-
1.发明申请Environmental stress-responsive promoter and genes encoding transcriptional factor 审中-公开环境胁迫响应启动子和编码转录因子的基因公开(公告)号：US20050009187A1
公开(公告)日：2005-01-13
申请号：US10495918
申请日：2002-11-15
申请人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
发明人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
IPC分类号： A01H5/00 , C07K14/415 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/82 , C07H21/04 , C12N15/63 , C12N15/87
CPC分类号： C07K14/415 , C12N15/8237 , C12N15/8238 , C12N15/8273
摘要： The present invention provides a stress responsive promoter. The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c):(a) DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90; (b) DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter.
摘要翻译： 本发明提供一种应激反应启动子。 本发明的环境胁迫响应启动子包含以下（a），（b）或（c）的DNA：（a）由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA; （b）由相对于选自SEQ ID NO：1至90的任何核苷酸序列缺失，取代或添加一个或多个核苷酸的核苷酸序列组成的DNA，并且起到环境胁迫响应启动子的作用; 和（c）在严格条件下与由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA杂交并用作环境胁迫响应启动子的DNA。
-
2.发明申请ENVIRONMENTAL STRESS-RESPONSIVE PROMOTER AND A GENE ENCODING ENVIRONMENTAL STRESS-RESPONSIVE TRANSCRIPTIONAL FACTOR 失效环境胁迫响应性促进剂和基因编码环境胁迫响应性转录因子公开(公告)号：US20070094752A1
公开(公告)日：2007-04-26
申请号：US11470154
申请日：2006-09-05
申请人： Kazuo SHINOZAKI , Motoaki Seki , Miki Fujita
发明人： Kazuo SHINOZAKI , Motoaki Seki , Miki Fujita
IPC分类号： C12N15/82
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8237 , C12N15/8273
摘要： The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c): DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90; DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter.
摘要翻译： 本发明的环境胁迫响应启动子包含以下（a），（b）或（c）的DNA：由选自SEQ ID NO：1〜90的任意碱基序列组成的DNA; 由包含相对于选自SEQ ID NO：1至90的任何核苷酸序列的一个或多个核苷酸的缺失，取代或添加的核苷酸序列组成的DNA，并且起到环境应激反应启动子的作用; 和（c）在严格条件下与由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA杂交并用作环境胁迫响应启动子的DNA。
-
3.发明申请Environmental stress-responsive promoter and a gene encoding environmental stress-responsive transcriptional factor 审中-公开环境应激反应启动子和编码环境应激反应转录因子的基因公开(公告)号：US20080216196A1
公开(公告)日：2008-09-04
申请号：US12076932
申请日：2008-03-25
申请人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
发明人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8237 , C12N15/8273
摘要： The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.
摘要翻译： 本发明提供一种通过（a）用具有SEQ ID NO：27的非生物环境胁迫应答启动子和其下游基因的表达载体制备重组植物细胞系，植物组织或植物来调节基因表达的方法 ; 和（b）在非生物环境胁迫下培养和培养重组植物细胞，植物组织或植物，其中所述启动子调节所述基因在非生物环境胁迫下的表达。
-
4.发明申请ENVIRONMENTAL STRESS RESPONSIVE PROMOTER AND METHOD OF TISSUE-SPECIFIC GENE EXPRESSION USING THE SAME 审中-公开环境应激反应促进剂及使用其组织特异性基因表达的方法公开(公告)号：US20090282582A1
公开(公告)日：2009-11-12
申请号：US12311900
申请日：2007-10-19
申请人： Motoaki Seki , Jong-Myong Kim , Kazuo Shinozaki , Miki Fujita
发明人： Motoaki Seki , Jong-Myong Kim , Kazuo Shinozaki , Miki Fujita
CPC分类号： C07K14/415 , C12N15/8237
摘要： This invention concerns induction of gene expression specifically in given tissue with the use of a novel promoter that has environmental stress responsiveness and functions specifically in given tissue. It is realized by a method comprising: a step of preparing a plant having an arbitrary gene downstream of the environmental stress responsive promoter comprising DNA (a), (b), or (c) below; (a) DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8; (b) DNA comprising a nucleotide sequence derived from the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 by deletion, substitution, or addition of one or a plurality of nucleotides and functioning as an environmental stress responsive promoter; or (c) DNA hybridizing under stringent conditions to DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 and functioning as an environmental stress responsive promoter; and a step of cultivating the plant in the presence of environmental stress, the method comprising inducing expression of the gene located downstream of the environmental stress responsive promoter in a tissue-specific manner.
摘要翻译： 本发明涉及使用具有环境应激反应性和特定于给定组织中功能的新型启动子来特异性诱导给定组织中的基因表达。 通过以下方法实现：包括下述DNA（a），（b）或（c）的环境胁迫响应启动子下游具有任意基因的植物的步骤; （a）包含SEQ ID NO：1至8中任一个所示的核苷酸序列的DNA; （b）通过缺失，取代或添加一个或多个核苷酸并起到环境应激反应启动子的作用，其包含衍生自SEQ ID NO：1至8中任一项所示核苷酸序列的核苷酸序列的DNA; 或（c）在严格条件下与包含SEQ ID NO：1至8中任一所示的核苷酸序列的DNA杂交并用作环境胁迫响应启动子的DNA的DNA; 以及在存在环境胁迫的情况下培育植物的步骤，所述方法包括以组织特异性方式诱导位于环境胁迫响应启动子下游的基因的表达。
-
5.发明授权Plant system for comprehensive gene function analysis with the use of full-length cDNA 失效使用全长cDNA进行综合基因功能分析的植物系统公开(公告)号：US07608758B2
公开(公告)日：2009-10-27
申请号：US10488087
申请日：2002-08-29
申请人： Minami Matsui , Takanari Ichikawa , Miki Nakazawa , Motoaki Seki , Miki Fujita , Kazuo Shinozaki
发明人： Minami Matsui , Takanari Ichikawa , Miki Nakazawa , Motoaki Seki , Miki Fujita , Kazuo Shinozaki
IPC分类号： C12N15/82
CPC分类号： C12N15/8216
摘要： A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.
摘要翻译： 一个易于指定表型的基因，从而全面分析基因功能。 用于分析基因功能的方法包括（a）用含有表达调控序列的全长cDNA文库感染植物组，以将cDNA引入植物中; （b）从已经引入cDNA的植物中选择符合所需选择要求的植物; （c）从所选择的植物中分离cDNA; 和（d）将如此分离的cDNA重新引入与引入cDNA的植物相同物种的植物中，以根据选择要求重新确认表型。
-
公开(公告)号：US07368630B2
公开(公告)日：2008-05-06
申请号：US11470154
申请日：2006-09-05
申请人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
发明人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8237 , C12N15/8273
摘要： The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.
-
公开(公告)号：US20070006348A1
公开(公告)日：2007-01-04
申请号：US09988739
申请日：2001-11-20
申请人： Kazuo Shinozaki , Motoaki Seki , Tokihiko Nanjo
发明人： Kazuo Shinozaki , Motoaki Seki , Tokihiko Nanjo
CPC分类号： C12N15/8273 , A01K2217/05 , C12N15/8237
摘要： The present invention provides a stress responsive promoter. The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c): (a) DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 18; (b) DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 18, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 18, and functioning as an environmental stress responsive promoter.
摘要翻译： 本发明提供一种应激反应启动子。 本发明的环境胁迫响应启动子包含以下（a），（b）或（c）的DNA：（a）由选自SEQ ID NO：1至18的任何核苷酸序列组成的DNA; （b）由相对于选自SEQ ID NO：1至18的任何核苷酸序列的缺失，取代或添加一个或多个核苷酸的核苷酸序列组成并用作环境胁迫响应启动子的DNA; 和（c）在严格条件下与由选自SEQ ID NO：1至18的任何核苷酸序列组成的DNA杂交并用作环境胁迫应答启动子的DNA。
-
8.发明申请Process for producing ligand binding protein with the use of cell-free protein synthesis system and utilization thereof 审中-公开使用无细胞蛋白质合成系统产生配体结合蛋白的方法及其利用公开(公告)号：US20050221356A1
公开(公告)日：2005-10-06
申请号：US11081914
申请日：2005-03-17
申请人： Takanori Kigawa , Takayoshi Matsuda , Masaaki Aoki , Shigeyuki Yokoyama , Kazuo Shinozaki , Motoaki Seki
发明人： Takanori Kigawa , Takayoshi Matsuda , Masaaki Aoki , Shigeyuki Yokoyama , Kazuo Shinozaki , Motoaki Seki
IPC分类号： G01N33/50 , C12N15/09 , C12P19/34 , C12P21/00 , C12P21/02 , C12P21/06 , C12Q1/00 , C12Q1/68 , G01N33/15 , G01N33/53 , G01N33/566
CPC分类号： C12P21/02 , G01N2500/04
摘要： It is intended to provide a process for efficiently producing a ligand binding protein characterized in that this protein or its domain is synthesized in a cell-free protein synthesis system containing a ligand that binds to the protein or its domain. In a preferred embodiment, the above-described ligand is a substance selected from the group consisting of DNAs, RNAs, nucleosides, nucleotides, proteins, peptides, aminoacids, lipids, carbohydrates, derivatives thereof, alkaloids, terpenes, low-molecular weight compounds, coenzymes, low-molecular weight metal complexes and metal ions, while the ligand binding protein or the ligand binding domain thereof is synthesized as a soluble protein.
摘要翻译： 旨在提供一种有效产生配体结合蛋白的方法，其特征在于该蛋白质或其结构域在含有与蛋白质或其结构域结合的配体的无细胞蛋白质合成系统中合成。 在优选的实施方案中，上述配体是选自DNA，RNA，核苷，核苷酸，蛋白质，肽，氨基酸，脂质，碳水化合物，其衍生物，生物碱，萜烯，低分子量化合物， 辅酶，低分子金属络合物和金属离子，而配体结合蛋白或其配体结合结构域作为可溶性蛋白质合成。
-
公开(公告)号：US09258954B2
公开(公告)日：2016-02-16
申请号：US13411318
申请日：2012-03-02
申请人： Motoaki Seki , Taiko To , Jong-Myong Kim
发明人： Motoaki Seki , Taiko To , Jong-Myong Kim
CPC分类号： A01H3/04 , C12N9/16 , C12N15/8273
摘要： An object of the present invention is to provide a plant having enhanced resistance to environmental stress by controlling the expression of a histone modifying enzyme in a plant. The present invention relates to a method of producing a plant having enhanced resistance to environmental stress, comprising a step of suppressing or increasing the expression of a histone deacetylase gene.
摘要翻译： 本发明的目的是通过控制植物中组蛋白修饰酶的表达来提供具有增强的耐环境胁迫性的植物。 本发明涉及具有增强的抗环境胁迫性的植物的生产方法，其包括抑制或增加组蛋白脱乙酰酶基因表达的步骤。
-
公开(公告)号：US20120227135A1
公开(公告)日：2012-09-06
申请号：US13411318
申请日：2012-03-02
申请人： Motoaki Seki , Taiko To , Jong-Myong Kim
发明人： Motoaki Seki , Taiko To , Jong-Myong Kim
CPC分类号： A01H3/04 , C12N9/16 , C12N15/8273
摘要： An object of the present invention is to provide a plant having enhanced resistance to environmental stress by controlling the expression of a histone modifying enzyme in a plant. The present invention relates to a method of producing a plant having enhanced resistance to environmental stress, comprising a step of suppressing or increasing the expression of a histone deacetylase gene.
摘要翻译： 本发明的目的是通过控制植物中组蛋白修饰酶的表达来提供具有增强的耐环境胁迫性的植物。 本发明涉及具有增强的抗环境胁迫性的植物的生产方法，其包括抑制或增加组蛋白脱乙酰酶基因表达的步骤。
-
-
-
-
-
-
-
-
-
搜索结果
12 条记录 (耗时 0.019 秒)
