公开(公告)号：US20230271179A1

公开(公告)日：2023-08-31

申请号：US17992084

申请日：2022-11-22

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Tae Song KIM ,  Dong Hyun KANG

IPC: B01L3/00 ,  C08L53/00 ,  C08J3/075

CPC classification number: B01L3/502707 ,  C08J3/075 ,  C08L53/00 ,  B01L2300/0829 ,  B01L2300/12 ,  B01L2300/16 ,  C08J2353/00

Abstract: The present invention relates to a method for manufacturing a three-dimensional structure based on a block copolymer. The method comprises the steps of injecting a block copolymer (BCP) solution into each micro-well formed on the substrate and drying it to form a block copolymer layer, and applying a buffer to the block copolymer layer to hydrate the micro-well in three dimensions Forming the structure, after the three-dimensional structure is formed, injecting and curing a hydrogel solution around the three-dimensional structure may include the step of enhancing stability. In particular, the process of hydration by applying a buffer to the micro-well is performed while an electric field is applied. By controlling the concentration of the block copolymer (BCP) and the amplitude and frequency of the electric field, a three-dimensional artificial cell membrane having a desired size and shape, such as a spherical or ciliary shape and high stability (100% survival for 50 days) is manufactured can do. The present invention can be efficiently applied to various biological fields such as artificial cells, cell-mimicking biosensors, and bioreactors.

公开(公告)号：US20240116005A1

公开(公告)日：2024-04-11

申请号：US18478904

申请日：2023-09-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Tae Song KIM ,  Dong Hyun KANG ,  Bong Kyu KIM

IPC: B01D69/14 ,  C25D1/00 ,  C25D1/02 ,  G01N33/487

CPC classification number: B01D69/144 ,  C25D1/003 ,  C25D1/02 ,  G01N33/487

Abstract: The present invention relates to a method for preparing a 3D biological bilayer membrane structure in a physiological buffer solution and a 3D biological bilayer membrane structure using the same, and more particularly, to a method for preparing a 3D biological bilayer membrane structure that is tightly sealed even under physiological ionic conditions by applying pressure during electroformation to improve a membrane fusion function, and a 3D biological bilayer membrane structure using the same.