摘要:
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要:
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要:
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Lepidopteran pest, decreases the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest, thereby limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides as set forth, or active variants and fragments thereof, wherein a decrease in expression of one or more sequences in the target pest has insecticidal activity. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In a specific embodiment, the pest is Spodoptera frugiperda. Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要:
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. Target polynucleotides for specific protein classes and target polynucleotides set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof are provided, wherein a decrease in expression of one or more sequences in the target pest controls the pest. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. Plants, plant part, bacteria and host cells comprising the silencing elements or an active variant or fragment thereof are also provided.
摘要翻译:提供了使用沉积元件的方法和组合物,其在被害虫摄入时能够降低害虫中靶序列的表达。 在具体实施方案中,靶序列的表达降低控制有害生物,因此方法和组合物能够限制对植物的损害。 提供了SEQ ID NOS:1,2,3,4,5,6,7,8,9,10或其活性变体或片段中列出的特异性蛋白质类别和靶多核苷酸的靶多核苷酸,其中表达降低 目标害虫中的一个或多个序列控制害虫。 进一步提供的是沉积元件,当受害虫摄入时,降低靶多肽的水平,从而控制害虫。 还提供了包含沉默元件或其活性变体或片段的植物,植物部分,细菌和宿主细胞。
摘要:
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译:提供了使用沉积元件的方法和组合物,当沉积元件在被害虫摄入时,例如来自蚜科的害虫中,它们能够降低害虫中靶序列的表达。 在具体实施方案中,靶序列的表达降低控制有害生物,因此方法和组合物能够限制对植物的损害。 本发明提供了用于特异性蛋白质类别的靶多核苷酸以及如SEQ ID NO:1,2,3,4,5,6,7,8,9,10或其活性变体或片段所示的靶多核苷酸,其中a 目标害虫中一个或多个序列的表达减少对害虫的控制(即具有杀虫活性)。 进一步提供的是沉积元件,当受害虫摄入时,降低靶多肽的水平,从而控制害虫。 在具体实施方案中,害虫是蚜虫甘氨酸。 还提供了包含本发明的沉默元件或其活性变体或片段的植物,植物部分,细菌和其它宿主细胞。
摘要:
This invention relates to an isolated nucleic acid fragment encoding scorpion toxins that are K-channel agonists. The invention also relates to the construction of a chimeric gene encoding all or a portion of the K-channel agonists, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the K-channel agonists in a transformed host cell.
摘要:
This invention relates to isolated nucleic acids encoding plant defensins. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant defensin, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of plant defensins in a transformed host cell.
摘要:
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, is capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要:
In particular aspects of the invention, nucleic acid molecules encoding novel proteases identified in the midgut of Diabrotica virgfera virgfera (i.e., western corn rootworm) are provided. The nucleotide sequences encode novel cysteine proteases that belong to the cathepsin L-like subfamily of proteases. Variants and fragments of the nucleotide sequences that encode polypeptide sequences that possess proteolytic activity are further disclosed. Methods of using the nucleic acid sequences that encode novel proteases to protect a plant from an insect pest are provided. Particular embodiments also provide vectors, expression cassettes and transformed host cells comprising a protease nucleic acid of the invention.
摘要:
The present invention relates to isolated nucleic acids encoding plant cyclotides. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.