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公开(公告)号：US07238857B2
公开(公告)日：2007-07-03
申请号：US10503261
申请日：2003-02-06
申请人： Ken-ichi Yamazaki , Susumu Hirose
发明人： Ken-ichi Yamazaki , Susumu Hirose
CPC分类号： C07K14/415 , C12N15/8238
摘要： An ethylene-responsive transcription co-activator (MBF) acts on ethylene-responsive transcription factors (ERFs), which positively controls the expression of ethylene-responsive plant genes. A plant is transformed by the gene of the co-activator, and a promoter that may increase or decrease responsiveness to ethylene depending on its alignment. A method for controlling the responsiveness of plants to ethylene transforms a plant comprising an ERF-dependent gene, which positively control the expression of ethylene responsive genes of plants. This transcription co-activator gene is applied to control ethylene response of plants.
摘要翻译： 乙烯应答转录共激活子（MBF）作用于乙烯应答转录因子（ERFs），其正向控制乙烯应答植物基因的表达。 植物由共活化剂的基因转化，并且可以根据其对准而增加或减少对乙烯的响应性的启动子。 用于控制植物对乙烯的响应性的方法将包含ERF依赖性基因的植物转化，所述基因能正确控制植物的乙烯应答基因的表达。 该转录共活化基因用于控制植物的乙烯应答。
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公开(公告)号：US20050132443A1
公开(公告)日：2005-06-16
申请号：US10503261
申请日：2003-02-06
申请人： Ken-ichi Yamazaki , Susumu Hirose
发明人： Ken-ichi Yamazaki , Susumu Hirose
CPC分类号： C07K14/415 , C12N15/8238
摘要： The purpose of the present invention is to identify a transcription coactivator (transcriptional cofactor, MBF) in ethylene-responsive transcription factors (ERFs), to elucidate the mechanism of the action of MBF to ERF, which positively controls the expression of ethylene-responsive genes of plants, and furthermore, to provide a method for controlling the ethylene response of plants. An ethylene-responsive transcription coactivator (SEQ ID NO: 1 and 2) was identified. The transcription coactivator is demonstrated to be a transcription coactivator for ERF, which positively control the expression of ethylene-responsive genes of plants. This transcription coactivator gene can be applied to control ethylene response of plants.
摘要翻译： 本发明的目的是鉴定乙烯应答转录因子（ERFs）中的转录共激活因子（转录辅因子，MBF），以阐明MBF对ERF的作用的机制，该功能正向控制乙烯应答基因的表达 的植物，此外，提供一种控制植物的乙烯应答的方法。 鉴定了乙烯应答转录共激活因子（SEQ ID NO：1和2）。 转录共激活因子被证明是ERF的转录共激活因子，其能正确控制植物乙烯应答基因的表达。 该转录共激活子基因可用于控制植物的乙烯应答。
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公开(公告)号：US4940662A
公开(公告)日：1990-07-10
申请号：US794322
申请日：1985-11-01
申请人： Ken-ichi Yamazaki , Shoji Takao , Koji Yamamoto
发明人： Ken-ichi Yamazaki , Shoji Takao , Koji Yamamoto
摘要： A method of producing a low-molecular weight peptide mixture, comprising the steps of dissolving a first protease in a buffer solution adjusted to an optimum pH for the protease ranging from pH 3 to 10, adding at least one first protein in a buffer solution having a pH of from 3 to 10 and a concentration of 10 to 60% by weight of the protein material and thoroughly mixing the solution, adding a solution of an ester of at least one amino acid pre-formed by esterification of the amino acid with an alcohol, the pH of said buffer solution being optimum for the incorporation of said amino acid in said starting protein in the presence of said first protease in a plastein reaction, reacting said ester of at least one amino acid with said starting protein in a plastein reaction in the mixed solution whereby said at least one amino acid is covalently incorporated into said starting protein to produce a plastein reaction solution containing a modified protein, hydrolyzing said modified protein using at least one second protease having a different specificity from said at least one first protease to produce a low-molecular weight peptide mixture having an amino acid content which has different proportions of amino acids than does said starting protein, and separating from the solution said low-molecular weight peptide mixture which comprises a major proportion of dipeptides and tripeptides and containing not more than 15% by weight of free amino acids and not more than 20% by weight of a high-molecular weight fraction of compounds having a molecular weight of not lower than 700 and removable by gel filtration. The said low-molecular weight peptide mixture is readily absorbable and a useful nutrient.
摘要翻译： 一种生产低分子量肽混合物的方法，包括以下步骤：将第一蛋白酶溶解在缓冲溶液中，调节至最适pH为pH3至10的蛋白酶，加入至少一种第一蛋白质的缓冲溶液中， pH为3至10，浓度为蛋白质材料的10至60重量％，并将溶液充分混合，加入通过氨基酸酯化预先形成的至少一个氨基酸的酯溶液与 醇，所述缓冲溶液的pH最适于在所述起始蛋白质中在所述第一蛋白酶存在下在Plastein反应中引入所述氨基酸，使所述至少一个氨基酸的所述酯与所述起始蛋白质在Plastein反应中反应 在所述混合溶液中，其中所述至少一个氨基酸共价掺入所述起始蛋白质中以产生含有修饰蛋白质的粘多糖反应溶液，水解所述修饰的蛋白质u 与至少一种第一蛋白酶具有不同特异性的至少一种第二蛋白酶，以产生具有与所述起始蛋白质不同比例的氨基酸的氨基酸含量的低分子量肽混合物，并从所述溶液中分离出所述第 低分子量肽混合物，其包含主要比例的二肽和三肽，并且含有不超过15重量％的游离氨基酸和不超过20重量％的高分子量级分的分子量为 不低于700，可通过凝胶过滤去除。 所述低分子量肽混合物易于吸收和有用的营养物质。
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4.发明授权
公开(公告)号：US4452888A
公开(公告)日：1984-06-05
申请号：US279254
申请日：1981-07-01
申请人： Ken-ichi Yamazaki , Shoji Takao , Hiroshi Hara
发明人： Ken-ichi Yamazaki , Shoji Takao , Hiroshi Hara
摘要： A low-molecular weight peptide composition mainly based on dipeptides and tripeptides is produced by dispersing protein raw material from any suitable source in water at a concentration of 5 to 20 w/v %, adjusting the pH of the dispersion to 1 to 4 with an acid, adding at least two acid proteases to the dispersion, and permitting enzymatic proteolysis to take place for a desired period of time at a suitable temperature while supressing the formation of free amino acids. Also provided is a nutrient agent comprising the thus produced low-molecular weight peptide composition mainly based on dipeptides and tripeptides wherein the contents of free amino acids and those peptides having a molecular weight of at least 700 are individually 20% by weight or less. The nutrient agent is useful in medical treatment.
摘要翻译： 主要基于二肽和三肽的低分子量肽组合物通过将来自任何合适的来源的蛋白质原料以5至20w / v％的浓度分散在水中来产生，将分散体的pH调节至1至4， 酸，向分散体中加入至少两种酸性蛋白酶，并允许在合适的温度下进行酶蛋白酶水解所需的时间，同时抑制游离氨基酸的形成。 还提供了一种营养剂，其包含主要基于二肽和三肽的如此制备的低分子量肽组合物，其中游离氨基酸的含量和分子量至少为700的肽分别为20重量％以下。 营养剂可用于医疗。
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