公开(公告)号：US20200340001A1

公开(公告)日：2020-10-29

申请号：US16857089

申请日：2020-04-23

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sung Su LIM ,  Seul Gi SHIN ,  Yun Kyung KIM

IPC: C12N15/64 ,  C07K14/47

Abstract: The present disclosure relates to a vector pair for screening TDP-43 (TAR DNA-binding protein 43 or TransActive Response DNA-binding protein 43) oligomer formation, a cell line transfected with the vector pair, and a method of monitoring TDP-43 oligomer formation using the cell line. More specifically, the vector pair includes: a first vector including a first TDP-43 gene and a first fluorescence protein gene; and a second vector including a second TDP-43 gene and a second fluorescence protein gene, wherein a protein expressed from the first fluorescence protein gene and a protein expressed from the second fluorescence protein gene bind to each other to display fluorescence, by association between a protein expressed from the first TDP-43 gene and a protein expressed from the second TDP-43 gene. The vector pair is effective in that it makes it possible to monitor TDP-43 oligomer formation in living cells.

公开(公告)号：US20210255203A1

公开(公告)日：2021-08-19

申请号：US17021438

申请日：2020-09-15

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Ghil Soo NAM ,  Hyun Ah CHOO ,  Ahmed A. Elbatrawy ,  Gyo Chang KEUM ,  Yun Kyung KIM ,  Sung Su LIM ,  Jae Kyun LEE ,  Eun Kyoung BANG

IPC: G01N33/68 ,  C07D215/20 ,  C07D401/06

Abstract: Disclosed are a compound with near-infrared fluorescence that selectively binds to tau aggregates, a method for preparing the same, a tau-targeting near-infrared fluorescent probe including the compound, a composition for detecting a tau fiber protein containing the near-infrared fluorescent probe as an active ingredient, and the use of the composition for the diagnosis of tauopathy. In particular, the compound does not bind to an amyloid beta protein and has high selectivity to a tau aggregate, specifically reported as an etiology of the initial state of tauopathy, thus being useful as a near-infrared fluorescent detector for detecting a tau fiber protein for early diagnosis of a tauopathy including Alzheimer's disease.