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公开(公告)号：US07858352B2
公开(公告)日：2010-12-28
申请号：US11635511
申请日：2006-12-08
申请人： Karsten Matthias Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Robert Veltman
发明人： Karsten Matthias Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Robert Veltman
CPC分类号： A21D8/042 , A23K20/189 , A23L29/35 , C12N9/2417 , C12Y302/0106
摘要： We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.
摘要翻译： 我们公开了可从母体多肽衍生的PS4变体多肽，该亲本多肽具有非产麦芽糖外切酶活性，该PS4变体多肽包含一个或多个以下取代：G69P，A141P，G223A，A268P，G313P，S399P和G400P，具有 提及如SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列的位置编号。这样的PS4变体多肽可以用作外切淀粉酶，特别是作为非发酵的外消旋酶。 公开了这些PS4变体多肽与Novamyl的组合。
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公开(公告)号：US07166453B2
公开(公告)日：2007-01-23
申请号：US10864874
申请日：2004-06-10
申请人： Karsten Matthias Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Robert Veltman
发明人： Karsten Matthias Kragh , Harm Mulder , Steffen Petersen , Helle Fomsgaard , Oene Robert Veltman
CPC分类号： A21D8/042 , A23K20/189 , A23L29/35 , C12N9/2417 , C12Y302/0106
摘要： We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.
摘要翻译： 我们公开了可从母体多肽衍生的PS4变体多肽，该亲本多肽具有非产麦芽糖外切酶活性，该PS4变体多肽包含一个或多个以下取代：G69P，A141P，G223A，A268P，G313P，S399P和G400P，具有 提及如SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列的位置编号。这样的PS4变体多肽可以用作外切淀粉酶，特别是作为非发酵的外消旋酶。 公开了这些PS4变体多肽与Novamyl的组合。
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公开(公告)号：US08802398B2
公开(公告)日：2014-08-12
申请号：US12915853
申请日：2010-10-29
申请人： Jorn Dalgaard Mikkelsen , Karsten Matthias Kragh , Rene Mikkelsen , Patrick Maria Franciscus Derkx , Shukun Yu , Harm Mulder , Igor Nikolaev
发明人： Jorn Dalgaard Mikkelsen , Karsten Matthias Kragh , Rene Mikkelsen , Patrick Maria Franciscus Derkx , Shukun Yu , Harm Mulder , Igor Nikolaev
CPC分类号： C12N11/14 , C12N9/0006 , C12P19/00
摘要： A method of oxidizing a saccharide, by contacting the saccharide with an alcohol dehydrogenase (ADH) enzyme selected from a quinone redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide (NAD+) redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide phosphate (NADP+) redox cofactor-dependent ADH, and any combination thereof is described. An oxidized saccharide obtainable by the method and products, in particular food products and paper products, containing the oxidized saccharide, are also described.
摘要翻译： 通过使糖类与选自醌氧化还原辅因子ADH，烟酰胺腺嘌呤二核苷酸（NAD +）氧化还原辅因子ADH，烟酰胺腺嘌呤二核苷酸磷酸（NADP +））的醇脱氢酶（ADH）酶接触来进行氧化糖的方法， 描述了氧化还原辅因子依赖性ADH及其任何组合。 还描述了通过该方法获得的氧化糖，特别是含有氧化糖的食品和纸制品。
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公开(公告)号：US20110236935A1
公开(公告)日：2011-09-29
申请号：US12915853
申请日：2010-10-29
申请人： Jørn Dalgaard Mikkelsen , Karsten Matthias Kragh , Rene Mikkelsen , Patrick Maria Franciscus Derkx , Shukun Yu , Harm Mulder , Igor Nikolaev
发明人： Jørn Dalgaard Mikkelsen , Karsten Matthias Kragh , Rene Mikkelsen , Patrick Maria Franciscus Derkx , Shukun Yu , Harm Mulder , Igor Nikolaev
IPC分类号： C12P19/04 , C12P19/00 , C12P19/12 , C12P19/10 , C08B37/16 , C08B31/18 , C08B37/00 , C12N9/04 , C12N15/53 , C12N15/63 , C12N1/21 , C12N1/15 , C12N1/19
CPC分类号： C12N11/14 , C12N9/0006 , C12P19/00
摘要： A method of oxidising a saccharide, by contacting the saccharide with an alcohol dehydrogenase (ADH) enzyme selected from a quinone redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide (NAD+) redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide phosphate (NADP+) redox cofactor-dependent ADH, and any combination thereof is described. An oxidised saccharide obtainable by the method and products, in particular food products and paper products, containing the oxidised saccharide, are also described.
摘要翻译： 通过使糖与选自醌氧化还原辅因子ADH，烟酰胺腺嘌呤二核苷酸（NAD +）氧化还原辅因子依赖性ADH，烟酰胺腺嘌呤二核苷酸磷酸（NADP +）的NADP +）的醇脱氢酶（ADH）酶接触来进行氧化糖的方法， 描述了氧化还原辅因子依赖性ADH及其任何组合。 还描述了通过该方法获得的氧化糖，特别是含有氧化糖的食品和纸制品。
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公开(公告)号：US08663966B2
公开(公告)日：2014-03-04
申请号：US11857691
申请日：2007-09-19
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： We disclose a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises substitutions at the following positions: G121D, 134, 141, 157, 223, 307 and 334 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.
摘要翻译： 我们公开了包含PS4变体多肽的食品添加剂，其中PS4变体多肽可衍生自具有非麦芽糖外切酶活性的亲本多肽，其中PS4变体多肽包含以下位置的取代：G121D，134,141,157 ，223,307和334，参照SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列的位置编号。
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6.发明申请ENZYMATIC GENERATION OF FUNCTIONAL LIPIDS FROM CEREALS OR CEREAL BI-STREAMS 有权来自谷物或谷物双流的功能性脂肪酶的生物生成公开(公告)号：US20120003690A1
公开(公告)日：2012-01-05
申请号：US13144588
申请日：2010-01-15
IPC分类号： C12P33/00 , C07C31/08 , C12P7/64 , C12P9/00 , A21D8/04 , C12C5/02 , A23L1/05 , A23L1/10 , A23L1/16 , C07C29/00 , C07C53/126
CPC分类号： A21D8/042 , A23L7/107 , A23L7/115 , C11B1/025 , C12Y301/01001 , C12Y301/01004 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/01004 , C12Y302/01008 , C12Y302/01015 , C12Y302/0102 , C12Y302/01021 , C12Y302/01024 , C12Y302/01041 , C12Y302/01055 , C12Y302/0106 , C12Y302/01068 , C12Y302/01089 , C12Y302/01091 , C12Y302/01133 , Y02E50/17
摘要： The present invention relates to the modification of lipids in lipid-containing plant material, such as cereal bran for the generation of functional lipids. The present invention further relates to the preparation of compositions comprising such functional lipids as well as the use of these compositions comprising functional lipids and other functional compounds derived from the action of lipid modifying enzymes for the preparation of composition suitable for the preparation of bio-ethanol as well as food products, such as bread.
摘要翻译： 本发明涉及用于产生功能性脂质的含脂质植物材料如谷物麸皮中脂质的修饰。 本发明还涉及包含这种功能性脂质的组合物的制备以及这些组合物的使用，其包含衍生自脂质修饰酶作用的功能性脂质和其它功能性化合物，用于制备适于制备生物乙醇的组合物 以及食品，如面包。
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7.发明授权Thermostable amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 有权热稳定淀粉酶多肽，编码那些多肽的核酸及其用途公开(公告)号：US07776576B2
公开(公告)日：2010-08-17
申请号：US10886905
申请日：2004-07-07
申请人： Karsten Matthias Kragh , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
发明人： Karsten Matthias Kragh , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered thermostability.
摘要翻译： 本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。 本发明的淀粉酶已被设计成具有更多的有益品质。 具体地说，本发明的淀粉酶显示出改变的热稳定性。
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公开(公告)号：US20080227173A1
公开(公告)日：2008-09-18
申请号：US11534624
申请日：2006-09-22
申请人： Casper Tune BERG , Patrick Maria Franciscus Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Hemmingen Kellet-Smith , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Bo Spange Sorensen , Charlotte Refdahl Thoudahl
发明人： Casper Tune BERG , Patrick Maria Franciscus Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Hemmingen Kellet-Smith , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Bo Spange Sorensen , Charlotte Refdahl Thoudahl
IPC分类号： C12N9/28
CPC分类号： A21D8/042 , A23K20/147 , A23K50/30 , A23K50/75 , A23L33/18 , C12N9/2411 , C12N9/2414 , C12N9/2417 , C12N9/2425 , C12N9/2428
摘要： The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.
摘要翻译： 本发明描述了可衍生自具有选自以下的淀粉酶活性的亲本多肽的PS4变体多肽： 161,178,179,223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参照SEQ ID NO：1所示的假单胞菌属酵母胞外淀粉酶序列的位置编号，这种多肽作为食品或饲料添加剂的使用，以及编码这样的核酸。
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公开(公告)号：US07371552B2
公开(公告)日：2008-05-13
申请号：US10886023
申请日：2004-07-07
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： We disclose a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises substitutions at the following positions: G121D, 134, 141, 157, 223, 307 and 334 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.
摘要翻译： 我们公开了包含PS4变体多肽的食品添加剂，其中PS4变体多肽可衍生自具有非麦芽糖外切酶活性的亲本多肽，其中PS4变体多肽包含以下位置的取代：G121D，134,141,157 ，223,307和334，参照SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列的位置编号。
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公开(公告)号：US20120058222A1
公开(公告)日：2012-03-08
申请号：US13319426
申请日：2010-05-19
申请人： Bo Spange Sorensen , Inge Lise Povlsen , Rie Mejldal , Karsten Matthias Kragh , Anja Hemmingsen Kellett-Smith , Rene Mikkelsen , Rikke L. Bundgaard Jenner
发明人： Bo Spange Sorensen , Inge Lise Povlsen , Rie Mejldal , Karsten Matthias Kragh , Anja Hemmingsen Kellett-Smith , Rene Mikkelsen , Rikke L. Bundgaard Jenner
CPC分类号： C12N9/20 , A21D8/042 , C12N9/1029 , C12N9/2417
摘要： The present invention relates to the use of an amylase and a lipolytic enzyme in combination to improve the stackability of bread, methods of preparing dough and baked products having a combination of such enzymes, as well as bread having particular bread stackability profiles.
摘要翻译： 本发明涉及组合淀粉酶和脂肪分解酶以改善面包的可堆叠性的用途，制备面团的方法和具有这种酶的组合的烘焙产品，以及具有特定面包堆叠性的面包。
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