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    COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS
    1.
    发明申请
    COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS 有权
    用于抑制来自LYGUS的目标多核苷酸的组合物和方法

    公开(公告)号：US20090192117A1

    公开(公告)日：2009-07-30

    申请号：US12351379

    申请日：2009-01-09

    申请人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark E. Nelson ,  James K. Presnail ,  Janet A. Rice

    发明人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark E. Nelson ,  James K. Presnail ,  Janet A. Rice

    IPC分类号： A61K31/7088 ,  C12N5/10 ,  A01H5/00

    CPC分类号： C12N15/8286 ,  A01N57/16 ,  C12N15/113 ,  C12N15/8218 ,  C12N2310/141 ,  C12N2310/531 ,  Y02A40/162

    摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

    摘要翻译： 提供了使用沉积元件的方法和组合物，当沉积元件被害虫摄取时，例如来自莱古氏属属的害虫时，它们能够降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供了本文公开的来自特定多肽家族的多种靶多核苷酸，并进一步提供了SEQ ID NO：1-21所示的靶多核苷酸或其活性变体，其中目标害虫中一个或多个序列的表达降低 控制害虫（即具有杀虫活性）。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，有害生物是黄瓜（Lygus Hesperus）。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

    Compositions and methods for the suppression of target polynucleotides from Lygus
    2.
    发明授权
    Compositions and methods for the suppression of target polynucleotides from Lygus 有权
    用于抑制来自Lygus的靶多核苷酸的组合物和方法

    公开(公告)号：US08809625B2

    公开(公告)日：2014-08-19

    申请号：US12351379

    申请日：2009-01-09

    申请人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark E. Nelson ,  James K. Presnail ,  Janet A. Rice

    发明人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark E. Nelson ,  James K. Presnail ,  Janet A. Rice

    IPC分类号： C12N15/82 ,  C12N15/87 ,  A01H1/00

    CPC分类号： C12N15/8286 ,  A01N57/16 ,  C12N15/113 ,  C12N15/8218 ,  C12N2310/141 ,  C12N2310/531 ,  Y02A40/162

    摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, is capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

    摘要翻译： 提供了使用沉积元件的方法和组合物，当沉积元件在被害虫摄取时，例如来自Ly属的害虫时，能够降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供了本文公开的特异性多肽家族的多种靶多核苷酸或其活性变体，其中目标害虫中一个或多个序列的表达降低控制害虫。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

    Compositions and methods for the suppression of target polynucleotides from lepidoptera
    3.
    发明授权
    Compositions and methods for the suppression of target polynucleotides from lepidoptera 有权
    用于抑制鳞翅目的靶多核苷酸的组合物和方法

    公开(公告)号：US08847013B2

    公开(公告)日：2014-09-30

    申请号：US12351267

    申请日：2009-01-09

    申请人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark Nelson ,  James K. Presnail ,  Janet A. Rice

    发明人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark Nelson ,  James K. Presnail ,  Janet A. Rice

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C07H21/04

    CPC分类号： C12N15/8286 ,  A01N65/20 ,  A01N65/44 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/32 ,  Y02A40/162

    摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Lepidopteran pest, decreases the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest, thereby limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides as set forth, or active variants and fragments thereof, wherein a decrease in expression of one or more sequences in the target pest has insecticidal activity. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In a specific embodiment, the pest is Spodoptera frugiperda. Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

    摘要翻译： 提供了使用沉积元件的方法和组合物，当由有害生物（例如鳞翅目害虫）摄入时，降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制害虫，从而限制对植物的损害。 本发明提供了编码来自特定蛋白质家族的多肽的多核苷酸和如所述的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低具有杀虫活性。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，害虫是草地夜蛾（Spodoptera frugiperda）。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

    Compositions and methods for the suppression of target polynucleotides from the family aphididae
    4.
    发明授权
    Compositions and methods for the suppression of target polynucleotides from the family aphididae 有权
    用于抑制来自家蚜科的靶多核苷酸的组合物和方法

    公开(公告)号：US08367895B2

    公开(公告)日：2013-02-05

    申请号：US12351189

    申请日：2009-01-09

    申请人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark Nelson ,  James K. Presnail ,  Janet A. Rice

    发明人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark Nelson ,  James K. Presnail ,  Janet A. Rice

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C07H21/04

    CPC分类号： C12N15/8286 ,  C07K14/43563 ,  Y02A40/162

    摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. Target polynucleotides for specific protein classes and target polynucleotides set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof are provided, wherein a decrease in expression of one or more sequences in the target pest controls the pest. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. Plants, plant part, bacteria and host cells comprising the silencing elements or an active variant or fragment thereof are also provided.

    摘要翻译： 提供了使用沉积元件的方法和组合物，其在被害虫摄入时能够降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 提供了SEQ ID NOS：1,2,3,4,5,6,7,8,9,10或其活性变体或片段中列出的特异性蛋白质类别和靶多核苷酸的靶多核苷酸，其中表达降低 目标害虫中的一个或多个序列控制害虫。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 还提供了包含沉默元件或其活性变体或片段的植物，植物部分，细菌和宿主细胞。

    COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM THE FAMILY APHIDIDAE
    5.
    发明申请
    COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM THE FAMILY APHIDIDAE 有权
    从家禽APHIDIDA中抑制目标多核苷酸的组合物和方法

    公开(公告)号：US20090192116A1

    公开(公告)日：2009-07-30

    申请号：US12351189

    申请日：2009-01-09

    申请人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark Nelson ,  James K. Presnail ,  Janet A. Rice

    发明人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark Nelson ,  James K. Presnail ,  Janet A. Rice

    IPC分类号： A01N43/02 ,  A01H5/00 ,  C12N5/10 ,  A01P7/00

    CPC分类号： C12N15/8286 ,  C07K14/43563 ,  Y02A40/162

    摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

    摘要翻译： 提供了使用沉积元件的方法和组合物，当沉积元件在被害虫摄入时，例如来自蚜科的害虫中，它们能够降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供了用于特异性蛋白质类别的靶多核苷酸以及如SEQ ID NO：1,2,3,4,5,6,7,8,9,10或其活性变体或片段所示的靶多核苷酸，其中a 目标害虫中一个或多个序列的表达减少对害虫的控制（即具有杀虫活性）。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，害虫是蚜虫甘氨酸。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

    COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA
    6.
    发明申请
    COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA 有权
    用于抑制来自LEPIDOPTERA的目标多核苷酸的组合物和方法

    公开(公告)号：US20090265818A1

    公开(公告)日：2009-10-22

    申请号：US12351267

    申请日：2009-01-09

    申请人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark Nelson ,  James K. Presnail ,  Janet A. Rice

    发明人： Rafael Herrmann ,  Michael Lassner ,  Albert L. Lu ,  Mark Nelson ,  James K. Presnail ,  Janet A. Rice

    IPC分类号： A01H5/00 ,  C12N5/04 ,  A01N43/04 ,  A01P7/04

    CPC分类号： C12N15/8286 ,  A01N65/20 ,  A01N65/44 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/32 ,  Y02A40/162

    摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

    摘要翻译： 提供了使用沉积元件的方法和组合物，当沉积元件在被害虫如来自鳞翅目序列的害虫中摄取时，它们能够降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供编码来自特定蛋白质家族的多肽的多核苷酸和SEQ ID NO：1,2,3,4,5,6,7,8,9,10,11,12,13,14中所示的各种靶多核苷酸的靶多核苷酸 ，15，16，17，18，19，20，21，22，23，24，25，26，27，28，29，30，31，32，33，34，35，36，37，38，39 ，40,41,42,43,44,45,46,47,48,49或50个或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即 ，具有杀虫活性）。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，害虫是草地夜蛾（Spodoptera frugiperda）。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

    Plastid transit peptides
    7.
    发明授权
    Plastid transit peptides 失效
    质体转运肽

    公开(公告)号：US07557260B2

    公开(公告)日：2009-07-07

    申请号：US12015342

    申请日：2008-01-16

    申请人： Michael Lassner ,  Jack Q. Wilkinson

    发明人： Michael Lassner ,  Jack Q. Wilkinson

    IPC分类号： C12N15/09 ,  C12N15/82 ,  A01H5/00 ,  C07H21/00

    CPC分类号： C07K14/415 ,  C07K14/00 ,  C12N15/8221

    摘要： The present invention provides novel plastid transit peptides that direct localization of attached moieties (e.g., polypeptides) into plant plastids. The present invention also relates to methods and compositions for localizing polypeptides to plant plastids including, but not limited to, transgenic plant production.

    摘要翻译： 本发明提供了将附着部分（例如，多肽）定位于植物质体中的新型质体转运肽。 本发明还涉及用于将多肽定位于植物质体的方法和组合物，包括但不限于转基因植物生产。

    Plant lysophosphatidic acid acyltransferases
    10.
    发明授权
    Plant lysophosphatidic acid acyltransferases 失效
    植物溶血磷脂酸酰基转移酶

    公开(公告)号：US5968791A

    公开(公告)日：1999-10-19

    申请号：US458109

    申请日：1995-06-01

    申请人： Huw Maelor Davies ,  Deborah Hawkins ,  Janet Nelson ,  Michael Lassner

    发明人： Huw Maelor Davies ,  Deborah Hawkins ,  Janet Nelson ,  Michael Lassner

    IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12N15/82 ,  C12P7/64

    CPC分类号： C12N9/1029 ,  C12N15/8247

    摘要： This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants and for modification of plant triglyceride compositions are described.

    摘要翻译： 本发明涉及植物LPAAT，鉴定此类蛋白质的方法，与此类蛋白质相关的氨基酸和核酸序列，以及获得，制备和/或使用此类植物LPAAT的方法。 净化，特别是植物膜的去除以及远离其它植物蛋白质的大量分离，并且提供了植物LPAAT的使用，包括使用蛋白质作为用于生物技术应用的基因分离的工具。 另外，提供了编码LPAAT蛋白质区域的核酸序列，并描述了这些序列用于从植物中分离LPAAT基因和修饰植物甘油三酯组合物的用途。