公开(公告)号：US20060035382A1

公开(公告)日：2006-02-16

申请号：US11083103

申请日：2005-03-18

申请人： Kazuo Shinozaki ,  Takashi Hirayama ,  Jun Kikuchi ,  Takashi Nishihara

发明人： Kazuo Shinozaki ,  Takashi Hirayama ,  Jun Kikuchi ,  Takashi Nishihara

IPC分类号： G01N24/00

CPC分类号： G01R33/465 ,  G01N24/08 ,  Y10T436/13 ,  Y10T436/24

摘要： The present invention provides a novel NMR metabolomics method for observing an animal by labeling the animal itself. A labeled animal labeled with a stable isotope is produced by feeding an animal a labeled food labeled with the stable isotope. Metabolism of a biological substance in the animal is analyzed based on nuclear magnetic resonance data of the biological substance containing the stable isotope. The nuclear magnetic resonance data is acquired by performing NMR measurement on a body of the labeled animal, a portion of the body, or an extract.

摘要翻译： 本发明提供了一种用于通过标记动物本身来观察动物的新型NMR代谢组学方法。 标记有稳定同位素的标记动物通过向动物喂养用稳定同位素标记的标记食物来产生。 基于含有稳定同位素的生物物质的核磁共振数据分析动物中生物物质的代谢。 核磁共振数据是通过对标记的动物的身体，身体的一部分或提取物进行核磁共振测量而获得的。

公开(公告)号：US20060035210A1

公开(公告)日：2006-02-16

申请号：US11083357

申请日：2005-03-18

申请人： Kazuo Shinozaki ,  Takashi Hirayama ,  Jun Kikuchi

发明人： Kazuo Shinozaki ,  Takashi Hirayama ,  Jun Kikuchi

IPC分类号： C12Q1/00

CPC分类号： A01G7/00

摘要： The present invention provides a method of analyzing metabolism of a plant in which measurement sensitivity for nuclides such as 13C and 15N having low natural abundance ratios is improved using NMR. A labeled plant uniformly labeled with a stable isotope is produced by providing the plant with a nutrition source labeled with at least one type of stable isotope while growing the plant from a seed or a germinating seedling. The metabolism of a biological substance in the plant is analyzed based on NMR data of a biological substance that contains the stable isotope. The NMR data is acquired by performing NMR measurement on a body of the labeled plant, a portion of the body, or an extract.

摘要翻译： 本发明提供了一种分析植物代谢的方法，其中使用NMR改善了具有低天然丰度比的诸如13 C和15 N之类的核素的测量灵敏度。 通过向植物提供标记有至少一种类型的稳定同位素的营养源，同时从种子或发芽幼苗生长植物，产生用稳定同位素均匀标记的标记植物。 基于含有稳定同位素的生物物质的NMR数据分析植物中生物物质的代谢。 通过对标记植物的身体，身体的一部分或提取物进行核磁共振测量来获得NMR数据。

公开(公告)号：US07482509B2

公开(公告)日：2009-01-27

申请号：US11385832

申请日：2006-03-22

申请人： Satoshi Iuchi ,  Masatomo Kobayashi ,  Kazuo Shinozaki

发明人： Satoshi Iuchi ,  Masatomo Kobayashi ,  Kazuo Shinozaki

IPC分类号： C12N15/82 ,  C12N15/29 ,  A01H5/00

CPC分类号： C12N9/0071 ,  C12N15/825 ,  C12N15/8273

摘要： A DNA encoding a neoxanthin cleavage enzyme used for improving stress tolerance in a plant, a method for increasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which a neoxanthin cleavage enzyme gene is introduced, are provided. A DNA used for reducing stress tolerance in a plant, a method for decreasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which the DNA is introduced, are also provided. The present invention enables creating a plant in which stress tolerance has been increased or decreased.

公开(公告)号：US07214856B2

公开(公告)日：2007-05-08

申请号：US10994235

申请日：2004-11-23

申请人： Taishi Umezawa ,  Kazuo Shinozaki

发明人： Taishi Umezawa ,  Kazuo Shinozaki

IPC分类号： C12N15/82 ,  C12N15/90

CPC分类号： C12N15/8273 ,  C12N9/12 ,  C12N15/8271

摘要： A stress-tolerant transgenic plant comprising an exogenously-introduced polynucleotide encoding a protein kinase which activates stress-responsive transcription factors. The polynucleotide may encode a protein kinase which comprises SEQ ID NO: 2 (SRK-2C) or a sequence having a significant degree of similarity with SEQ ID NO: 2. The transgenic plant has increased stress-tolerance to environmental stresses, such as to dehydration, osmotic stress or low temperature stress, compared to a similar non-transgenic plant.

摘要翻译： 一种耐应力转基因植物，其包含外源导入的多核苷酸，其编码蛋白激酶，其激活应激反应转录因子。 多核苷酸可编码包含SEQ ID NO：2（SRK-2C）或与SEQ ID NO：2具有显着程度相似性的序列的蛋白激酶。转基因植物具有增加的对环境胁迫的胁迫耐受性，例如 脱水，渗透胁迫或低温胁迫，与类似的非转基因植物相比。

公开(公告)号：US07049487B2

公开(公告)日：2006-05-23

申请号：US09758269

申请日：2001-01-12

申请人： Satoshi Iuchi ,  Masatomo Kobayashi ,  Kazuo Shinozaki

发明人： Satoshi Iuchi ,  Masatomo Kobayashi ,  Kazuo Shinozaki

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N9/0071 ,  C12N15/825 ,  C12N15/8273

摘要： A DNA encoding a neoxanthin cleavage enzyme used for improving stress tolerance in a plant, a method for increasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which a neoxanthin cleavage enzyme gene is introduced, are provided. A DNA used for reducing stress tolerance in a plant, a method for decreasing stress tolerance in a plant by introducing the DNA into the plant, and a transgenic plant into which the DNA is introduced, are also provided. The present invention enables creating a plant in which stress tolerance has been increased or decreased.

公开(公告)号：US20050009187A1

公开(公告)日：2005-01-13

申请号：US10495918

申请日：2002-11-15

申请人： Kazuo Shinozaki ,  Motoaki Seki ,  Miki Fujita

发明人： Kazuo Shinozaki ,  Motoaki Seki ,  Miki Fujita

IPC分类号： A01H5/00 ,  C07K14/415 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/82 ,  C07H21/04 ,  C12N15/63 ,  C12N15/87

CPC分类号： C07K14/415 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8273

摘要： The present invention provides a stress responsive promoter. The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c):(a) DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90; (b) DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter.

摘要翻译： 本发明提供一种应激反应启动子。 本发明的环境胁迫响应启动子包含以下（a），（b）或（c）的DNA：（a）由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA; （b）由相对于选自SEQ ID NO：1至90的任何核苷酸序列缺失，取代或添加一个或多个核苷酸的核苷酸序列组成的DNA，并且起到环境胁迫响应启动子的作用; 和（c）在严格条件下与由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA杂交并用作环境胁迫响应启动子的DNA。

公开(公告)号：US20120272401A1

公开(公告)日：2012-10-25

申请号：US13518303

申请日：2010-12-21

申请人： Takashi Kuromori ,  Kazuo Shinozaki

发明人： Takashi Kuromori ,  Kazuo Shinozaki

IPC分类号： A01H5/00 ,  A01H5/10 ,  A01H1/00 ,  C12N5/10

CPC分类号： C12N15/8271 ,  C07K14/415 ,  C12N15/8262

摘要： The present invention relates to a transgenic plant tolerant to environmental stress that comprises DNA encoding an exogenous abscisic acid (ABA) transporter protein in an expressible manner, a progeny thereof, or a cell, tissue or seed from such plant. The present invention also relates to a method for producing such a plant.

摘要翻译： 本发明涉及耐受环境胁迫的转基因植物，其包含以可表达的方式编码外源脱落酸（ABA）转运蛋白的DNA，其后代或来自该植物的细胞，组织或种子。 本发明还涉及这种植物的制造方法。

公开(公告)号：US20070174937A1

公开(公告)日：2007-07-26

申请号：US11677428

申请日：2007-02-21

申请人： Taishi Umezawa ,  Kazuo Shinozaki

发明人： Taishi Umezawa ,  Kazuo Shinozaki

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/415

CPC分类号： C12N15/8273 ,  C12N9/12 ,  C12N15/8271

摘要： This invention provides a stress tolerant transgenic plant with the utilization of a signal transduction factor located upstream of the gene group associated with environmental responses. This invention provides a gene encoding the following protein (a) or (b) and a stress tolerant transgenic plant to which such gene has been introduced: (a) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 2 in the Sequence Listing; or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 2 in the Sequence Listing by deletion. substitution, or addition of one or several amino acid residues and having activity of inducing expression of stress responsive transcription factors and activity of protein kinase.

摘要翻译： 本发明提供了一种利用位于与环境反应相关的基因组上游的信号转导因子的胁迫耐受转基因植物。 本发明提供了编码以下蛋白质（a）或（b）的基因和已经引入该基因的胁迫耐受性转基因植物：（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质 序列表; 或（b）由序列表中SEQ ID NO：2所示的氨基酸序列的氨基酸序列构成的蛋白质，通过缺失。 取代或添加一个或几个氨基酸残基，并具有诱导应激反应转录因子表达和蛋白激酶活性的活性。

公开(公告)号：US20060200876A1

公开(公告)日：2006-09-07

申请号：US11405477

申请日：2006-04-18

申请人： Chieko Ohsumi ,  Teruaki Taji ,  Kazuo Shinozaki

发明人： Chieko Ohsumi ,  Teruaki Taji ,  Kazuo Shinozaki

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N9/1051 ,  C12N15/8271 ,  C12N15/8273

摘要： The present invention enables production of a plant resistant to environmental stresses including drought stress. The invention increases stress resistance to a plant by increasing raffinose content in the plant.

公开(公告)号：US20050221356A1

公开(公告)日：2005-10-06

申请号：US11081914

申请日：2005-03-17

申请人： Takanori Kigawa ,  Takayoshi Matsuda ,  Masaaki Aoki ,  Shigeyuki Yokoyama ,  Kazuo Shinozaki ,  Motoaki Seki

发明人： Takanori Kigawa ,  Takayoshi Matsuda ,  Masaaki Aoki ,  Shigeyuki Yokoyama ,  Kazuo Shinozaki ,  Motoaki Seki

IPC分类号： G01N33/50 ,  C12N15/09 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02 ,  C12P21/06 ,  C12Q1/00 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/53 ,  G01N33/566

CPC分类号： C12P21/02 ,  G01N2500/04

摘要： It is intended to provide a process for efficiently producing a ligand binding protein characterized in that this protein or its domain is synthesized in a cell-free protein synthesis system containing a ligand that binds to the protein or its domain. In a preferred embodiment, the above-described ligand is a substance selected from the group consisting of DNAs, RNAs, nucleosides, nucleotides, proteins, peptides, aminoacids, lipids, carbohydrates, derivatives thereof, alkaloids, terpenes, low-molecular weight compounds, coenzymes, low-molecular weight metal complexes and metal ions, while the ligand binding protein or the ligand binding domain thereof is synthesized as a soluble protein.

摘要翻译： 旨在提供一种有效产生配体结合蛋白的方法，其特征在于该蛋白质或其结构域在含有与蛋白质或其结构域结合的配体的无细胞蛋白质合成系统中合成。 在优选的实施方案中，上述配体是选自DNA，RNA，核苷，核苷酸，蛋白质，肽，氨基酸，脂质，碳水化合物，其衍生物，生物碱，萜烯，低分子量化合物， 辅酶，低分子金属络合物和金属离子，而配体结合蛋白或其配体结合结构域作为可溶性蛋白质合成。