摘要:
It is an object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for liquid sample analysis wherein at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in this order on one side of a water-impermeable planar support, and the non-fibrous porous film has a bending rupture strength of 20 gram-weight or more and a tensile percentage of 2% or less when the film is stretched with a tensile force of 50 gram-weight.
摘要:
It is an object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for the analysis of a liquid sample, comprising a water impermeable planar support on one side of which at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in the mentioned order, wherein said spreading layer comprises a non-fibrous porous film such that, when a liquid sample containing a target substance to be measured is caused to become attached to the surface of said liquid sample spreading layer, where said liquid sample is spread and dispersed, 30% or more of said target substance to be measured can be transferred to the at least one functional layer below said liquid sample spreading layer.
摘要:
An object of the invention to provide a multilayer analysis element with small intra-lot and lot-to-lot differences that has high measurement accuracy and which is small in size. The present invention provides a multilayer analysis element for the analysis of a liquid sample, comprising a water impermeable planar support on one side of which at least one functional layer and at least one porous liquid sample spreading layer of non-fibrous porous film are integrally laminated in the mentioned order, wherein the arithmetic mean deviation of the profile (Ra) of a contact surface of the at least one functional layer that is in contact with said porous liquid sample spreading layer of said non-fibrous porous film is 1 μm or less and/or the ten-point height of irregularities (Rz) of said contact surface is 8 μm or less.
摘要:
The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
摘要:
The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
摘要:
The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
摘要:
A glass fiber filter for blood filtration, in which a glass fiber is cleaned with an organic acid and then the surface of a glass fiber is coated with a biocompatible polymer such as poly (alkoxy acrylate), a blood filtration device and a dry-type blood analysis element in which the glass fiber filter for blood filtration is used.
摘要:
This invention relates to a dry analytical element using a water-soluble indicator which improves measuring accuracy, which comprises a water-impermeable support, a water-permeable layer and a spreading layer having a function to spread a liquid uniformly, wherein a water-soluble indicator for colorimetry is incorporated into the s preading layer.
摘要:
An analysis element for use in a blood test method enabling easy and simple operations that are performed quickly up to measurement, an analysis element for use in a blood test method that operations up to the measurement for many components can be quickly performed, and that is safe and has a sufficient measurement accuracy thereof, and an analysis element for use in a test method using body fluids and urines of humans and animals, plain water, seawater, soil extract, agricultural products, marine products, processed-food extracts, and liquid for use in scientific research as specimens, are provided, the present invention relates to a multi-component measurement dry analysis element for use in a method for testing a specimen, the method using an area sensor as a detector to obtain a result of measurement according to information represented by 1000 pixels or more per one component, and to perform simultaneous measurements of plural components.
摘要:
A microdevice for performing a method for separating and purifying a nucleic acid, the microdevice comprising: at least one opening; and at least one channel for passing a sample solution, wherein the method comprises: (A) a step of bringing a nucleic acid-containing sample solution into contact with a nucleic acid-adsorbing support having a function of adsorbing a nucleic acid; (B) a step of washing the nucleic acid-adsorbing support with a washing solution in a state of a nucleic acid being adsorbed to the support; and (C) a step of desorbing the nucleic acid from the nucleic acid-adsorbing support by a recovering solution, thereby purifying the nucleic acid; an apparatus for utilizing the microdevice; and a reagent kit for use in the microdevice.