公开(公告)号：US06217540B1

公开(公告)日：2001-04-17

申请号：US09349173

申请日：1999-07-09

申请人： Kenichiro Yazawa ,  Keiichi Ishizaki ,  Takeshi Igarashi ,  Masao Kitajima ,  Shigeru Tezuka ,  Takaki Arai ,  Toshihisa Ito ,  Osamu Seshimoto

发明人： Kenichiro Yazawa ,  Keiichi Ishizaki ,  Takeshi Igarashi ,  Masao Kitajima ,  Shigeru Tezuka ,  Takaki Arai ,  Toshihisa Ito ,  Osamu Seshimoto

IPC分类号： A61M3700

CPC分类号： G01N33/491

摘要： The invention provides a blood filter cartridge which is made so that the filtrate discharged from the filtrate outlet is not drawn back to the inside of the filtering chamber. The blood filter cartridge includes a blood filtering material, a holder containing the blood filtering material and having a blood inlet and a filtrate outlet, and a filtrate receiver which receives the filtrate discharged from the filtrate outlet. The blood filter catridge is provided with a means for preventing the filtrate in the filtrate receiver from returning to inside of the holder upon finishing filtration. Such means include the following: removing opposite faces around the filtrate outlet, making length of lower end of the filtrate outlet 0.8 mm or more, smoothing surface around the filtrate outlet, and forming surface around the filtrate outlet by a material having a small surface energy.

摘要翻译： 本发明提供了一种血液过滤器滤芯，其被制成使得从滤液出口排出的滤液不会被抽回到过滤室的内部。 血液过滤器滤芯包括血液过滤材料，含有血液过滤材料并具有血液入口和滤液出口的保持器和接收从滤​​液出口排出的滤液的滤液接收器。 血液过滤器夹具设置有用于防止滤液接收器中的滤液在完成过滤时返回到保持器内部的装置。 这样的方法包括以下步骤：除去滤液出口周围的相对面，使滤液出口的下端长度为0.8mm以上，使滤液出口周围的表面光滑，并且通过具有小表面能的材料在滤液出口周围形成表面 。

公开(公告)号：US06471868B1

公开(公告)日：2002-10-29

申请号：US09680179

申请日：2000-10-05

申请人： Toshihisa Ito ,  Takaki Arai ,  Kenichiro Yazawa ,  Osamu Seshimoto

发明人： Toshihisa Ito ,  Takaki Arai ,  Kenichiro Yazawa ,  Osamu Seshimoto

IPC分类号： B01D6114

CPC分类号： B01D39/2017 ,  G01N1/4077 ,  Y10T436/25375 ,  Y10T436/255

摘要： A method for preparing a plasma or serum sample from a whole blood sample, by contacting a glass fiber filter which contains calcium, sodium or potassium components with an aqueous solution of an organic acid or a mineral acid to elute the calcium, sodium and potassium components in the glass fiber filter into the aqueous solution, separating the aqueous solution from the filter and washing the glass fiber filter with water to remove any residual aqueous solution adhered thereto and then filtering a whole blood sample through the thus treated fiber filter.

摘要翻译： 通过将含有钙，钠或钾组分的玻璃纤维过滤器与有机酸或无机酸的水溶液接触以洗脱钙，钠和钾组分，从全血样品制备血浆或血清样品的方法 在将玻璃纤维过滤到水溶液中，将水溶液与过滤器分离并用水洗涤玻璃纤维过滤器以除去粘附在其上的残留水溶液，然后通过如此处理的纤维过滤器过滤全血样品。

公开(公告)号：US20060024770A1

公开(公告)日：2006-02-02

申请号：US11230127

申请日：2005-09-19

申请人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

发明人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

IPC分类号： G01N33/537 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/54386 ,  G01N2333/924 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

公开(公告)号：US20050186110A1

公开(公告)日：2005-08-25

申请号：US11060777

申请日：2005-02-18

申请人： Kentaro Nakamura ,  Kazuya Kawasaki ,  Toshihisa Ito ,  Yoshiki Sakaino ,  Osamu Seshimoto

发明人： Kentaro Nakamura ,  Kazuya Kawasaki ,  Toshihisa Ito ,  Yoshiki Sakaino ,  Osamu Seshimoto

IPC分类号： G01N31/22

CPC分类号： G01N31/22

摘要： It is an object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for the analysis of a liquid sample, comprising a water impermeable planar support on one side of which at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in the mentioned order, wherein said spreading layer comprises a non-fibrous porous film such that, when a liquid sample containing a target substance to be measured is caused to become attached to the surface of said liquid sample spreading layer, where said liquid sample is spread and dispersed, 30% or more of said target substance to be measured can be transferred to the at least one functional layer below said liquid sample spreading layer.

摘要翻译： 本发明的目的是提供一种具有很小的批量间和批间差异的多层分析元件，其具有高的测量精度并且尺寸小。 本发明提供了一种用于分析液体样品的多层分析元件，其包括不透水平面支撑体，其一侧上至少一个功能层和至少一个非纤维多孔膜的多孔液体样品铺展层整体层压 其中所述扩散层包括非纤维多孔膜，使得当将含有待测物体的液体样品附着在所述液体样品扩散层的表面上时，其中所述液体样品为 扩散和分散，所述待测量的所述目标物质的30％以上可以转移到所述液体样品扩散层下方的至少一个功能层。

公开(公告)号：US07820402B2

公开(公告)日：2010-10-26

申请号：US11230127

申请日：2005-09-19

申请人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

发明人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

IPC分类号： G01N33/53 ,  G01N33/543

CPC分类号： G01N33/54386 ,  G01N2333/924 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

摘要翻译： 用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。 免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 作为非扩散性底物，使用能够仅与标记酶反应并且不能使片段化酶反应的底物。 当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中 是指具有对内源性葡糖苷酶特异性的反应性的底物。 高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

公开(公告)号：US20080019869A1

公开(公告)日：2008-01-24

申请号：US10589556

申请日：2005-02-18

申请人： Kentaro Nakamura ,  Kazuya Kawasaki ,  Yoshiki Sakaino ,  Toshihisa Ito ,  Osamu Seshimoto

发明人： Kentaro Nakamura ,  Kazuya Kawasaki ,  Yoshiki Sakaino ,  Toshihisa Ito ,  Osamu Seshimoto

IPC分类号： B01L11/00

CPC分类号： G01N33/525

摘要： It is an object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for liquid sample analysis wherein at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in this order on one side of a water-impermeable planar support, and the non-fibrous porous film has a bending rupture strength of 20 gram-weight or more and a tensile percentage of 2% or less when the film is stretched with a tensile force of 50 gram-weight.

摘要翻译： 本发明的目的是提供一种具有很小的批量间和批间差异的多层分析元件，其具有高的测量精度并且尺寸小。 本发明提供了一种用于液体样品分析的多层分析元件，其中至少一个功能层和非纤维多孔膜的至少一个多孔液体样品扩散层在不透水平面支撑体的一侧上依次一体地层叠， 并且当以50克重的拉伸力拉伸膜时，非纤维状多孔膜的弯曲断裂强度为20克重以上，拉伸率为2％以下。

公开(公告)号：US20050186109A1

公开(公告)日：2005-08-25

申请号：US11060775

申请日：2005-02-18

申请人： Kentaro Nakamura ,  Kazuya Kawasaki ,  Yoshiki Sakaino ,  Toshihisa Ito ,  Osamu Seshimoto

发明人： Kentaro Nakamura ,  Kazuya Kawasaki ,  Yoshiki Sakaino ,  Toshihisa Ito ,  Osamu Seshimoto

IPC分类号： G01N31/22

CPC分类号： G01N31/22

摘要： An object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for the analysis of a liquid sample, comprising a water impermeable planar support on one side of which at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in the mentioned order, wherein the arithmetic mean deviation of the profile (Ra) of a contact surface of the at least one functional layer that is in contact with said porous liquid sample spreading layer of said non-fibrous porous film is 1 μm or less and/or the ten-point height of irregularities (Rz) of said contact surface is 8 μm or less.

摘要翻译： 本发明的目的是提供一种具有很高的测量精度并且尺寸小的多批次间差异小的多层分析元件。 本发明提供了一种用于分析液体样品的多层分析元件，其包括不透水平面支撑体，其一侧上至少一个功能层和至少一个非纤维多孔膜的多孔液体样品铺展层整体层压 其中与所述非纤维多孔膜的所述多孔液体样品扩散层接触的所述至少一个功能层的接触表面的轮廓（Ra）的算术平均偏差为1μm以下 和/或所述接触表面的十点高度的不规则（Rz）为8μm或更小。

公开(公告)号：US20050084953A1

公开(公告)日：2005-04-21

申请号：US10961459

申请日：2004-10-07

申请人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

发明人： Yoshiki Sakaino ,  Hitomi Ito ,  Toshihiro Mori ,  Osamu Seshimoto ,  Toshihisa Ito ,  Yoshikazu Amano

IPC分类号： G01N33/543 ,  G01N33/53

CPC分类号： G01N33/54386 ,  G01N2333/924 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

摘要翻译： 用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。 免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 作为非扩散性底物，可以使用能够仅与前体酶反应并且不能使片段化酶反应的底物。 当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中 是指具有对内源性葡糖苷酶特异性的反应性的底物。 高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

公开(公告)号：US06616822B2

公开(公告)日：2003-09-09

申请号：US10095238

申请日：2002-03-11

申请人： Masaaki Terashima ,  Toshihisa Ito ,  Osamu Seshimoto

发明人： Masaaki Terashima ,  Toshihisa Ito ,  Osamu Seshimoto

IPC分类号： G01N2730

CPC分类号： G01N27/301

摘要： A silver/silver halide electrode composed of a non-electroconductive support sheet, a silver metal layer and a silver halide layer in order, is improved in its sensitivity by making the silver halide layer almost free from heavy metal element, for instance, by oxidizing the silver metal layer on its surface side with an oxidizing compound containing no heavy metal element or a chelated ferric oxidizing compound in the presence of a halide containing no heavy metal element.

摘要翻译： 由非导电性支持片，银金属层和卤化银层构成的银/卤化银电极的顺序通过使卤化银层几乎不含重金属元素而提高其灵敏度，例如通过氧化 在不含重金属元素的卤化物的存在下，其表面侧的银金属层与不含重金属元素的氧化化合物或螯合的铁氧化化合物反应。

公开(公告)号：US20080279725A1

公开(公告)日：2008-11-13

申请号：US11664284

申请日：2005-09-29

申请人： Mitsuharu Nirasawa ,  Shigeki Kageyama ,  Toshihisa Ito

发明人： Mitsuharu Nirasawa ,  Shigeki Kageyama ,  Toshihisa Ito

IPC分类号： G01N33/00 ,  B32B37/12

CPC分类号： G01N33/525 ,  B01L3/5023 ,  G01N31/22

摘要： The object of the invention is to provide a multilayer analytical element having a non-fibrous porous film with high film strength, which does not produce uneven coloration in the form of a white-out and which has high accuracy of analysis. The present invention provides a dry multilayer analytical element for the analysis of a liquid sample which comprises a water-non-transmitting planar support on one side of which at least one adhesion layer and a porous liquid-sample-developing layer are integrally layered in the mentioned order, wherein the porous liquid-sample-developing layer comprises a non-fibrous porous film whose bending fracture strength is 20 g weight or more and whose elongation ratio when pulled with a 50-g weight is 2% or less, and wherein a adhesion layer polymer binder is caused to become seeped up in the non-fibrous porous film to 2 to 50 μm.

摘要翻译： 本发明的目的是提供一种具有高膜强度的非纤维多孔膜的多层分析元件，其不会产生白色形式的不均匀着色，并且分析精度高。 本发明提供了一种用于分析液体样品的干燥多层分析元件，该液体样品在其一侧包括水不透过平面支撑体，其中至少一个粘附层和多孔液体样品显影层一体地层叠在 其中多孔液体样品显影层包括弯曲断裂强度为20g或更大的非纤维多孔膜，其拉伸50g时的伸长率为2％以下，其中， 使粘合层聚合物粘合剂在非纤维多孔膜中渗透至2〜50μm。