/1041 ,  Y10T428/105

Abstract: Disclosed is a method of manufacturing a polarizing plate safe in operation, less burdensome on the environment, and with excellent adhesion to a polarizer; also disclosed are a polarizing plate manufactured using said method, and a liquid crystal display device using said polarizing plate. In the method of manufacturing a polarizing plate, a polarizing plate is manufactured in which a protective film which is hydrophilized by alkali saponification is laminated to at least one surface of the polarizer. In the method, said protective film contains cellulose acetate, and the surface free energy before alkali saponification of said protective film satisfies formula (SI), below, and the surface free energy after alkali saponification satisfies formula (SII), below. Formula (SI): 0.25≦γsh/γsp≦0.40; Formula (SII): 1.5≦γsh/γsp≦3.0 (wherein γsh represents the hydrogen bond component of the surface free energy, and γsp represents the dipole component).

Abstract translation: 公开了一种制造在操作中安全的偏振片的方法，对环境的负担更小，并且对偏振器具有优异的粘合性; 还公开了使用所述方法制造的偏振片和使用所述偏振片的液晶显示装置。 在制造偏光板的方法中，制造了通过碱皂化而亲水化的保护膜层压在偏振片的至少一个表面上的偏光板。 在该方法中，所述保护膜含有乙酸纤维素，所述保护膜的碱皂化前的表面自由能满足下式（SI），碱皂化后的表面自由能满足下式（SII）。 式（SI）：0.25＆nlE;γsh/γsp＆nlE; 0.40; 式（SII）：1.5＆nlE;γsh/γsp＆nlE; 3.0（其中γsh表示表面自由能的氢键成分，γsp表示偶极子成分）。