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公开(公告)号：US20050037391A1
公开(公告)日：2005-02-17
申请号：US10864874
申请日：2004-06-10
IPC分类号： A21D8/04 , A23K1/165 , A23L1/09 , C07H21/04 , C12N9/24 , C12N9/28 , C12N9/52 , C12N15/74 , C12Q1/68 , G01N33/554 , G01N33/569
CPC分类号： A21D8/042 , A23K20/189 , A23L29/35 , C12N9/2417 , C12Y302/0106
摘要： We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.
摘要翻译： 我们公开了可从母体多肽衍生的PS4变体多肽，该亲本多肽具有非产麦芽糖外切酶活性，该PS4变体多肽包含一个或多个以下取代：G69P，A141P，G223A，A268P，G313P，S399P和G400P，具有 提及如SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列的位置编号。这样的PS4变体多肽可以用作外切淀粉酶，特别是作为非发酵的外消旋酶。 公开了这些PS4变体多肽与Novamyl的组合。
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公开(公告)号：US07858352B2
公开(公告)日：2010-12-28
申请号：US11635511
申请日：2006-12-08
申请人： Karsten Matthias Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Robert Veltman
发明人： Karsten Matthias Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Robert Veltman
CPC分类号： A21D8/042 , A23K20/189 , A23L29/35 , C12N9/2417 , C12Y302/0106
摘要： We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.
摘要翻译： 我们公开了可从母体多肽衍生的PS4变体多肽，该亲本多肽具有非产麦芽糖外切酶活性，该PS4变体多肽包含一个或多个以下取代：G69P，A141P，G223A，A268P，G313P，S399P和G400P，具有 提及如SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列的位置编号。这样的PS4变体多肽可以用作外切淀粉酶，特别是作为非发酵的外消旋酶。 公开了这些PS4变体多肽与Novamyl的组合。
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公开(公告)号：US07166453B2
公开(公告)日：2007-01-23
申请号：US10864874
申请日：2004-06-10
申请人： Karsten Matthias Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Robert Veltman
发明人： Karsten Matthias Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Robert Veltman
CPC分类号： A21D8/042 , A23K20/189 , A23L29/35 , C12N9/2417 , C12Y302/0106
摘要： We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.
摘要翻译： 我们公开了可从母体多肽衍生的PS4变体多肽，该亲本多肽具有非产麦芽糖外切酶活性，该PS4变体多肽包含一个或多个以下取代：G69P，A141P，G223A，A268P，G313P，S399P和G400P，具有 提及如SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列的位置编号。这样的PS4变体多肽可以用作外切淀粉酶，特别是作为非发酵的外消旋酶。 公开了这些PS4变体多肽与Novamyl的组合。
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4.发明申请公开(公告)号：US20080190219A1
公开(公告)日：2008-08-14
申请号：US10590768
申请日：2005-02-25
IPC分类号： G01N1/02
CPC分类号： C12Q1/24 , C12Q1/04 , G01N2001/021
摘要： The present invention relates to a method, a chip, a device, and a system for collection of biological particles. The method makes use of electrostatic attraction of biological particles to charged electrodes and preferably operates with gaseous samples. The method, chip, device, and system are e.g. useful for collecting pathogenic biological particles, such as bacterial spores and vira, from air samples and allow for subsequent analysis of the collected biological particles.
摘要翻译： 本发明涉及用于收集生物颗粒的方法，芯片，装置和系统。 该方法利用生物粒子对带电电极的静电吸引，优选与气态样品一起操作。 方法，芯片，装置和系统是例如。 可用于从空气样品中收集致病生物颗粒，如细菌孢子和病毒，并允许随后分析所收集的生物颗粒。
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5.发明授权Method, chip, device and integrated system for detection biological particles 有权检测生物粒子的方法，芯片，器件和集成系统公开(公告)号：US07892794B2
公开(公告)日：2011-02-22
申请号：US10590632
申请日：2005-02-25
CPC分类号： C12Q1/04 , C12Q1/24 , G01N2001/021
摘要： The present invention relates to a method, a chip, a device, and a system for detection of biological particles. The method of the invention typically comprises collecting the biological particles from a gaseous sample, contacting the biological particles with a first liquid reagent, extracting biological material from the collected biological particles, and analyzing the biological material for the presence of a target nucleic acid sequence.
摘要翻译： 本发明涉及一种检测生物颗粒的方法，芯片，装置和系统。 本发明的方法通常包括从气态样品中收集生物颗粒，使生物颗粒与第一液体试剂接触，从收集的生物颗粒中提取生物材料，并分析生物材料以存在靶核酸序列。
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公开(公告)号：US07985540B2
公开(公告)日：2011-07-26
申请号：US10590630
申请日：2005-02-25
CPC分类号： C12N15/1003 , C12N1/066 , C12N13/00
摘要： The present invention relates to a method, a chip, a device and a system for extraction of biological material form biological cells. The invention involves exposing the biological particles to an alternating electric field in a sample chamber and may also involve subsequent analysis of the biological material after the extraction.
摘要翻译： 本发明涉及用于从生物细胞中提取生物材料的方法，芯片，装置和系统。 本发明涉及将生物颗粒暴露于样品室中的交变电场，并且还可以涉及在提取之后对生物材料的后续分析。
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公开(公告)号：US06518054B1
公开(公告)日：2003-02-11
申请号：US09381982
申请日：1999-12-07
IPC分类号： C12N900
摘要： The present invention provides genes encoding variants of metallo-endopeptidases that have been engineered to be resistant to prolonged boiling while having maintained their enzymatic performance at much lower temperatures. In addition, thermal stability of the metallo-endopeptidases is highly dependent on calcium at concentrations in the mM range. The invention further provides active metallo-endopeptidases variants whose stability depending on calcium concentration can be changed so as to provide metallo-endopeptidases that are calcium dependent or independent. The invention also provides genes that encode boiling-resistant metallo-endopetidases whose stability depending on calcium concentration can be changed. The invention also provides vectors and cells comprising these genes and proteases produced through these genes, vectors and/or cells. In particular variants with the above described properties are provided of thermolysin-like proteases such as produced by Bacillus stearothermophilus (TLP-ste) and Bacillus thermoproteolyticus (thermolysine). Boiling-resistant and calcium independent or dependent metallo-endopeptidases can be applied in several industrial processes, for instance in the preparation of the artificial sweetener aspartame, but also in the leather industry, in breweries and in the production of protein hydrolysates.
摘要翻译： 本发明提供编码金属内肽酶变体的基因，其已被工程化以对延长的煮沸有抵抗力，同时保持其在较低温度下的酶性能。 此外，金属内肽酶的热稳定性高度依赖于在mM范围内的浓度下的钙。 本发明还提供活性金属内肽酶变体，其可以改变其依赖于钙浓度的稳定性，以提供钙依赖性或独立性的金属内肽酶。 本发明还提供编码耐沸腾金属内肽酶的基因，其可以改变其依赖于钙浓度的稳定性。 本发明还提供了通过这些基因，载体和/或细胞产生的包含这些基因和蛋白酶的载体和细胞。 特别是具有上述性质的变体提供了嗜热脂肪芽孢杆菌（Bacillus stearothermophilus）（TLP-ste）和热嗜热芽孢杆菌（Thermolycolyticus）产生的嗜热菌蛋白酶样蛋白酶。 抗沸腾和钙依赖性或依赖性金属内肽酶可以应用于几个工业过程中，例如制备人造甜味剂阿斯巴甜，还可用于皮革工业，酿酒厂和生产蛋白质水解产物。
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公开(公告)号：US20080176220A1
公开(公告)日：2008-07-24
申请号：US10590648
申请日：2005-02-25
CPC分类号： C12Q1/6853 , C12Q1/686 , C12Q2549/119 , C12Q2527/107
摘要： The present invention relates to a method, a kit and a system for sensitive detection of a target nucleic acid molecule. The target nucleic acid molecule may e.g. be specific for a certain microorganism, e.g. a pathogenic microorganism such as B. anthracis. The methods and kits relate to so-called nested PCR and especially improvements, which renders the nested PCR technique better suited for automated analysis.
摘要翻译： 本发明涉及用于敏感检测靶核酸分子的方法，试剂盒和系统。 靶核酸分子可以是例如。 特定于某种微生物，例如 致病微生物如炭疽杆菌。 这些方法和试剂盒涉及所谓的嵌套PCR，特别是改进，这使得巢式PCR技术更适合于自动化分析。
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9.发明授权公开(公告)号：US07932024B2
公开(公告)日：2011-04-26
申请号：US10590768
申请日：2005-02-25
CPC分类号： C12Q1/24 , C12Q1/04 , G01N2001/021
摘要： The present invention relates to a method, a chip, a device, and a system for collection of biological particles. The method makes use of electrostatic attraction of biological particles to charged electrodes and preferably operates with gaseous samples. The method, chip, device, and system are e.g. useful for collecting pathogenic biological particles, such as bacterial spores and vira, from air samples and allow for subsequent analysis of the collected biological particles.
摘要翻译： 本发明涉及用于收集生物颗粒的方法，芯片，装置和系统。 该方法利用生物粒子对带电电极的静电吸引，优选与气态样品一起操作。 方法，芯片，装置和系统是例如。 可用于从空气样品中收集致病生物颗粒，如细菌孢子和病毒，并允许随后分析所收集的生物颗粒。
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10.发明申请Method, Chip, Device and Integrated System for Detection Biological Particles 有权生物颗粒检测方法，芯片，器件和集成系统公开(公告)号：US20080220414A1
公开(公告)日：2008-09-11
申请号：US10590632
申请日：2005-02-25
CPC分类号： C12Q1/04 , C12Q1/24 , G01N2001/021
摘要： The present invention relates to a method, a chip, a device, and a system for detection of biological particles. The method of the invention typically comprises collecting the biological particles from a gaseous sample, contacting the biological particles with a first liquid reagent, extracting biological material from the collected biological particles, and analysing the biological material for the presence of a target nucleic acid sequence.
摘要翻译： 本发明涉及一种检测生物颗粒的方法，芯片，装置和系统。 本发明的方法通常包括从气态样品中收集生物颗粒，使生物颗粒与第一液体试剂接触，从收集的生物颗粒中提取生物材料，并分析生物材料以存在靶核酸序列。
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