公开(公告)号：US20160341686A1

公开(公告)日：2016-11-24

申请号：US15156648

申请日：2016-05-17

申请人： Kalle LEVON ,  Yanyan Wang

发明人： Kalle LEVON ,  Yanyan Wang

IPC分类号： G01N27/327 ,  C25D15/00 ,  C12Q1/00

CPC分类号： C12Q1/006 ,  B82Y30/00 ,  C12Q1/003 ,  C25D3/48 ,  C25D5/18 ,  C25D5/54 ,  G01N27/3278 ,  G01N2333/904

摘要： Fabrication of a high sensitivity potentiometric biosensor is described. The present inventors have developed and characterized a novel amplification platform using a gold nanoparticle (GNPs) electrodeposition method. The synthesized GNP sizes were found to be dependent of HAuCl4 concentration, media acid, scan cycles and scan rate. A systematic investigation into the adsorption of different sizes of proteins from aqueous electrolyte solution onto the electrodeposited GNPs surface by the potentiometric method was performed. Results suggest that the size of different proteins affect how they bond to different sizes of GNPs. This GNPs-based biosensor can retain the native-like structure of proteins, and successfully detect proteins at a high sensitivity level. The resulting glucose and immune biosensors also exhibit low detection limit and wide linear range. This improvement to potentiometric devices enables them to serve as highly sensitive detectors for biomolecules and provides a model that can be used to predict protein bonding on nanoparticles.

摘要翻译： 描述了高灵敏度电位生物传感器的制造。 本发明人已经开发并表征了一种使用金纳米颗粒（GNP）电沉积方法的新型扩增平台。 发现合成的GNP大小取决于HAuCl4浓度，培养基酸，扫描周期和扫描速率。 进行了通过电位法对电沉积的GNP表面吸附不同大小蛋白质的电解质溶液的系统研究。 结果表明，不同蛋白质的大小影响它们如何结合不同大小的GNP。 这种基于GNP的生物传感器可以保留蛋白质的天然样结构，并成功地检测高灵敏度水平的蛋白质。 所得到的葡萄糖和免疫生物传感器也具有低的检测限和宽的线性范围。 这种对电位器件的改进使其能够用作生物分子的高灵敏度检测器，并提供可用于预测纳米粒子上的蛋白质结合的模型。

公开(公告)号：US20090108831A1

公开(公告)日：2009-04-30

申请号：US12328893

申请日：2008-12-05

申请人： Kalle LEVON ,  Arifur Rahman ,  Tsunehiro Sai ,  Ben Zhao

发明人： Kalle LEVON ,  Arifur Rahman ,  Tsunehiro Sai ,  Ben Zhao

IPC分类号： G01N27/00

CPC分类号： G01N27/4145 ,  G01N27/4148

摘要： Specific ionic interactions with a sensing material that is electrically coupled with the floating gate of a floating gate-based ion sensitive field effect transistor (FGISFET) may be used to sense a target material. For example, an FGISFET can use (e.g., previously demonstrated) ionic interaction-based sensing techniques with the floating gate of floating gate field effect transistors. The floating gate can serves as a probe and an interface to convert chemical and/or biological signals to electrical signals, which can be measured by monitoring the change in the device's threshold voltage, VT.

摘要翻译： 可以使用与浮置栅极离子敏感场效应晶体管（FGISFET）的浮动栅极电耦合的感测材料的特定离子相互作用来感测目标材料。 例如，FGISFET可以使用浮动栅极场效应晶体管的浮动栅极（例如，先前证明的）基于离子相互作用的感测技术。 浮动栅极可以用作探测器和将化学和/或生物信号转换为电信号的接口，这可以通过监测器件的阈值电压VT的变化来测量。

公开(公告)号：US20090061464A1

公开(公告)日：2009-03-05

申请号：US12176942

申请日：2008-07-21

申请人： Kalle LEVON ,  Bin Yu ,  Yanxiu Zhou

发明人： Kalle LEVON ,  Bin Yu ,  Yanxiu Zhou

IPC分类号： G01N33/554

CPC分类号： C12Q1/00 ,  C07K9/00 ,  C07K14/32 ,  C07K17/00 ,  C12Q1/003 ,  G01N33/53 ,  G01N33/54353 ,  G01N33/5438 ,  G01N33/54393 ,  G01N33/552

摘要： A real-time method employing a portable peptide-containing potentiometric biosensor, can directly detect and/or quantify bacterial spores. Two peptides for specific recognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example, B. subtilis spores in a concentration range of 0.08-7.3×104 CFU/ml. The sensing method exhibited highly selective recognition properties towards Bacillus subtilis spores over other kinds of spores. The selectivity coefficients of the sensors for other kinds of spores are in the range of 0-1.0×10−5. The biosensor method not only has the specificity to distinguish Bacillus subtilis spores in a mixture of B. subtilis and B. thuringiensis (thur.) Kurstaki spores, but also can discriminate between live and dead B. subtilis spores. Furthermore, the sensing method can distinguish a Bacillus subtilis 1A700 from other B. subtilis strain. Assay time may be as low as about 5 minutes for a single test. Rapid identification of B. anthracis Sterne and B. anthracis ΔAmes was also provided.

摘要翻译： 采用便携式含肽电位生物传感器的实时方法可以直接检测和/或定量细菌孢子。 用于特异性识别枯草芽孢杆菌和炭疽芽孢杆菌的两种肽可以通过聚硅氧烷单层固定（PMI）技术来固定。 传感器通过检测例如浓度范围为0.08-7.3×10 4 CFU / ml的枯草芽孢杆菌孢子将生物识别事件转化为潜在的变化。 该感测方法在其他种类的孢子上表现出对枯草芽孢杆菌孢子的高选择性识别性能。 其他类型孢子的传感器的选择性系数在0-1.0x10-5的范围内。 生物传感器方法不仅具有区分枯草芽孢杆菌和苏云金芽孢杆菌（Thur。）Kurstaki孢子的混合物中的枯草芽孢杆菌孢子的特异性，而且可以区分活枯枯病芽孢杆菌孢子和死枯病芽孢杆菌孢子。 此外，感测方法可以将枯草芽孢杆菌1A700与其他枯草芽孢杆菌菌株区分开。 单次测试的测定时间可能低至约5分钟。 炭疽杆菌和炭疽杆菌的快速鉴定也提供了DeltaAmes。

公开(公告)号：US20090318788A1

公开(公告)日：2009-12-24

申请号：US12109836

申请日：2008-04-25

申请人： Kalle LEVON ,  Miriam RAFAILOVICH ,  Basil RIGAS ,  Yantian Wang

发明人： Kalle LEVON ,  Miriam RAFAILOVICH ,  Basil RIGAS ,  Yantian Wang

IPC分类号： A61B5/1468

CPC分类号： G01N33/5438 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/003 ,  G01N2610/00

摘要： The present invention relates to combining surface molecular imprinting (SMI) with the production of self-assembled monolayers (SAM) of hydroxyl alkanethiolate molecules on gold coated chip surfaces. In this technique, the sensing element is placed on the transducer and the whole assembly can then be miniaturized and integrated into a smart chip. These sensors can detect, nanomolar quantities of complex biomolecules.

摘要翻译： 本发明涉及将表面分子印迹（SMI）与在金涂覆的芯片表面上生产羟基链烷硫醇盐分子的自组装单层（SAM）。 在这种技术中，感测元件放置在换能器上，然后可以将整个组件小型化并集成到智能芯片中。 这些传感器可以检测纳摩尔量的复合生物分子。