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    PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE HISTIDINE BIOSYNTHETIC PATHWAY
    2.
    发明申请
    PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE HISTIDINE BIOSYNTHETIC PATHWAY 审中-公开
    PIC IA IA IS AY I AY AY AY AY AY AY AY AY AY AY AY AY AY AY AY AY

    公开(公告)号：US20120100618A1

    公开(公告)日：2012-04-26

    申请号：US13272570

    申请日：2011-10-13

    申请人： Juergen Nett

    发明人： Juergen Nett

    IPC分类号： C12N15/81 ,  C12N15/52 ,  C12N1/19

    CPC分类号： C12N15/52 ,  C12N9/88 ,  C12N15/815 ,  C12Y402/01019

    摘要： Disclosed is the HIS7 gene encoding the His7p enzyme in the histidine biosynthesis pathway of Pichia pastoris. The locus in the Pichia pastoris genome encoding the His7p is useful sites for stable integration of heterologous nucleic acid molecules into the Pichia pastoris genome. The gene or gene fragment encoding the His7p may be useful as a selection marker for constructing recombinant Pichia pastoris.

    摘要翻译： 公开了编码巴斯德毕赤酵母组氨酸生物合成途径中His7p酶的HIS7基因。 编码His7p的巴斯德毕赤酵母基因组中的位点是将异源核酸分子稳定整合到巴斯德毕赤酵母基因组中的有用位点。 编码His7p的基因或基因片段可用作构建重组巴斯德毕赤酵母的选择标记。

    METHODS FOR IMPROVING AND MANAGING NUCLEOSIDE REVERSE-TRANSCRIPTASE INHIBITION BASED TREATMENT
    4.
    发明申请
    METHODS FOR IMPROVING AND MANAGING NUCLEOSIDE REVERSE-TRANSCRIPTASE INHIBITION BASED TREATMENT 有权
    改进和管理核苷酸逆转录酶抑制基因治疗方法

    公开(公告)号：US20140141461A1

    公开(公告)日：2014-05-22

    申请号：US14118267

    申请日：2012-05-16

    申请人： Pablo Gluschankof ,  Christele Perrin-East

    发明人： Pablo Gluschankof ,  Christele Perrin-East

    IPC分类号： C12Q1/48

    CPC分类号： C12Q1/485 ,  C07K14/705 ,  C12N9/1205 ,  C12N9/1276 ,  C12N9/88 ,  C12N15/81 ,  C12Y207/01074 ,  C12Y207/07049 ,  C12Y402/01019

    摘要： A transformed yeast cell includes: a nucleic acid sequence coding for a reverse transcriptase; a reverse transcription indicator; a nucleic acid sequence coding for deoxycytidine kinase (dCK); and at least one nucleic acid sequence coding for nucleoside transporter. Methods for screening a compound with the ability to inhibit reverse transcription and for predicting the sensitivity of a reverse transcriptase to a NRTI compound, particularly a reverse transcriptase derived from a virus infecting a subject using the transformed Yeast cell are also described.

    摘要翻译： 转化的酵母细胞包括：编码逆转录酶的核酸序列; 逆转录指标; 编码脱氧胞苷激酶（dCK）的核酸序列; 和至少一个编码核苷转运蛋白的核酸序列。 还描述了筛选具有抑制逆转录能力的化合物和用于预测逆转录酶对NRTI化合物，特别是衍生自使用转化的酵母细胞感染受试者的病毒的逆转录酶的敏感性的方法。

    Methylotrophic yeast producing mammalian type sugar chain
    5.
    发明授权
    Methylotrophic yeast producing mammalian type sugar chain 有权
    甲基营养酵母产生哺乳动物型糖链

    公开(公告)号：US07972809B2

    公开(公告)日：2011-07-05

    申请号：US10511436

    申请日：2003-04-28

    申请人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasunori Chiba

    发明人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasunori Chiba

    IPC分类号： C12P21/00 ,  C12N15/01

    CPC分类号： C12P19/26 ,  C07K16/243 ,  C07K2317/21 ,  C12N1/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/2488 ,  C12N9/60 ,  C12N9/88 ,  C12N9/93 ,  C12P21/02 ,  C12Y101/01085 ,  C12Y101/03013 ,  C12Y102/01012 ,  C12Y302/01024 ,  C12Y402/01019 ,  C12Y603/02006

    摘要： This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an α-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the α-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity. By introducing a mammalian type sugar chain biosynthesizing gene into the above-described mutant, a mammalian type sugar chain, such as a hybrid or complex, or a protein comprising a mammalian type sugar chain can be efficiently produced.

    摘要翻译： 本发明提供一种生产包含哺乳动物型糖链的糖蛋白的方法，其特征在于该方法包括将α-1,2-甘露糖苷酶基因导入具有糖链生物合成酶基因突变的甲基营养酵母中，因此 α-1,2-甘露糖苷酶基因在酵母中有效启动子的控制下表达; 在介质中培养具有转移到其中的异源基因的甲基营养酵母细胞; 从培养物中获得包含哺乳动物型糖链的糖蛋白。 使用新制造的具有糖链突变的甲基营养酵母，可以大量生产与由哺乳动物细胞如人细胞产生的高甘露糖型糖链或包含这种中性糖链的糖蛋白相同的中性糖链 纯度高。 通过将哺乳动物型糖链生物合成基因导入到上述突变体中，可以有效地制备哺乳动物型糖链，例如杂合体或复合物，或包含哺乳动物型糖链的蛋白质。

    Methylotroph producing mammalian type sugar chain
    6.
    发明申请
    Methylotroph producing mammalian type sugar chain 有权
    生产甲基鱼油的哺乳动物类型的糖链

    公开(公告)号：US20060148039A1

    公开(公告)日：2006-07-06

    申请号：US10511436

    申请日：2003-04-28

    申请人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasumori Chiba

    发明人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasumori Chiba

    IPC分类号： C12P19/28 ,  C12N15/74 ,  C12N1/18

    CPC分类号： C12P19/26 ,  C07K16/243 ,  C07K2317/21 ,  C12N1/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/2488 ,  C12N9/60 ,  C12N9/88 ,  C12N9/93 ,  C12P21/02 ,  C12Y101/01085 ,  C12Y101/03013 ,  C12Y102/01012 ,  C12Y302/01024 ,  C12Y402/01019 ,  C12Y603/02006

    摘要： This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an α-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the α-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity. By introducing a mammalian type sugar chain biosynthesizing gene into the above-described mutant, a mammalian type sugar chain, such as a hybrid or complex, or a protein comprising a mammalian type sugar chain can be efficiently produced.

    摘要翻译： 本发明提供一种生产包含哺乳动物型糖链的糖蛋白的方法，其特征在于该方法包括将α-1,2-甘露糖苷酶基因导入具有糖链生物合成酶基因突变的甲基营养酵母中，因此 α-1,2-甘露糖苷酶基因在酵母中有效启动子的控制下表达; 在介质中培养具有转移到其中的异源基因的甲基营养酵母细胞; 从培养物中获得包含哺乳动物型糖链的糖蛋白。 使用新制造的具有糖链突变的甲基营养酵母，可以大量生产与由哺乳动物细胞如人细胞产生的高甘露糖型糖链或包含这种中性糖链的糖蛋白相同的中性糖链 纯度高。 通过将哺乳动物型糖链生物合成基因导入到上述突变体中，可以有效地制备哺乳动物型糖链，例如杂合体或复合物，或包含哺乳动物型糖链的蛋白质。