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公开(公告)号：US11459546B2
公开(公告)日：2022-10-04
申请号：US16336985
申请日：2017-09-27
Inventor： Yuta Murakami , Yasuhiro Yoshioka , Ping Dai , Toshikazu Yoshikawa , Yoshinori Harada
IPC: C12N5/077 , C12N5/0793
Abstract: The present invention addresses the problem of providing: a method for producing brown adipocytes, osteoblasts, cartilage cells, neural cells, or cardiac cells from somatic cells without performing artificial gene transfer; brown adipocytes, osteoblasts, cartilage cells, neural cells, or cardiac cells; or a composition including a combination of chemical substances that can be used for the aforementioned production method. An example of the present invention is a method for producing brown adipocytes, osteoblasts, cartilage cells, neural cells, or cardiac cells including a step for culturing somatic cells in the presence or absence of an inhibitor or activator selected from the group consisting of an ALK5 inhibitor, an ALK6 inhibitor, an AMPK inhibitor, a cAMP activator, an ALK2 inhibitor, an ALK3 inhibitor, a GSK3 inhibitor, and an Erk inhibitor.
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Patent Agency Ranking
公开(公告)号：US20230082555A1
公开(公告)日：2023-03-16
申请号：US17779639
申请日：2020-11-18
Inventor： Ping Dai , Yukimasa Takeda , Yoshinori Harada , Junichi Matsumoto
IPC: C12N5/077
Abstract: The present invention is primarily directed to provide a new composition for a medium which can be used for differentiation induction from somatic cells to brown adipocytes.
The present invention can include, for example, a composition for a medium used in differentiation induction from somatic cells to brown adipocytes, wherein the composition comprises the following seven components: a thyroid hormone receptor agonist, a glucocorticoid receptor agonist, a phosphodiesterase inhibitor, insulin, an ascorbic acid derivative, albumin, and an antibiotic.
According to the present invention, direct differentiation induction from somatic cells to brown adipocytes can be effectively performed. In addition, according to the present invention, it is possible to effectively maintain brown adipocytes.-
公开(公告)号：US20230059404A1
公开(公告)日：2023-02-23
申请号：US17779567
申请日：2020-11-18
Inventor： Ping Dai , Yukimasa Takeda , Yoshinori Harada , Junichi Matsumoto
Abstract: The present invention is primarily directed to provide a new process capable of performing direct conversion of or induction from a somatic cell to brown adipocytes with low molecular weight compounds, without performing artificial gene transfer.
The present invention includes, for example, a process for producing brown adipocytes by direct differentiation induction from somatic cells, comprising a step of culturing a somatic cell in a serum-free differentiation induction medium in the presence of a selective PPARγ agonist and a cAMP inducer.
According to the present invention, direct conversion of or induction from somatic cells to brown adipocytes can be effectively performed without gene transfer. The brown adipocytes obtained by the present invention are useful as regenerative medicine, models of human brown adipocytes and human beige cells, and the like.-
公开(公告)号：US11781108B2
公开(公告)日：2023-10-10
申请号：US15544604
申请日：2016-01-18
Inventor： Ping Dai , Tetsuro Takamatsu
IPC: C12N5/0793 , A61K35/30 , A61K35/33
CPC classification number: C12N5/0619 , A61K35/30 , A61K35/33 , C12N2501/01 , C12N2501/15 , C12N2501/155 , C12N2501/405 , C12N2501/727 , C12N2506/1307
Abstract: The present invention provides a method with which it is possible to directly induce nervous system cells efficiently and in a short amount of time. Because the method is easy to scale up and is not affected by the characteristics or background of the somatic cells used as material, the method enables the stable supply of nervous system cells. The nervous system cells obtained by the method are useful in various fields of research and healthcare.
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公开(公告)号：US11834679B2
公开(公告)日：2023-12-05
申请号：US16960441
申请日：2019-01-24
Inventor： Ping Dai , Yukimasa Takeda , Yoshinori Harada , Junichi Matsumoto , Ayumi Kusaka
IPC: C12N5/077
CPC classification number: C12N5/0657 , C12N2506/1307
Abstract: The present invention chiefly aims to provide a process for directly inducing cardiomyocytes from somatic cells without performing artificial gene transfer, a cardiomyocyte obtained thereby, and a composition comprising a combination of chemical compounds capable of using for the said process. The present invention can include a process for producing a cardiomyocyte by inducing differentiation directly from a somatic cell, the process comprising a step of culturing the somatic cell in the presence of a MEK inhibitor and a cAMP inducer, and a cardiomyocyte obtained thereby, and then a composition for producing a cardiomyocyte by inducing differentiation directly from a somatic cell, the composition comprising a MEK inhibitor and a cAMP inducer. The cardiomyocytes obtained according to the present invention are useful in regenerative medicine and the like.
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公开(公告)号：US12018283B2
公开(公告)日：2024-06-25
申请号：US16962190
申请日：2019-01-24
Inventor： Ping Dai , Yukimasa Takeda , Yoshinori Harada , Junichi Matsumoto , Ayumi Kusaka
IPC: C12N5/071
CPC classification number: C12N5/0676 , C12N2501/999 , C12N2506/1307
Abstract: The present invention chiefly aims to provide a process for directly inducing insulin-producing cells from somatic cells without performing artificial gene transfer. The present invention can include a process for producing an insulin-producing cell by inducing differentiation directly from a somatic cell, the process comprising a step of culturing the somatic cell in the presence of a cAMP inducer, and six members selected from the group consisting of a GSK3 inhibitor, a TGF-β inhibitor, a BMP inhibitor, a p53 inhibitor, a PI3K inhibitor, a Notch inhibitor and a RAR agonist, or all members thereof. The insulin-producing cells obtained by the present invention are useful in regenerative medicine and the like.
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