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48 条记录 (耗时 0.038 秒)

    Solid-phase clonal amplification and related methods
    7.
    发明授权
    Solid-phase clonal amplification and related methods 有权
    固相克隆扩增及相关方法

    公开(公告)号：US08951940B2

    公开(公告)日：2015-02-10

    申请号：US13637770

    申请日：2011-03-16

    申请人： Neil A. Straus ,  Shengrong Lin ,  Helmy A. Eltoukhy ,  Kevin Gunderson

    发明人： Neil A. Straus ,  Shengrong Lin ,  Helmy A. Eltoukhy ,  Kevin Gunderson

    IPC分类号： C40B50/06 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2531/125 ,  C12Q2563/149

    摘要： The present invention provides methods and compositions for analyzing nucleic acid sequences. In some aspects, the methods utilize clonal objects, such as DNA balls, that have been captured on beads. Using the methods described here, compositions are fabricated wherein a bead and one clonal object are affinity bound or hybridized to each other through an affinity binding patch or hybridization patch on the surface of the bead. The invention also provides a population of beads having affinity bound or hybridized clonal objects at a ratio of 1:1. The invention additionally provides methods for amplifying a target nucleic acid molecule utilizing the compositions described herein.

    摘要翻译： 本发明提供了用于分析核酸序列的方法和组合物。 在某些方面，该方法利用已被捕获在珠上的克隆物体，例如DNA球。 使用本文描述的方法，制备组合物，其中珠粒和一个克隆物体通过珠粒表面上的亲和结合片段或杂交片段彼此亲和地结合或杂交。 本发明还提供了以1:1的比例具有亲和结合或杂交的克隆物体的珠群。 本发明另外提供了利用本文所述组合物扩增靶核酸分子的方法。

    Nucleic acid sequencing system and method
    8.
    发明授权
    Nucleic acid sequencing system and method 有权
    核酸测序系统及方法

    公开(公告)号：US08914241B2

    公开(公告)日：2014-12-16

    申请号：US12878687

    申请日：2010-09-09

    申请人： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

    发明人： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

    IPC分类号： G06F19/20 ,  C12Q1/68 ,  C12M1/34 ,  C12M3/00 ,  G06F19/22

    CPC分类号： C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/501 ,  C12Q2537/143

    摘要： A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

    摘要翻译： 公开了以自动或半自动方式测序核酸的技术。 多个核酸位点的样品阵列在多个周期内进行处理以向要测序的材料添加核苷酸，检测添加到位点的核苷酸，以及去除阻断剂的添加核苷酸和用于鉴定最后添加的标签 核苷酸。 对系统的多个参数进行监控，以便在样品排序期间发生问题的诊断和纠正。 质量控制程序在排序过程中运行，以确定样品的质量和收集的数据的质量。

    MULTI-SAMPLE INDEXING FOR MULTIPLEX GENOTYPING
    9.
    发明申请
    MULTI-SAMPLE INDEXING FOR MULTIPLEX GENOTYPING 审中-公开
    多重基因多样性指数

    公开(公告)号：US20120202704A1

    公开(公告)日：2012-08-09

    申请号：US13501502

    申请日：2010-12-07

    申请人： Jian-Bing Fan ,  Kevin Gunderson

    发明人： Jian-Bing Fan ,  Kevin Gunderson

    IPC分类号： C40B30/04

    CPC分类号： C12N15/1065 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2533/101 ,  C12Q2533/107 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2565/102 ,  C12Q2525/186

    摘要： A method for determining the presence of multiple nucleotide sequences of interest in multiple samples while preserving the identity of each sample, by contacting the samples with a plurality of probe sets. The probes are designed to indicate the presence of the sequences of interest and the identity of the sample containing the sequence of interest in complex mixtures. Applications of the method include genotyping, expression analysis, and identification of individual species in complex samples. Kits of probe sets for use in the methods are also provided.

    摘要翻译： 通过使样本与多个探针组接触，确定多个样品中多个核苷酸序列的存在同时保持每个样品的身份的方法。 探针被设计为指示感兴趣的序列的存在和在复杂混合物中含有感兴趣的序列的样品的身份。 该方法的应用包括基因分型，表达分析和复杂样本中个体物种的鉴定。 还提供了用于方法的探针组的套件。