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165 条记录 (耗时 0.029 秒)

    Process for producing peptides in E. coli
    4.
    发明授权
    Process for producing peptides in E. coli 失效
    在大肠杆菌中生产肽的方法

    公开(公告)号：US5670340A

    公开(公告)日：1997-09-23

    申请号：US352179

    申请日：1994-12-05

    申请人： Masayuki Yabuta ,  Yuji Suzuki ,  Kazuhiro Ohsuye ,  Takehiro Oshima ,  Seiko Onai ,  Koji Magota ,  Shoji Tanaka

    发明人： Masayuki Yabuta ,  Yuji Suzuki ,  Kazuhiro Ohsuye ,  Takehiro Oshima ,  Seiko Onai ,  Koji Magota ,  Shoji Tanaka

    IPC分类号： C12N15/09 ,  C07K14/58 ,  C07K14/585 ,  C12N9/38 ,  C12N15/16 ,  C12N15/62 ,  C12N15/67 ,  C12N15/70 ,  C12P21/02 ,  C12P21/06 ,  C12R1/19 ,  C12P21/00

    CPC分类号： C07K14/58 ,  C07K14/585 ,  C12N15/62 ,  C12N9/2471 ,  C12Y302/01023 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/43 ,  C07K2319/50 ,  C07K2319/75 ,  C07K2319/90

    摘要： The present invention is a process to express a target peptide in a large amount and accumulate the target peptide in host cells in the form of inclusion bodies. The process comprises: A) culturing host cells transformed with a plasmid able to express a gene coding for a fusion protein represented in the formula A--L--B, wherein B is a target peptide, A is a protective peptide comprising a 90-210 amino acid fragment E. coli .beta.-galactosidase, and L is a linker peptide positioned between the C-terminus of the protective peptide and the N-terminus of the target peptide and selected so that when the fusion protein is treated by an enzyme or chemical substance, the target peptide is separated, and wherein the protective peptide and linker peptide are selected so that the isoelectric point of the fusion protein in between 4.9 and 6.9; B) obtaining an insoluble fraction comprising inclusion bodies by homogenization of th cultured transformed cells; C) solubilizing the fusion protein in the inclusion bodies by treatment of the insoluble fraction with a solubilizing agent; and, D) cleaving the peptide bond between the C-terminus of the linker peptide and the N-terminus of the target peptide of the solubilized fusion protein to release the target peptide from the other peptides followed by purification of the target peptide.

    摘要翻译： 本发明是大量表达目标肽并以包涵体的形式在宿主细胞中聚集靶肽的方法。 该方法包括：A）培养用能够表达编码式ALB中表示的融合蛋白的基因的质粒转化的宿主细胞，其中B是靶肽，A是包含90-210个氨基酸片段E的保护肽 大肠杆菌β-半乳糖苷酶，L是位于保护肽的C-末端和靶肽的N末端之间的连接肽，并且被选择使得当融合蛋白被酶或化学物质处理时，靶 分离肽，并且其中选择保护性肽和接头肽，使得融合蛋白的等电点在4.9和6.9之间; B）通过均质培养的转化细胞获得包含包含体的不溶性级分; C）通过用增溶剂处理不溶性级分将融合蛋白溶解在包涵体中; 并且D）切割接头肽的C末端和溶解的融合蛋白的靶肽的N末端之间的肽键，从其他肽释放靶肽，然后纯化目标肽。

    Precursor of a C-terminal amidated calcitonin
    7.
    发明授权
    Precursor of a C-terminal amidated calcitonin 失效
    C-末端酰胺化降钙素前体

    公开(公告)号：US5252482A

    公开(公告)日：1993-10-12

    申请号：US610312

    申请日：1990-11-05

    申请人： Shoji Tanaka ,  Kazuhiro Ohsuye ,  Ichiro Kubota ,  Norio Ohnuma ,  Teruhisa Noguchi

    发明人： Shoji Tanaka ,  Kazuhiro Ohsuye ,  Ichiro Kubota ,  Norio Ohnuma ,  Teruhisa Noguchi

    IPC分类号： A61K38/00 ,  C07K14/585 ,  C12N1/21 ,  C12N15/16 ,  C12N15/70

    CPC分类号： C07K14/585 ,  A61K38/00

    摘要： A precursor of a C-terminal amidated peptide represented by the general formula P-X-Gly-Y.sub.n, wherein P is a peptide residue. X is an amino acid residue the C terminal of which (on the Gly side) can be converted in vivo to a --CONH.sub.2 group. Gly is a glycine residue, Y is a basic amino acid residue, n is an interger of 2 to 4 and a further amino acid residue other than Y or a peptide residue may be attached to Y.sub.n, is produced by a gene engineering technology. The precursor exhibits in vivo physiological activity like the C-terminal amidated peptide.

    摘要翻译： 由通式P-X-Gly-Yn表示的C末端酰胺化肽的前体，其中P是肽残基。 X是其末端（Gly侧）可以在体内转化为-CONH 2基团的氨基酸残基。 Gly是甘氨酸残基，Y是碱性氨基酸残基，n是2至4的整数，并且除Y之外的另外的氨基酸残基或肽残基可以连接到Yn上，是通过基因工程技术产生的。 前体表现出体内生理活性，如C-末端酰胺化肽。

    High-toughness coating film and sliding member
    8.
    发明授权
    High-toughness coating film and sliding member 有权
    高韧性涂膜和滑动件

    公开(公告)号：US09416434B2

    公开(公告)日：2016-08-16

    申请号：US14385183

    申请日：2012-03-16

    申请人： Kiyoyuki Kawai ,  Shoji Tanaka

    发明人： Kiyoyuki Kawai ,  Shoji Tanaka

    IPC分类号： C23C14/06 ,  C22C27/06 ,  F16J9/26 ,  C23C14/32

    CPC分类号： C22C27/06 ,  C23C14/0641 ,  C23C14/32 ,  F16J9/26 ,  Y10T428/31678

    摘要： Provided is a high-toughness coating film having excellent wear resistance, in particular, resistance to cracking and peeling. A high-toughness coating film (2) includes a composition containing a mixture of metallic chromium, in which nitrogen is dissolved, and Cr2N. A content of the nitrogen in the high-toughness coating film is 2.0 to 8.5 mass %, and IP, which is expressed by total of each diffraction peak intensity of Cr2N/(total of each diffraction peak intensity of metallic chromium+total of each diffraction peak intensity of Cr2N), is 3 to 40% when 2θ is in a range of 30° to 90°, where θ is an incident angle in X-ray diffraction of the high-toughness coating film.

    摘要翻译： 提供具有优异的耐磨性，特别是耐开裂和剥离的高韧性涂膜。 高韧性涂膜（2）包括含有溶解有氮的金属铬与Cr 2 N的混合物的组合物。 高韧性涂膜中的氮含量为2.0〜8.5质量％，IP由Cr 2 N的衍射峰强度的总和（各衍射峰强度的金属铬的总和+各衍射的总和）表示 当2θ在30°至90°的范围内时，Cr为高于Cr 2 N的峰强度）为3〜40％，其中θ为高韧性涂膜的X射线衍射中的入射角。

    DATA PROCESSING DEVICE
    9.
    发明申请
    DATA PROCESSING DEVICE 审中-公开
    数据处理设备

    公开(公告)号：US20120271830A1

    公开(公告)日：2012-10-25

    申请号：US13516438

    申请日：2009-12-16

    申请人： Tomohiro Shiino ,  Yoko Sano ,  Tsuyoshi Sempuku ,  Hideto Miyazaki ,  Kuniyo Ieda ,  Takashi Sadahiro ,  Shoji Tanaka

    发明人： Tomohiro Shiino ,  Yoko Sano ,  Tsuyoshi Sempuku ,  Hideto Miyazaki ,  Kuniyo Ieda ,  Takashi Sadahiro ,  Shoji Tanaka

    IPC分类号： G06F17/30

    CPC分类号： G06F16/64 ,  G06F16/68

    摘要： Disclosed is a data processing device including a data acquisition unit for acquiring data from a medium, an integrated database for integrating the data acquired by the data acquisition unit thereinto, a data analysis determination unit for analyzing the data integrated into the integrated database, a display control unit for creating an image of a side view of and an image of a bottom view of a solid expressing the data integrated into the integrated database on the basis of an analysis result acquired by the data analysis determination unit and the data, and a display unit for displaying the images created by the display control unit.

    摘要翻译： 公开了一种数据处理装置，包括：用于从介质获取数据的数据获取单元，用于将由数据获取单元获取的数据集成到其中的综合数据库，用于分析集成到集成数据库中的数据的数据分析确定单元， 控制单元，用于基于由数据分析确定单元获取的分析结果和数据来创建表示集成到集成数据库中的数据的实体的侧视图和底视图的图像;以及显示器 用于显示由显示控制单元创建的图像的单元。

    NAVIGATION APPARATUS
    10.
    发明申请
    NAVIGATION APPARATUS 有权
    导航装置

    公开(公告)号：US20120109516A1

    公开(公告)日：2012-05-03

    申请号：US13266035

    申请日：2009-12-08

    申请人： Hideto Miyazaki ,  Tsuyoshi Sempuku ,  Shoji Tanaka ,  Kuniyo Ieda ,  Takashi Sadahiro

    发明人： Hideto Miyazaki ,  Tsuyoshi Sempuku ,  Shoji Tanaka ,  Kuniyo Ieda ,  Takashi Sadahiro

    IPC分类号： G01C21/36

    CPC分类号： G09B29/102 ,  G01C21/367

    摘要： A navigation apparatus is provided that is highly convenient for a user to view a map screen and a menu window. A display controller 15 determines a view mode of each of a map screen and a menu window, based on information about a travel condition of a moving object from a route search unit 12 and an own-vehicle location identification unit, when the menu window is displayed according to information entered from an input unit 10. A display map generator 16 generates a map screen according to information about the view mode from the display controller 15. A display menu generator 17 generates a menu window according to information about the view mode from the display controller 15. A display unit 21 displays the map screen from the display map generator 16 and the menu window from the menu generator 17.

    摘要翻译： 提供了一种导航装置，其非常方便用户查看地图屏幕和菜单窗口。 显示控制器15基于关于来自路线搜索单元12和本车位置识别单元的移动物体的行驶状况的信息，在菜单窗口为“否”的情况下，确定地图画面和菜单窗口中的每一个的视图模式 根据从输入单元10输入的信息显示。显示图生成器16根据来自显示控制器15的关于视图模式的信息生成地图画面。显示菜单生成器17根据关于视图模式的信息生成菜单窗口 显示控制器15.显示单元21从显示图生成器16显示地图画面和菜单生成器17的菜单窗口。