Gene recombinant with biodegradability for chlorinated ethylene and
bio-treatment of chlorinated ethylene therewith
    1.
    发明授权
    Gene recombinant with biodegradability for chlorinated ethylene and bio-treatment of chlorinated ethylene therewith 失效
    用氯化乙烯生物降解的基因重组和氯化乙烯的生物处理

    公开(公告)号:US6093556A

    公开(公告)日:2000-07-25

    申请号:US106638

    申请日:1998-06-29

    CPC分类号: C12N9/0073 B09C1/10 C07K14/22

    摘要: The gene recombinant according to the present invention is capable of expressing a biodegradability for chlorinated ethylene and has a recombinant DNA sequence carried on a chromosome, which sequence comprisesa phenol-hydroxylase gene originating from a phenol-metabolizable bacterium capable of bio-degrading chlorinated ethylene, wherein the phenol-hydroxylase gene comprises a gene for facilitating decomposition of chlorinated ethylene and a series of genes for decomposing chlorinated ethylene, anda promoter inserted upstream from the phenol-hydroxylase gene, and/ora terminator inserted downstream from the phenol-hydroxylase gene.

    摘要翻译: 根据本发明的重组基因能够表达氯代乙烯的生物降解性,并且具有携带在染色体上的重组DNA序列,该序列包含源于能够生物降解氯化乙烯的酚代谢细菌的酚羟化酶基因 其中所述酚羟化酶基因包含促进氯化乙烯分解的基因和用于分解氯化乙烯的一系列基因,以及插入酚羟化酶基因上游的启动子和/或插入酚羟化酶下游的终止子 基因。

    Solid-liquid separating method, solid-liquid separator and solidified product
    2.
    发明授权
    Solid-liquid separating method, solid-liquid separator and solidified product 有权
    固液分离方法,固液分离器和固化产物

    公开(公告)号:US08038916B2

    公开(公告)日:2011-10-18

    申请号:US11493098

    申请日:2006-07-26

    申请人: Kanji Nakamura

    发明人: Kanji Nakamura

    IPC分类号: B01D29/00

    摘要: To provide a reliable solid-liquid separating method and apparatus for a slurry, even though the solid component of the slurry is finely divided particles and/or of a shape difficult to entangle, without exudation through gaps in processed portions and, also, with no need to exercise a maintenance work such as replacement of filters, a solid-liquid separating apparatus includes a mold having a cylindrical inner peripheral surface, a gate for opening and closing a second open end of the mold, and a pressing rod slidable into the mold to compress the slurry. Sheet-like fibrous filters are placed on a leading end face of the pressing rod and an inner surface of the gate to seal gaps between the mold and the pressing rod and between the gate and mold. With the filters so placed, the slurry within the mold unit is compressed to provide a solidified product, thereby accomplishing solid-liquid separation.

    摘要翻译: 为了提供可靠的用于浆料的固液分离方法和装置,即使浆料的固体组分是细分散的颗粒和/或形状难以缠结,而不通过加工部分中的间隙渗出,也没有 需要进行诸如更换过滤器的维护工作,固液分离装置包括具有圆筒形内周面的模具,用于打开和关闭模具的第二开口端的浇口以及可滑入模具中的按压杆 压浆。 片状纤维过滤器放置在压杆的前端面和浇口的内表面上,以密封模具和压杆之间以及浇口和模具之间的间隙。 在过滤器如此放置的情况下,模具单元内的浆料被压缩以提供固化产物,从而实现固液分离。

    Solid-liquid separating method,solid-liquid separator and solidified product
    3.
    发明申请
    Solid-liquid separating method,solid-liquid separator and solidified product 有权
    固液分离方法,固液分离器和固化产物

    公开(公告)号:US20070023949A1

    公开(公告)日:2007-02-01

    申请号:US11493098

    申请日:2006-07-26

    申请人: Kanji Nakamura

    发明人: Kanji Nakamura

    IPC分类号: B27N3/00 B29C43/32

    摘要: To provide a reliable solid-liquid separating method and apparatus for a slurry, even though the solid component of the slurry is finely divided particles and/or of a shape difficult to entangle, without exudation through gaps in processed portions and, also, with no need to exercise a maintenance work such as replacement of filters, a solid-liquid separating apparatus includes a mold having a cylindrical inner peripheral surface, a gate for opening and closing a second open end of the mold, and a pressing rod slidable into the mold to compress the slurry. Sheet-like fibrous filters are placed on a leading end face of the pressing rod and an inner surface of the gate to seal gaps between the mold and the pressing rod and between the gate and mold. With the filters so placed, the slurry within the mold unit is compressed to provide a solidified product, thereby accomplishing solid-liquid separation.

    摘要翻译: 为了提供可靠的用于浆料的固液分离方法和装置,即使浆料的固体组分是细分散的颗粒和/或形状难以缠结,而不通过加工部分中的间隙渗出,也没有 需要进行诸如更换过滤器的维护工作,固液分离装置包括具有圆筒形内周面的模具,用于打开和关闭模具的第二开口端的浇口以及可滑入模具中的按压杆 压浆。 片状纤维过滤器放置在压杆的前端面和浇口的内表面上,以密封模具和压杆之间以及浇口和模具之间的间隙。 在过滤器如此放置的情况下,模具单元内的浆料被压缩以提供固化产物,从而实现固液分离。

    SOLID-LIQUID SEPARATING METHOD, SOLID-LIQUID SEPARATOR AND SOLIDIFIED PRODUCT
    4.
    发明申请
    SOLID-LIQUID SEPARATING METHOD, SOLID-LIQUID SEPARATOR AND SOLIDIFIED PRODUCT 有权
    固液分离方法,固液分离器和固化产品

    公开(公告)号:US20120018361A1

    公开(公告)日:2012-01-26

    申请号:US13230874

    申请日:2011-09-13

    申请人: Kanji Nakamura

    发明人: Kanji Nakamura

    IPC分类号: B01D33/01 B01D35/28

    摘要: To provide a reliable solid-liquid separating method and apparatus for a slurry, even though the solid component of the slurry is finely divided particles and/or of a shape difficult to entangle, without exudation through gaps in processed portions and, also, with no need to exercise a maintenance work such as replacement of filters, a solid-liquid separating apparatus includes a mold having a cylindrical inner peripheral surface, a gate for opening and closing a second open end of the mold, and a pressing rod slidable into the mold to compress the slurry. Sheet-like fibrous filters are placed on a leading end face of the pressing rod and an inner surface of the gate to seal gaps between the mold and the pressing rod and between the gate and mold. With the filters so placed, the slurry within the mold unit is compressed to provide a solidified product, thereby accomplishing solid-liquid separation.

    摘要翻译: 为了提供可靠的用于浆料的固液分离方法和装置,即使浆料的固体组分是细碎的颗粒和/或形状难以缠结,而不会通过加工部分中的间隙渗出,也没有 需要进行诸如更换过滤器的维护工作,固液分离装置包括具有圆筒形内周面的模具,用于打开和关闭模具的第二开口端的浇口以及可滑入模具中的按压杆 压浆。 片状纤维过滤器放置在压杆的前端面和浇口的内表面上,以密封模具和压杆之间以及浇口和模具之间的间隙。 在过滤器如此放置的情况下,模具单元内的浆料被压缩以提供固化产物,从而实现固液分离。

    Nucleic acid, nucleic acid for detecting chlorinated ethylene-decomposing bacteria, probe, method of detecting chlorinated ethylene-decomposing bacteria, and method of decomposing chlorinated ethylene
    5.
    发明申请
    Nucleic acid, nucleic acid for detecting chlorinated ethylene-decomposing bacteria, probe, method of detecting chlorinated ethylene-decomposing bacteria, and method of decomposing chlorinated ethylene 审中-公开
    核酸,用于检测氯化乙烯分解细菌的核酸,探针,检测氯化乙烯分解菌的方法和分解氯化乙烯的方法

    公开(公告)号:US20070059749A1

    公开(公告)日:2007-03-15

    申请号:US11517144

    申请日:2006-09-06

    摘要: Chlorinated ethylene-decomposition bacteria is detected by performing PCR using nucleic acid comprising 18˜25 nucleotides that preferentially hybridizes to the 16S rRNA or rDNA of chlorinated ethylene-decomposing bacteria and has any of base sequences of SEQ ID No. 1˜15, a base sequence that has at least 90% homology with any of these base sequences, or a base sequence complementary to any of these base sequences as the primer and the nucleic acid in a sample as the template. The DNA fragment that has been synthesized is detected. Chlorinated ethylene or ethane is decomposed by introducing the chlorinated ethylene-decomposing bacteria detected by this method to contaminated soil or underground water.

    摘要翻译: 通过使用优先与氯化乙烯分解细菌的16S rRNA或rDNA杂交的18〜25个核苷酸的核酸进行PCR来检测氯化乙烯分解菌,并且具有SEQ ID No.1〜15的碱基序列,碱基序列 与这些碱基序列中的任一个具有至少90%的同源性的序列,或与作为模板的样品中的引物和核酸的任何这些碱基序列互补的碱基序列。 检测已经合成的DNA片段。 氯化乙烯或乙烷通过将通过该方法检测的氯化乙烯分解菌引入污染的土壤或地下水来分解。

    Nucleic acid, nucleic acid for detecting chlorinated ethylene-decomposing bacteria, probe, method of detecting chlorinated ethylene-decomposing bacteria, and method of decomposing chlorinated ethylene or ethane
    6.
    发明申请
    Nucleic acid, nucleic acid for detecting chlorinated ethylene-decomposing bacteria, probe, method of detecting chlorinated ethylene-decomposing bacteria, and method of decomposing chlorinated ethylene or ethane 审中-公开
    核酸,用于检测氯化乙烯分解细菌的核酸,探针,检测氯化乙烯分解细菌的方法,以及分解氯化乙烯或乙烷的方法

    公开(公告)号:US20050136410A1

    公开(公告)日:2005-06-23

    申请号:US10741658

    申请日:2003-12-19

    摘要: Chlorinated ethylene-decomposition bacteria is detected by performing PCR using nucleic acid comprising 18˜25 nucleotides that preferentially hybridizes to the 16S rRNA or rDNA of chlorinated ethylene-decomposing bacteria and has any of base sequences of SEQ ID No. 1˜15, a base sequence that has at least 90% homology with any of these base sequences, or a base sequence complementary to any of these base sequences as the primer and the nucleic acid in a sample as the template. The DNA fragment that has been synthesized is detected. Chlorinated ethylene or ethane is decomposed by introducing the chlorinated ethylene-decomposing bacteria detected by this method to contaminated soil or underground water.

    摘要翻译: 通过使用优先与氯化乙烯分解细菌的16S rRNA或rDNA杂交的18〜25个核苷酸的核酸进行PCR来检测氯化乙烯分解菌,并且具有SEQ ID No.1〜15的碱基序列,碱基序列 与这些碱基序列中的任一个具有至少90%的同源性的序列,或与作为模板的样品中的引物和核酸的任何这些碱基序列互补的碱基序列。 检测已经合成的DNA片段。 氯化乙烯或乙烷通过将通过该方法检测的氯化乙烯分解菌引入污染的土壤或地下水来分解。

    Solid-liquid separating method, solid-liquid separator and solidified product
    7.
    发明授权
    Solid-liquid separating method, solid-liquid separator and solidified product 有权
    固液分离方法,固液分离器和固化产物

    公开(公告)号:US08297960B2

    公开(公告)日:2012-10-30

    申请号:US13230874

    申请日:2011-09-13

    申请人: Kanji Nakamura

    发明人: Kanji Nakamura

    IPC分类号: B28B3/04

    摘要: To provide a reliable solid-liquid separating method and apparatus for a slurry, even though the solid component of the slurry is finely divided particles and/or of a shape difficult to entangle, without exudation through gaps in processed portions and, also, with no need to exercise a maintenance work such as replacement of filters, a solid-liquid separating apparatus includes a mold having a cylindrical inner peripheral surface, a gate for opening and closing a second open end of the mold, and a pressing rod slidable into the mold to compress the slurry. Sheet-like fibrous filters are placed on a leading end face of the pressing rod and an inner surface of the gate to seal gaps between the mold and the pressing rod and between the gate and mold. With the filters so placed, the slurry within the mold unit is compressed to provide a solidified product, thereby accomplishing solid-liquid separation.

    摘要翻译: 为了提供可靠的用于浆料的固液分离方法和装置,即使浆料的固体组分是细碎的颗粒和/或形状难以缠结,而不会通过加工部分中的间隙渗出,也没有 需要进行诸如更换过滤器的维护工作,固液分离装置包括具有圆筒形内周面的模具,用于打开和关闭模具的第二开口端的浇口以及可滑入模具中的按压杆 压浆。 片状纤维过滤器放置在压杆的前端面和浇口的内表面上,以密封模具和压杆之间以及浇口和模具之间的间隙。 在过滤器如此放置的情况下,模具单元内的浆料被压缩以提供固化产物,从而实现固液分离。

    NUCLEIC ACID, NUCLEIC ACID FOR DETECTING CHLORINATED ETHYLENE-DECOMPOSING BACTERIA, PROBE, METHOD OF DETECTING CHLORINATED ETHYLENE-DECOMPOSING BACTERIA, AND METHOD OF DECOMPOSING CHLORINATED ETHYLENE
    8.
    发明申请
    NUCLEIC ACID, NUCLEIC ACID FOR DETECTING CHLORINATED ETHYLENE-DECOMPOSING BACTERIA, PROBE, METHOD OF DETECTING CHLORINATED ETHYLENE-DECOMPOSING BACTERIA, AND METHOD OF DECOMPOSING CHLORINATED ETHYLENE 审中-公开
    用于检测氯化乙烯分解细菌的核酸,核酸,探针,检测氯乙烯 - 分解细菌的方法,以及分解氯乙烯的方法

    公开(公告)号:US20080099395A1

    公开(公告)日:2008-05-01

    申请号:US11839187

    申请日:2007-08-15

    IPC分类号: C02F3/30

    摘要: Chlorinated ethylene-decomposition bacteria is detected by performing PCR using nucleic acid comprising 18˜25 nucleotides that preferentially hybridizes to the 16S rRNA or rDNA of chlorinated ethylene-decomposing bacteria and has any of base sequences of SEQ ID No. 1˜15, a base sequence that has at least 90% homology with any of these base sequences, or a base sequence complementary to any of these base sequences as the primer and the nucleic acid in a sample as the template. The DNA fragment that has been synthesized is detected. Chlorinated ethylene or ethane is decomposed by introducing the chlorinated ethylene-decomposing bacteria detected by this method to contaminated soil or underground water.

    摘要翻译: 通过使用优先与氯化乙烯分解细菌的16S rRNA或rDNA杂交的18〜25个核苷酸的核酸进行PCR来检测氯化乙烯分解菌,并且具有SEQ ID No.1〜15的碱基序列,碱基序列 与这些碱基序列中的任一个具有至少90%的同源性的序列,或与作为模板的样品中的引物和核酸的任何这些碱基序列互补的碱基序列。 检测已经合成的DNA片段。 氯化乙烯或乙烷通过将通过该方法检测的氯化乙烯分解菌引入污染的土壤或地下水来分解。

    Recycle of grinding sludge
    9.
    发明授权
    Recycle of grinding sludge 有权
    回收研磨泥浆

    公开(公告)号:US06874411B2

    公开(公告)日:2005-04-05

    申请号:US09838340

    申请日:2001-04-20

    摘要: A method of and an apparatus for compressing the grinding sludge, which is effective to compress the grinding sludge to provide an intended rigid briquette even though the grinding sludge contains ground chips of hardened iron and/or steel materials and an oil-based coolant and also to facilitate reuse of the briquette (B1) as material for production of steel. The grinding sludge containing the oil-based coolant and hardened component parts such as steel component parts that are used in bearings is compressed. To this end, the grinding sludge produced from a grinding line (1) is filtered by a filtering unit (4) to provide a concentrated sludge which is subsequently compressed by squeezing by a briquetting machine (5) to provide the briquette (B1). The briquette B1 so obtained can be reused as steel material. The coolant separated from the grinding sludge during filtering and squeezing is returned to the grinding line (1) through recovery passages (7, 8).

    摘要翻译: 一种用于压缩研磨污泥的方法和装置,其有效地压缩研磨泥浆以提供预期的刚性团块,即使研磨污泥含有硬化铁和/或钢材料的研磨碎片和油基冷却剂,以及 以便于将煤块(B1)作为生产钢材的再利用。 包含油基冷却剂的研磨污泥和用于轴承的钢组分部件等硬化部件被压缩。 为此,由研磨线(1)产生的研磨污泥由过滤单元(4)过滤以提供浓缩的污泥,随后通过压块机(5)的挤压压缩以提供压块(B1)。 如此获得的团块B1可以作为钢材重复使用。 在过滤和挤压期间从研磨污泥分离的冷却剂通过回收通道(7,8)返回到研磨生产线(1)。

    High concentration herbicide formulation
    10.
    发明授权
    High concentration herbicide formulation 失效
    高浓度除草剂配方

    公开(公告)号:US5045109A

    公开(公告)日:1991-09-03

    申请号:US483865

    申请日:1990-02-23

    IPC分类号: A01N25/30 A01N47/12 A01N47/16

    CPC分类号: A01N47/12

    摘要: A high concentration herbicide formulation comprising (1) a herbicidally active thiolcarbamate of the following formula I which is liquid at room temperature, (2) at least one nonionic surfactant of the following formula II and (3) an anionic surfactant of the following formula III: ##STR1## wherein R.sup.1 is an alkyl group having from 1 to 3 carbon atoms, a phenyl group or a halogen-substituted phenyl group, R.sup.2 is an alkyl group having from 1 to 5 carbon atoms, and R.sup.3 is an alkyl group having from 1 to 5 carbon atoms, provided that R.sup.2 and R.sup.3 may form a ring; ##STR2## wherein R.sup.4 is ##STR3## wherein R.sup.5 is a hydrogen atom or a methyl group, m is an integer of from 1 to 3, x is an integer of at least 10, y is an integer of from 0 to 10, provided that x+y is within a range of from 10 to 30, A is an oxyethylene group, and B is an oxypropylene group, provided that the addition form of A and B may be a block-form or a random-form; and ##STR4## wherein R.sup.6 is an alkyl group having from 9 to 15 carbon atoms, M is an alkali metal or an alkaline earth metal, and n is the number of metal ions.

    摘要翻译: 一种高浓度除草剂制剂,其包含(1)在室温下为液体的下式I的除草活性硫代氨基​​甲酸酯,(2)至少一种下式II的非离子表面活性剂和(3)下式III的阴离子表面活性剂 :其中R 1为具有1至3个碳原子的烷基,苯基或卤素取代的苯基,R 2为具有1至5个碳原子的烷基,且R 3为烷基 具有1至5个碳原子的基团,条件是R 2和R 3可以形成环; 其中R 4为,其中R 5为氢原子或甲基,m为1至3的整数,x为至少10的整数,y为0至 10,如果x + y在10〜30的范围内,A是氧化乙烯基,B是氧化丙烯基,条件是A和B的添加形式可以是嵌段形式或无规形式 ; 和(III)其中R6是具有9-15个碳原子的烷基,M是碱金属或碱土金属,n是金属离子的数目。